##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547086_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	982887
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.459214538395564	31.0	31.0	33.0	30.0	34.0
2	31.83377438098174	31.0	31.0	34.0	30.0	34.0
3	31.926853239487347	33.0	31.0	34.0	30.0	34.0
4	35.68091347225062	37.0	35.0	37.0	33.0	37.0
5	35.617751582837094	37.0	35.0	37.0	33.0	37.0
6	35.65539070106737	37.0	35.0	37.0	33.0	37.0
7	36.01324669061652	37.0	35.0	37.0	35.0	37.0
8	34.68779015288634	37.0	35.0	37.0	33.0	37.0
9	36.990104661064805	39.0	37.0	39.0	33.0	39.0
10	36.90757330191568	39.0	37.0	39.0	32.0	39.0
11	36.81732488068313	39.0	37.0	39.0	32.0	39.0
12	36.46507380807763	39.0	35.0	39.0	32.0	39.0
13	36.355852707381416	39.0	35.0	39.0	32.0	39.0
14	37.377043342724036	40.0	35.0	41.0	32.0	41.0
15	37.57324188843682	40.0	35.0	41.0	32.0	41.0
16	37.63130858379448	40.0	35.0	41.0	33.0	41.0
17	37.52001908662949	39.0	35.0	41.0	32.0	41.0
18	37.44857343723134	39.0	36.0	41.0	32.0	41.0
19	37.39751670334433	39.0	35.0	41.0	32.0	41.0
20	37.26768387413813	39.0	35.0	41.0	32.0	41.0
21	37.14893268503907	39.0	35.0	41.0	32.0	41.0
22	37.05500225356526	39.0	35.0	41.0	32.0	41.0
23	36.977756344320355	39.0	35.0	41.0	32.0	41.0
24	36.82647038774549	38.0	35.0	41.0	31.0	41.0
25	36.72636528919398	38.0	35.0	41.0	31.0	41.0
26	36.612404070864706	38.0	35.0	41.0	31.0	41.0
27	36.504132214588246	38.0	35.0	41.0	31.0	41.0
28	36.52420064564899	38.0	35.0	41.0	31.0	41.0
29	36.477875890107406	38.0	35.0	41.0	31.0	41.0
30	36.40725129134885	38.0	35.0	40.0	31.0	41.0
31	36.293108973869835	38.0	35.0	40.0	30.0	41.0
32	36.2167766996613	38.0	35.0	41.0	30.0	41.0
33	36.081242299470844	38.0	35.0	41.0	30.0	41.0
34	35.90551100991264	38.0	35.0	41.0	29.0	41.0
35	35.77124023412661	38.0	35.0	41.0	29.0	41.0
36	35.64265678557148	38.0	35.0	40.0	28.0	41.0
37	35.58773694229347	38.0	35.0	40.0	28.0	41.0
38	35.46039575251275	38.0	34.0	40.0	27.0	41.0
39	35.38565165680287	38.0	34.0	40.0	27.0	41.0
40	35.231150681614466	38.0	34.0	40.0	27.0	41.0
41	35.13397877884233	37.0	34.0	40.0	26.0	41.0
42	35.04886014363808	37.0	34.0	40.0	26.0	41.0
43	34.95212471016505	37.0	34.0	40.0	26.0	41.0
44	34.82119307712891	37.0	34.0	40.0	26.0	41.0
45	34.68235005651718	36.0	34.0	40.0	25.0	41.0
46	34.60704638478279	36.0	34.0	40.0	25.0	41.0
47	34.526875419046135	36.0	34.0	40.0	25.0	41.0
48	34.44223700181201	36.0	34.0	40.0	24.0	41.0
49	34.355505770246225	36.0	34.0	40.0	24.0	41.0
50	34.262358745206726	35.0	34.0	40.0	24.0	41.0
51	33.895021502980505	35.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	4.0
12	3.0
13	7.0
14	15.0
15	32.0
16	103.0
17	199.0
18	486.0
19	866.0
20	1607.0
21	2389.0
22	3613.0
23	5155.0
24	7666.0
25	10745.0
26	13897.0
27	16450.0
28	18220.0
29	20276.0
30	23752.0
31	28022.0
32	34910.0
33	45355.0
34	75450.0
35	110256.0
36	80017.0
37	103646.0
38	145566.0
39	234003.0
40	175.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.67854086990671	23.720224196677748	27.487493475852258	15.113741457563282
2	33.490726807862956	25.553293511868606	26.931071425301177	14.024908254967256
3	29.56260485691641	25.692882294709364	30.743005045340922	14.00150780303331
4	25.873879703363663	28.368571361712995	29.88756591551216	15.869983019411183
5	23.79653001820148	31.732640679956088	29.207426692997263	15.263402608845167
6	21.95471096880923	39.99961338383761	26.99313349347382	11.052542153879337
7	86.93013540722382	3.83930197469292	7.3984089727506825	1.8321536453325766
8	84.57035244132845	6.018189273029352	6.988494099525175	2.4229641861170204
