FastQCFastQC Report
Thu 26 May 2016
SRR1547087_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547087_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences607676
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35510.5843574536430598No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC12030.19796733785767415No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTAGG351.2106648E-745.0000042
GCTACGA351.2106648E-745.00000410
CGGGTCA253.8886283E-545.06
AGATCTA207.0306583E-445.013
CGCATCG207.0306583E-445.021
GTAGGAC207.0306583E-445.014
TAACGCA207.0306583E-445.013
GACTTAG207.0306583E-445.01
CGTAGTG207.0306583E-445.033
TATAGCG207.0306583E-445.01
TAATGCG502.1827873E-1145.01
ATACCGG253.8886283E-545.02
CGGACCG207.0306583E-445.030
ATTCGCA302.1638061E-644.99999614
CGAATAT302.1638061E-644.99999614
TAGTGCG302.1638061E-644.9999961
ATAACGG750.042.02
CGTATGG650.041.538462
TAGGGCG1800.041.255
AATGCGG1200.041.2499962
Produced by FastQC (version 0.11.5)