FastQCFastQC Report
Thu 26 May 2016
SRR1547086_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547086_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences982887
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65570.6671163623081798No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACGA453.8380676E-1045.024
ATCGGAT207.0327875E-445.011
TCGACTA207.0327875E-445.021
TATACGG650.041.538462
CGTTTTT38050.040.5650441
CGTTTAT850.039.70588339
AGGGCGA12400.039.0120966
GAACGTA356.2473664E-638.57142610
CACGACC9700.038.50515727
TAACGCC950.037.89473712
AACGAGC3750.037.815
ACGACCA10000.037.57528
CGTTAGG1500.037.5000042
CATACGA900.037.518
ACGGGAT2400.037.4999965
TACGATG301.1398016E-437.4999961
CGGTCTA601.546141E-1037.49999631
TATACGT301.1398016E-437.49999610
GGGCGAT26700.037.415737
GACACGA10000.037.35000225