FastQCFastQC Report
Thu 26 May 2016
SRR1547088_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547088_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1479820
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78180.5283074968577259No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGA700.045.00000414
CGCGAGT207.0339453E-445.04
GCGTAAG1150.043.0434761
CGTTTTT50700.042.4704171
CGCATCG750.039.021
GACCGAT7900.038.4493689
CGAGGGT6050.038.3057864
GCGATAC1250.037.8000039
ACGGGTA4900.037.653065
CACGACG1500.037.526
TTACGGG4200.037.53
TATAGCG1200.037.4999961
CGAGGGA6850.037.4452554
TAGGGTG17550.037.3076935
TACGGGT4650.037.2580644
GGGCGAT32850.037.191787
CGATACG850.037.05882310
TCGATAG1050.036.428571
CTAGGGT8900.036.404494
TAAGGGA12350.036.2550624
Produced by FastQC (version 0.11.5)