##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547088_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1479820
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.414926139665635	31.0	31.0	33.0	30.0	34.0
2	31.799141111756835	31.0	31.0	34.0	30.0	34.0
3	31.93117473746807	33.0	31.0	34.0	30.0	34.0
4	35.68930140152181	37.0	35.0	37.0	33.0	37.0
5	35.62491789541971	37.0	35.0	37.0	33.0	37.0
6	35.68218702274601	37.0	35.0	37.0	33.0	37.0
7	36.03691327323593	37.0	35.0	37.0	35.0	37.0
8	34.73556716357395	37.0	35.0	37.0	33.0	37.0
9	37.04058872024976	39.0	37.0	39.0	34.0	39.0
10	36.71098917435904	39.0	37.0	39.0	32.0	39.0
11	36.83330067170331	39.0	37.0	39.0	32.0	39.0
12	36.697904474868565	39.0	35.0	39.0	33.0	39.0
13	36.663924666513495	39.0	35.0	39.0	33.0	39.0
14	37.791705072238514	40.0	36.0	41.0	33.0	41.0
15	37.92329269776054	40.0	36.0	41.0	33.0	41.0
16	37.93894054682326	40.0	36.0	41.0	33.0	41.0
17	37.84827005987215	40.0	36.0	41.0	33.0	41.0
18	37.79985268478599	39.0	36.0	41.0	33.0	41.0
19	37.76377870281521	40.0	36.0	41.0	33.0	41.0
20	37.64342149720912	40.0	35.0	41.0	33.0	41.0
21	37.57125731507886	39.0	35.0	41.0	33.0	41.0
22	37.478227081672095	39.0	35.0	41.0	32.0	41.0
23	37.39787609303834	39.0	35.0	41.0	32.0	41.0
24	37.28114094957495	39.0	35.0	41.0	32.0	41.0
25	37.18096863132003	39.0	35.0	41.0	32.0	41.0
26	37.09595626495114	39.0	35.0	41.0	32.0	41.0
27	37.01175953832223	39.0	35.0	41.0	31.0	41.0
28	37.04271803327432	39.0	35.0	41.0	31.0	41.0
29	37.003727480369236	39.0	35.0	41.0	31.0	41.0
30	36.96966252652349	39.0	35.0	41.0	31.0	41.0
31	36.90164141584788	39.0	35.0	41.0	31.0	41.0
32	36.842989012177156	39.0	35.0	41.0	31.0	41.0
33	36.72476990444784	39.0	35.0	41.0	31.0	41.0
34	36.57907583354732	39.0	35.0	41.0	30.0	41.0
35	36.46658715249152	39.0	35.0	41.0	30.0	41.0
36	36.35593315403225	39.0	35.0	41.0	30.0	41.0
37	36.32142422727088	39.0	35.0	41.0	30.0	41.0
38	36.210338419537514	39.0	35.0	41.0	30.0	41.0
39	36.140271789812275	39.0	35.0	41.0	30.0	41.0
40	36.005473638685785	39.0	35.0	41.0	29.0	41.0
41	35.92149450608858	39.0	35.0	41.0	29.0	41.0
42	35.853175386195616	38.0	35.0	41.0	29.0	41.0
43	35.78085240096769	38.0	35.0	41.0	29.0	41.0
44	35.675415928964334	38.0	35.0	40.0	28.0	41.0
45	35.55092511251368	38.0	35.0	40.0	27.0	41.0
46	35.48779986755146	38.0	35.0	40.0	27.0	41.0
47	35.41892054439053	38.0	34.0	40.0	27.0	41.0
48	35.33982781689665	38.0	34.0	40.0	27.0	41.0
49	35.27792974821262	38.0	34.0	40.0	27.0	41.0
50	35.1942351096755	38.0	34.0	40.0	27.0	41.0
51	34.839101377194524	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	1.0
12	1.0
13	9.0
14	23.0
15	24.0
16	90.0
17	193.0
18	473.0
19	935.0
20	1617.0
21	2592.0
22	4072.0
23	6012.0
24	9039.0
25	13242.0
26	17680.0
27	21499.0
28	23576.0
29	26588.0
30	31322.0
31	37963.0
32	47121.0
33	60861.0
34	94214.0
35	142339.0
36	115403.0
37	153933.0
38	234980.0
39	433733.0
