##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547087_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	607676
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.45420750531533	31.0	31.0	33.0	30.0	34.0
2	31.80845713834346	31.0	31.0	34.0	30.0	34.0
3	31.919383026481217	33.0	31.0	34.0	30.0	34.0
4	35.65437667441202	37.0	35.0	37.0	33.0	37.0
5	35.579851763110604	37.0	35.0	37.0	33.0	37.0
6	35.62576109637372	37.0	35.0	37.0	33.0	37.0
7	36.007082721713545	37.0	35.0	37.0	35.0	37.0
8	34.68216944555981	37.0	35.0	37.0	33.0	37.0
9	37.005851802605335	39.0	37.0	39.0	33.0	39.0
10	36.91208637497614	39.0	37.0	39.0	32.0	39.0
11	36.78128147236356	39.0	35.0	39.0	32.0	39.0
12	36.0820552399634	37.0	35.0	39.0	32.0	39.0
13	35.748839842284376	38.0	35.0	39.0	31.0	39.0
14	36.768065548088124	39.0	35.0	41.0	31.0	41.0
15	37.089465438819374	39.0	35.0	41.0	32.0	41.0
16	37.24132926098776	39.0	35.0	41.0	33.0	41.0
17	37.16056418222869	38.0	35.0	41.0	32.0	41.0
18	37.12309026520712	38.0	35.0	41.0	32.0	41.0
19	37.04677163488438	38.0	35.0	41.0	32.0	41.0
20	36.88701874024974	38.0	35.0	41.0	32.0	41.0
21	36.75564610088271	38.0	35.0	41.0	32.0	41.0
22	36.66059051204918	38.0	35.0	41.0	32.0	41.0
23	36.62023347968325	38.0	35.0	41.0	32.0	41.0
24	36.48656191786412	38.0	35.0	40.0	31.0	41.0
25	36.39184367985571	38.0	35.0	40.0	31.0	41.0
26	36.25386390115786	37.0	35.0	40.0	31.0	41.0
27	36.154488576149134	37.0	35.0	40.0	31.0	41.0
28	36.21138073578683	37.0	35.0	40.0	31.0	41.0
29	36.23441932872122	37.0	35.0	40.0	31.0	41.0
30	36.21634884379176	37.0	35.0	40.0	31.0	41.0
31	36.10273073150824	37.0	35.0	40.0	31.0	41.0
32	35.94310125790717	37.0	35.0	40.0	30.0	41.0
33	35.81976085940534	37.0	35.0	40.0	30.0	41.0
34	35.72967173296296	37.0	35.0	40.0	30.0	41.0
35	35.62749063645759	37.0	35.0	40.0	30.0	41.0
36	35.470838078186404	37.0	35.0	40.0	29.0	41.0
37	35.3812887130642	37.0	34.0	40.0	29.0	41.0
38	35.312421421941956	37.0	34.0	40.0	28.0	41.0
39	35.276061585450144	37.0	34.0	40.0	28.0	41.0
40	35.113280761458405	37.0	34.0	40.0	27.0	41.0
41	35.0670143300048	37.0	34.0	40.0	27.0	41.0
42	35.01052699135724	36.0	34.0	40.0	27.0	41.0
43	34.90334158334375	36.0	34.0	40.0	27.0	41.0
44	34.78223099151521	36.0	34.0	40.0	26.0	41.0
45	34.67859188119985	36.0	34.0	40.0	26.0	41.0
46	34.63177910597094	36.0	34.0	40.0	26.0	41.0
47	34.56736320012638	36.0	34.0	40.0	26.0	41.0
48	34.53599286461865	35.0	34.0	40.0	26.0	41.0
49	34.50805857068569	36.0	34.0	40.0	26.0	41.0
50	34.38239456552505	35.0	34.0	40.0	25.0	41.0
51	34.03041258828718	35.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	5.0
14	9.0
15	20.0
16	50.0
17	136.0
18	266.0
19	546.0
20	983.0
21	1438.0
22	2291.0
23	3302.0
24	4730.0
25	6499.0
26	8547.0
27	10253.0
28	11280.0
29	12764.0
30	14747.0
31	18043.0
32	22573.0
33	30016.0
34	53257.0
35	93977.0
36	41802.0
37	51505.0
38	76388.0
39	142114.0
40	133.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.7215489833398	24.274778006700938	29.166364970806814	13.837308039152443
2	29.821154694277872	26.961077942851126	29.885827315872277	13.33194004699873
3	28.41876263008577	25.941126521369938	32.19857292372909	13.441537924815197
4	24.99358210625399	29.227088119326744	30.666012809457673	15.11331696496159
5	22.699761056878994	32.37926131688597	30.766230688722278	14.154746937512751
6	21.059577801328338	41.49793640031859	27.004357585292162	10.438128213060907
7	87.24797424943556	4.274481796220354	6.938401384948559	1.5391425693955332
8	85.009281261725	6.218774478504993	5.916310665552038	2.8556335942179714
9	83.05478577399799	5.514287218846885	8.514570264417221	2.9163567427379062
10	48.92475595547627	26.956634785642347	13.275166371553263	10.843442887328116