9	82.11788333755558	5.048189669819624	9.46650021823465	3.3674267743901387
10	43.644081160906595	27.507129507257698	15.402279204018365	13.446510127817337
11	36.27344750719055	25.735511813667287	22.337766192858385	15.653274486283774
12	31.162076617149275	23.249162925137885	26.95660844023779	18.63215201747505
13	24.078454593457842	29.411926294680875	28.96039931345109	17.549219798410192
14	20.790080650166296	30.663748732051598	30.512154499957777	18.03401611782433
15	20.12123468923691	23.067758552102124	37.90730775765678	18.903699001004185
16	23.585213763128415	21.93161574016138	35.439577489579165	19.043593007131033
17	23.18038594467116	22.04332746287213	28.893351931605565	25.88293466085115
18	25.717300157596956	22.628643984506866	29.959700352125928	21.694355505770247
19	28.578666723641682	24.808548693796947	25.79370772021606	20.819076862345316
20	29.994495806740755	24.619106774227355	25.18020891516522	20.20618850386667
21	27.04827716716164	25.706312119297536	27.357264873785088	19.88814583975574
22	26.351554146102245	22.977005495036558	26.821191042307003	23.85024931655419
23	24.385000513792534	26.87185810779876	26.380448617185902	22.362692761222807
24	23.083426680788328	25.140224664686784	31.321810136872298	20.45453851765259
25	23.743421166420962	25.119876445613787	29.250259694145917	21.88644269381933
26	21.46014750424006	28.349851000165838	26.85741087225693	23.33259062333717
27	22.682566765050304	26.390317503436307	27.88255414915448	23.04456158235891
28	21.523735688843175	26.595020587310646	30.732830935804422	21.14841278804176
29	22.27000662334531	24.85941924147944	30.60636675426575	22.264207380909504
30	24.435871061475023	25.268214962655932	29.739329139565385	20.556584836303664
31	27.556066974128257	24.94691658349332	26.552492809448086	20.944523632930338
32	27.995791988295704	25.0710407198386	26.402221211594007	20.53094608027169
33	26.802877645141304	25.1413438167358	25.724422034272504	22.33135650385039
34	22.82500429856128	27.052346810976236	28.86242263861461	21.26022625184787
35	23.379493268300426	26.809694298530758	28.774721814409997	21.03609061875882
36	27.013786935832908	26.84184448466609	25.360087171770505	20.784281407730493
37	24.637725394679144	27.73136688144212	26.521868739743226	21.109038984135513
38	24.273187049986415	28.59809927285639	25.645267462078554	21.48344621507864
39	24.433734498472358	26.56999227785086	25.478819030061437	23.51745419361534
40	25.768577669660907	25.40994030849935	25.832267595359387	22.98921442648036
41	22.30744734643962	25.212257360205193	27.062724402703463	25.417570890651724
42	24.66560245480915	26.113581724043556	25.851700144574096	23.369115676573195
43	24.07469017292934	25.213579994444935	26.954675359425856	23.757054473199872
44	23.833563776914335	26.87185810779876	27.247791455172365	22.046786660114538
45	22.453751041574463	27.83900896033827	25.446974067212203	24.260265930875065
46	24.046100925131782	26.78771822193192	26.724537001710267	22.441643851226033
47	23.726735626781107	26.195178082526272	28.187268729772597	21.890817560920027
48	24.524182332251826	25.71902975621816	27.85884847393444	21.897939437595575
49	23.982919704910127	25.049776830907316	28.226439051488118	22.74086441269444
50	22.012601652071908	27.763618808672817	27.528291655093618	22.695487884161658
51	22.754701201664076	28.01288449231702	25.91945971408717	23.312954591931728
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	976.0
1	1309.0
2	1642.0
3	6262.5
4	10883.0
5	7474.5
6	4066.0
7	4144.0
8	4222.0
9	4397.5
10	4573.0
11	4831.5
12	5090.0
13	5054.0
14	5018.0
15	4857.0
16	4696.0