40	282.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.12296090065008	25.194753415955994	28.533267559568053	14.14901812382587
2	31.11182441107702	25.96248192347718	29.21253936289549	13.71315430255031
3	29.11293265397143	26.329959049073537	31.135273208903786	13.421835088051248
4	25.05183062804936	29.036031409225448	30.750631833601382	15.161506129123811
5	22.038558743630983	32.66336446324553	30.223608276682302	15.07446851644119
6	19.76889081104459	41.88597261829141	27.66329688745929	10.681839683204714
7	89.13259720776851	3.2498547120595753	6.171628981903204	1.4459190982687082
8	86.98517387249801	5.3232149856063575	5.572029030557771	2.1195821113378654
9	84.01893473530565	3.8668216404697873	9.18773904934384	2.926504574880729
10	34.03927504696517	22.644443243097136	27.888324255652712	15.427957454284979
11	31.935640821180954	25.422483815599193	24.075090213674635	18.566785149545215
12	28.291886851103516	24.56319011771702	27.03626116689868	20.10866186428079
13	23.882972253382167	28.36203051722507	28.754240380586825	19.000756848805935
14	21.016610128258844	30.656025732859405	29.277682420834967	19.04968171804679
15	20.593112675865985	25.075549729021095	35.39585895581895	18.93547863929397
16	22.061939965671503	22.757700260842533	35.00148666729737	20.17887310618859
17	21.239812950223676	23.10456677163439	30.091767917719725	25.563852360422214
18	23.068278574421214	24.254233623008204	31.481328810260706	21.196158992309876
19	26.178454136313878	25.873552188779712	27.31609249773621	20.6319011771702
20	27.201889418983388	25.608925409847146	26.660607371166762	20.528577800002704
21	24.963171196496873	26.973956291981455	28.58901758321958	19.47385492830209
22	23.949872281763998	23.46738116797989	28.856617696746902	23.72612885350921
23	21.773999540484652	27.628022327039776	28.07632009298428	22.521658039491292
24	21.52072549364112	26.579854306604854	32.13296211701423	19.766458082739792
25	22.388939195307536	26.77237772161479	29.953913313781406	20.884769769296266
26	20.459853225392276	29.231798461975107	27.837912719114488	22.470435593518133
27	20.923625846386724	28.017056128448054	29.03400413563812	22.025313889527105
28	19.97378059493722	27.816153315943833	32.07727966914895	20.132786419969996
29	19.985876660674947	26.614250381803195	31.22089173007528	22.17898122744658
30	21.60870916733116	27.81743725588247	30.088253976835023	20.485599599951346
31	24.800651430579396	27.112419077995973	27.09829573867092	20.988633752753714
32	24.56933951426525	27.69884175102377	26.947669311132433	20.784149423578544
33	23.753294319579407	26.706694057385356	26.98571447878796	22.554297144247272
34	20.541282047816626	28.762281899149894	28.516576340365717	22.179859712667756
35	20.914503115243747	26.907394142530848	28.72356097363193	23.45454176859348
36	23.993458663891552	28.043072806152097	26.03850468300199	21.92496384695436
37	21.542417321025532	28.618615777594574	27.691340838750662	22.14762606262924