11	43.41738031450971	20.74510100777388	22.244748846424738	13.592769831291676
12	38.81821891929252	21.44037283025823	24.331551682146408	15.409856568302846
13	21.303786886432903	38.54455334750755	25.73460199185092	14.417057774208624
14	16.160256452451634	40.55483514241141	27.824037809622233	15.460870595514715
15	14.704217378998019	24.243511344861407	45.69918838328319	15.353082892857378
16	16.14824347185013	19.368874202700123	45.865230813788926	18.617651511660817
17	16.958379136250237	20.64603505815599	28.72896082780956	33.66662497778422
18	20.388167378668896	24.24433415175192	34.169524549266384	21.197973920312798
19	27.83736728124856	25.74464023591519	25.92302476977863	20.494967713057616
20	29.45632211902395	23.99782120735392	25.638004462904572	20.907852210717554
21	21.84404189074441	29.478044220933526	28.266543355340673	20.41137053298139
22	22.70354596857536	24.705106010439774	26.02044510561549	26.570902915369377
23	19.617197322257255	30.674734562497118	25.689018490116446	24.01904962512918
24	20.45004245683555	24.16912960195894	37.509462279240914	17.8713656619646
25	18.13499298968529	25.741349008353136	33.93979686543487	22.1838611365267
26	17.2613366333375	33.833490215180454	27.719376773148852	21.18579637833319
27	18.27964244103766	32.934491406604835	28.367584041495796	20.41828211086171
28	15.647647759661398	29.756975756817777	36.20662984880101	18.38874663471982
29	16.318729059564635	25.24470276923887	35.98496567249653	22.451602498699966
30	18.86943042015811	29.656264193418863	30.85427102600728	20.620034360415747
31	27.12860142575978	26.819719719060814	25.90475845680922	20.146920398370185
32	27.966218840303057	27.470395408079305	26.320275936518804	18.243109815098833
33	25.700208663827436	27.53589083656422	26.448469250060885	20.315431249547455
34	19.441116647687252	28.00768830758496	28.7078969714124	23.843298073315385
35	19.481434185322442	25.680132175698894	31.344663932753637	23.493769706225027
36	28.338292116193497	25.353806962921034	26.41506329030602	19.892837630579454
37	20.36677439951553	30.6205938691013	29.035538675215083	19.97709305616809
38	20.749544164982655	31.39600708272171	25.85999776196526	21.994450990330375
39	20.059373745219492	30.833207169610123	26.928494789986768	22.178924295183617
40	23.88229911992575	25.427201337554884	26.969799695890572	23.720699846628797
41	17.478063968298898	24.143128904218695	28.315582646015308	30.0632244814671
42	22.37557514201647	26.545066779007232	25.03735543282934	26.042002646146962
43	22.623240016061192	26.504913802750153	26.869746378004066	24.002099803184592
44	20.557830159492887	29.6352003370217	28.470764025566254	21.336205477919155
45	18.17596877283289	33.9885070333533	24.451023242649043	23.384500951164767
46	22.563504235809873	30.525641953935978	26.36240364931312	20.54845016094103
47	22.520882838881246	26.174145432763513	27.61833608699373	23.686635641361516
48	22.93952698477478	23.31308131306815	30.946425397744854	22.80096630441222
49	20.928422382980404	23.9030338535667	31.915362792014168	23.253180971438727
50	18.914191115002073	30.625201587688178	27.997485502142588	22.46312179516716
51	18.461811886597463	31.670824584153394	25.792363035565003	24.075000493684133
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	724.0
1	1008.0
2	1292.0
3	3589.5
4	5887.0
5	4187.5
6	2488.0
7	2579.5
8	2671.0
9	2875.0
10	3079.0
11	3223.5
12	3368.0
13	3306.5
14	3245.0
15	3176.5
16	3108.0
17	2992.5
18	2877.0
19	2688.5
20	2500.0
21	2624.0
22	2748.0
23	3096.5
24	3445.0
25	3483.0
26	4378.5
27	5236.0
28	6120.5
29	7005.0
30	7442.0
31	7879.0
32	9777.0
33	11675.0
34	13342.0
35	15009.0
36	15912.5