38	22.053628143963454	29.630765903961294	26.16068170453163	22.15492424754362
39	21.8082604641105	27.90961062831966	26.369963914530146	23.912164993039696
40	22.727156005460124	26.382803313916558	27.97617277777027	22.91386790285305
41	21.130340176507953	25.141368544823017	28.568609695773812	25.159681582895217
42	22.311632495844087	27.902920625481475	26.507413063750995	23.27803381492344
43	21.706829208957846	26.79555621629658	27.89217607546864	23.60543849927694
44	22.013623278506845	27.341568569150304	28.487248449135706	22.15755970320715
45	20.75374031976862	28.66950034463651	27.634779905664203	22.94197942993067
46	22.429822545985324	27.65876930978092	27.976713384060226	21.934694760173535
47	21.660607371166762	27.24175913286751	28.62111608168561	22.476517414280114
48	21.725007095457556	27.379411009447097	29.013393520833613	21.882188374261734
49	22.117825140895516	26.095741373950887	29.081239610222866	22.705193874930735
50	20.96444162127826	27.575313213769242	28.86351042694382	22.596734738008678
51	20.56250084469733	28.526915435661092	27.976578232487736	22.934005487153843
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	862.0
1	1266.5
2	1671.0
3	7776.0
4	13881.0
5	9808.5
6	5736.0
7	5824.5
8	5913.0
9	6255.5
10	6598.0
11	6745.0
12	6892.0
13	6776.0
14	6660.0
15	6578.5
16	6497.0
17	6416.0
18	6335.0
19	6266.0
20	6197.0
21	6294.5
22	6392.0
23	6819.0
24	7246.0
25	8679.5
26	12406.5
27	14700.0
28	16137.0
29	17574.0
30	21213.0
31	24852.0
32	29081.5
33	33311.0
34	37202.5
35	41094.0
36	44475.0
37	47856.0
38	54820.5
39	61785.0
40	71859.5
41	81934.0
42	91421.0
43	100908.0
44	106504.5
45	112101.0
46	117543.0
47	122985.0
48	126379.0
49	129773.0
50	124519.0
51	119265.0
52	110938.5
53	102612.0
54	94190.5
55	85769.0
56	77970.5
57	70172.0
58	64081.5
59	57991.0
60	52322.0
61	46653.0
62	41784.5
63	36916.0
64	31349.0
65	25782.0
66	21636.5
67	17491.0
68	14542.0
69	11593.0
70	10429.5
71	9266.0
72	8178.0
73	7090.0
74	5800.5
75	3476.5
76	2442.0
77	1790.5
78	1139.0
79	923.5
80	708.0
81	513.5
82	319.0
83	224.0
84	129.0
85	93.0
86	57.0
87	43.5
88	30.0
89	17.0
90	4.0
91	4.0
92	4.0
93	2.5
94	1.0
95	5.5
96	10.0
97	5.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1479820.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.367532769866457
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.15901807748688	20.08125404878987
2	6.312069716631107	3.328674101979048
3	2.5933849955032025	2.05143491561432
4	1.6713182913342677	1.7627415926253407
5	1.2159602177786704	1.6030935444566523
6	0.9295851936708115	1.4706520833898669
7	0.8418046424155236	1.5537418046301923
8	0.7259210267434187	1.531259716879376
9	0.654798083028345	1.5538868920706062
>10	7.971052238106827	47.246899728396755
>50	0.8744589599142231	14.78032341087226
>100	0.04799165338943107	1.991717798849841
>500	0.001845832798105911	0.33533669129517063
>1k	5.273807994588317E-4	0.16540866443939684
>5k	2.6369039972941587E-4	0.5435750057113091