37	16816.0
38	19520.0
39	22224.0
40	30918.0
41	39612.0
42	47677.5
43	55743.0
44	57161.0
45	58579.0
46	59321.5
47	60064.0
48	57877.5
49	55691.0
50	51946.0
51	48201.0
52	42082.0
53	35963.0
54	31203.5
55	26444.0
56	24889.0
57	23334.0
58	21389.5
59	19445.0
60	18265.5
61	17086.0
62	14825.5
63	12565.0
64	10967.5
65	9370.0
66	7866.0
67	6362.0
68	5242.0
69	4122.0
70	3519.0
71	2916.0
72	2673.0
73	2430.0
74	1846.5
75	969.5
76	676.0
77	572.0
78	468.0
79	393.0
80	318.0
81	237.0
82	156.0
83	104.5
84	53.0
85	30.5
86	8.0
87	6.5
88	5.0
89	3.5
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	607676.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.29454843671216
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.0094812154939	21.565281119957977
2	6.419762546472753	3.504490395537823
3	2.3066444817033824	1.8887645859638333
4	1.2657341228957908	1.3819056530151415
5	0.8285311590434373	1.1307191925918183
6	0.630379566872601	1.0323555372910704
7	0.527766739367898	1.0083608381673912
8	0.4515205065722746	0.9859238669444609
9	0.3841727502959516	0.9437239566915998
>10	7.316086193495288	49.89162123660424
>50	0.8091211308997219	13.554629550750263
>100	0.047739370809514604	2.048254389649231
>500	0.0018361296465197923	0.26979290753474505
>1k	0.001224086431013195	0.7941767693004198
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3551	0.5843574536430598	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1203	0.19796733785767415	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0724070063652341	0.0
2	0.0	0.0	0.0	0.25293083814401096	0.0
3	0.0	0.0	0.0	0.3758581875868061	0.0
4	0.0	0.0	0.0	0.7064619961953409	0.0
5	0.0	0.0	0.0	1.1226377214173342	0.0
6	0.0	0.0	0.0	1.8708983076507877	0.0
7	0.0	0.0	0.0	2.1945905383790048	0.0
8	0.0	0.0	0.0	3.094576715223244	0.0
9	0.0	0.0	0.0	3.441142977507751	0.0
10	0.0	0.0	0.0	4.002297276838315	0.0
11	0.0	0.0	0.0	4.511779303444599	0.0
12	0.0	0.0	0.0	4.954120287784938	0.0
13	0.0	0.0	0.0	5.158340958010519	0.0
14	0.0	0.0	0.0	5.237330419499865	0.0
15	0.0	0.0	0.0	5.319611108551268	0.0
16	0.0	0.0	0.0	5.505565465807437	0.0
17	0.0	0.0	0.0	5.710608942923532	0.0
18	0.0	0.0	0.0	6.004351002837038	0.0
19	0.0	0.0	0.0	6.133202561891534	0.0
20	0.0	0.0	0.0	6.263699734727059	0.0
21	0.0	0.0	0.0	6.441261461699985	0.0
22	0.0	0.0	0.0	6.636595817508014	0.0
23	0.0	0.0	0.0	6.865007010314708	0.0
24	0.0	0.0	0.0	7.038783825591269	0.0
25	0.0	0.0	0.0	7.165331525352326	0.0
26	0.0	0.0	0.0	7.299284487128009	0.0
27	0.0	0.0	0.0	7.419743415899262	0.0
28	0.0	0.0	0.0	7.5382276081332815	0.0
29	0.0	0.0	0.0	7.677940218142563	0.0
30	0.0	0.0	0.0	7.870312469144742	0.0
31	0.0	0.0	0.0	8.044582968555613	0.0
32	0.0	0.0	0.0	8.190384349554696	0.0
33	0.0	0.0	0.0	8.336514853309987	0.0
34	0.0	0.0	0.0	8.478695883990811	0.0
35	0.0	0.0	0.0	8.680612694922953	0.0
36	0.0	0.0	0.0	8.833161092424252	0.0
37	0.0	0.0	0.0	8.992291945049665	0.0
38	0.0	0.0	0.0	9.142536483257526	0.0
39	0.0	0.0	0.0	9.301996458639143	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTAGG	35	1.2106648E-7	45.000004	2
GCTACGA	35	1.2106648E-7	45.000004	10
CGGGTCA	25	3.8886283E-5	45.0	6
AGATCTA	20	7.0306583E-4	45.0	13
CGCATCG	20	7.0306583E-4	45.0	21
GTAGGAC	20	7.0306583E-4	45.0	14
TAACGCA	20	7.0306583E-4	45.0	13
GACTTAG	20	7.0306583E-4	45.0	1
CGTAGTG	20	7.0306583E-4	45.0	33
TATAGCG	20	7.0306583E-4	45.0	1
TAATGCG	50	2.1827873E-11	45.0	1
ATACCGG	25	3.8886283E-5	45.0	2
CGGACCG	20	7.0306583E-4	45.0	30
ATTCGCA	30	2.1638061E-6	44.999996	14
CGAATAT	30	2.1638061E-6	44.999996	14
TAGTGCG	30	2.1638061E-6	44.999996	1
ATAACGG	75	0.0	42.0	2
CGTATGG	65	0.0	41.53846	2
TAGGGCG	180	0.0	41.25	5
AATGCGG	120	0.0	41.249996	2
>>END_MODULE