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7818	0.5283074968577259	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0272735873281885E-4	0.0	0.0	0.047911232447189527	0.0
2	2.0272735873281885E-4	0.0	0.0	0.14481490992147694	0.0
3	2.0272735873281885E-4	0.0	0.0	0.23192009839034478	0.0
4	2.0272735873281885E-4	0.0	0.0	0.39835925990998905	0.0
5	2.0272735873281885E-4	0.0	0.0	0.6412266356719061	0.0
6	2.0272735873281885E-4	0.0	0.0	1.2179183954805315	0.0
7	2.0272735873281885E-4	0.0	0.0	1.4607857712424483	0.0
8	2.0272735873281885E-4	0.0	0.0	2.1157978673081863	0.0
9	2.0272735873281885E-4	0.0	0.0	2.4334716384425135	0.0
10	2.0272735873281885E-4	0.0	0.0	2.9583327702017814	0.0
11	2.0272735873281885E-4	0.0	0.0	3.367774459055831	0.0
12	2.0272735873281885E-4	0.0	0.0	3.7078158154370127	0.0
13	2.0272735873281885E-4	0.0	0.0	3.8818234650160153	0.0
14	2.0272735873281885E-4	0.0	0.0	3.960751983349326	0.0
15	2.0272735873281885E-4	0.0	0.0	4.049884445405523	0.0
16	2.0272735873281885E-4	0.0	0.0	4.226257247503074	0.0
17	2.0272735873281885E-4	0.0	0.0	4.414523387979619	0.0
18	2.0272735873281885E-4	0.0	0.0	4.66583773702207	0.0
19	2.0272735873281885E-4	0.0	0.0	4.7841629387357925	0.0
20	6.081820761984566E-4	0.0	0.0	4.9124893568136665	0.0
21	6.081820761984566E-4	0.0	0.0	5.080212458271952	0.0
22	6.081820761984566E-4	0.0	0.0	5.258680109743077	0.0
23	6.081820761984566E-4	0.0	0.0	5.4563392845075755	0.0
24	6.081820761984566E-4	0.0	0.0	5.624805719614548	0.0
25	6.757578624427296E-4	0.0	0.0	5.7711072968333985	0.0
26	6.757578624427296E-4	0.0	0.0	5.906123717749456	0.0
27	6.757578624427296E-4	0.0	0.0	6.048438323579894	0.0
28	6.757578624427296E-4	0.0	0.0	6.194469597653769	0.0
29	6.757578624427296E-4	0.0	0.0	6.355570272060115	0.0
30	6.757578624427296E-4	0.0	0.0	6.568231271370842	0.0
31	6.757578624427296E-4	0.0	0.0	6.732778310875647	0.0
32	6.757578624427296E-4	0.0	0.0	6.9005014123339325	0.0
33	6.757578624427296E-4	0.0	0.0	7.064102390831318	0.0
34	6.757578624427296E-4	0.0	0.0	7.234393372166886	0.0
35	6.757578624427296E-4	0.0	0.0	7.429011636550391	0.0
36	6.757578624427296E-4	0.0	0.0	7.598762011596005	0.0
37	6.757578624427296E-4	0.0	0.0	7.783446635401603	0.0
38	6.757578624427296E-4	0.0	0.0	7.978605506075064	0.0
39	6.757578624427296E-4	0.0	0.0	8.212890756983958	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTCGA	70	0.0	45.000004	14
CGCGAGT	20	7.0339453E-4	45.0	4
GCGTAAG	115	0.0	43.043476	1
CGTTTTT	5070	0.0	42.470417	1
CGCATCG	75	0.0	39.0	21
GACCGAT	790	0.0	38.449368	9
CGAGGGT	605	0.0	38.305786	4
GCGATAC	125	0.0	37.800003	9
ACGGGTA	490	0.0	37.65306	5
CACGACG	150	0.0	37.5	26
TTACGGG	420	0.0	37.5	3
TATAGCG	120	0.0	37.499996	1
CGAGGGA	685	0.0	37.445255	4
TAGGGTG	1755	0.0	37.307693	5
TACGGGT	465	0.0	37.258064	4
GGGCGAT	3285	0.0	37.19178	7
CGATACG	85	0.0	37.058823	10
TCGATAG	105	0.0	36.42857	1
CTAGGGT	890	0.0	36.40449	4
TAAGGGA	1235	0.0	36.255062	4
>>END_MODULE