17	4669.0
18	4642.0
19	4562.0
20	4482.0
21	4590.5
22	4699.0
23	5009.0
24	5319.0
25	5610.5
26	6905.0
27	7908.0
28	8921.5
29	9935.0
30	11243.0
31	12551.0
32	15062.0
33	17573.0
34	19097.0
35	20621.0
36	22620.5
37	24620.0
38	27853.0
39	31086.0
40	37417.5
41	43749.0
42	52008.5
43	60268.0
44	62041.5
45	63815.0
46	66399.0
47	68983.0
48	71335.0
49	73687.0
50	74035.0
51	74383.0
52	71367.5
53	68352.0
54	64586.0
55	60820.0
56	57140.5
57	53461.0
58	50240.5
59	47020.0
60	44720.5
61	42421.0
62	38666.0
63	34911.0
64	31987.5
65	29064.0
66	25721.0
67	22378.0
68	19014.0
69	15650.0
70	13653.0
71	11656.0
72	10046.5
73	8437.0
74	7257.5
75	4906.5
76	3735.0
77	2859.5
78	1984.0
79	1369.5
80	755.0
81	573.5
82	392.0
83	286.5
84	181.0
85	141.5
86	102.0
87	80.0
88	58.0
89	39.0
90	20.0
91	18.0
92	16.0
93	10.5
94	5.0
95	3.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	982887.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.410700173596975
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.15622954984738	21.35241103918743
2	6.113274801670678	3.473648349381423
3	2.616465660617058	2.2300686419491074
4	1.7056203880759648	1.938314778224014
5	1.265723551773494	1.7980046166048491
6	1.0460378319808223	1.7831200328787933
7	0.8814424508829487	1.752967803462115
8	0.7799915109945091	1.7728083965452692
9	0.69083423226281	1.7664375818228175
>10	9.514709821258608	56.401258886975526
>50	0.19976138635646828	3.452843281615863
>100	0.028801080417064084	1.4588086811269034
>500	7.384892414631539E-4	0.13144594257528908
>1k	0.0	0.0
>5k	3.6924462073157694E-4	0.687861967650575
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6557	0.6671163623081798	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.018415138261061545	0.0
2	0.0	0.0	0.0	0.08780256529997853	0.0
3	0.0	0.0	0.0	0.16766932516148855	0.0
4	0.0	0.0	0.0	0.3165165476804556	0.0
5	0.0	0.0	0.0	0.53353030409396	0.0
6	0.0	0.0	0.0	1.0711302520025192	0.0
7	0.0	0.0	0.0	1.3268056246547162	0.0
8	0.0	0.0	0.0	1.8858729436852864	0.0
9	0.0	0.0	0.0	2.228435211779177	0.0
10	0.0	0.0	0.0	2.7214725599178746	0.0
11	0.0	0.0	0.0	3.107986981209437	0.0
12	0.0	0.0	0.0	3.4094458467758755	0.0
13	0.0	0.0	0.0	3.5865770938063073	0.0
14	0.0	0.0	0.0	3.673769212534096	0.0
15	0.0	0.0	0.0	3.748345435436627	0.0
16	0.0	0.0	0.0	3.8687051512533994	0.0
17	1.0174109536498091E-4	0.0	0.0	3.991404912263566	0.0
18	1.0174109536498091E-4	0.0	0.0	4.153885441561441	0.0
19	1.0174109536498091E-4	0.0	0.0	4.236600952093171	0.0
20	1.0174109536498091E-4	0.0	0.0	4.329795795447493	0.0
21	1.0174109536498091E-4	0.0	0.0	4.439777919537037	0.0
22	1.0174109536498091E-4	0.0	0.0	4.55952718878162	0.0
23	1.0174109536498091E-4	0.0	0.0	4.693214988091205	0.0
24	1.0174109536498091E-4	0.0	0.0	4.8119468463821375	0.0
25	1.0174109536498091E-4	0.0	0.0	4.90819392259741	0.0
26	1.0174109536498091E-4	0.0	0.0	5.007798454959726	0.0
27	1.0174109536498091E-4	0.0	0.0	5.113507453043941	0.0
28	1.0174109536498091E-4	0.0	0.0	5.217995557983777	0.0
29	1.0174109536498091E-4	0.0	0.0	5.3293003163130654	0.0
30	1.0174109536498091E-4	0.0	0.0	5.485167674412216	0.0
31	1.0174109536498091E-4	0.0	0.0	5.607053506659463	0.0
32	1.0174109536498091E-4	0.0	0.0	5.739927377206128	0.0
33	1.0174109536498091E-4	0.0	0.0	5.859167940973886	0.0
34	1.0174109536498091E-4	0.0	0.0	5.985123417035733	0.0
35	1.0174109536498091E-4	0.0	0.0	6.121863449206267	0.0
36	1.0174109536498091E-4	0.0	0.0	6.244563210216434	0.0
37	1.0174109536498091E-4	0.0	0.0	6.382320653340618	0.0
38	1.0174109536498091E-4	0.0	0.0	6.550193460692837	0.0
39	1.0174109536498091E-4	0.0	0.0	6.828048392134599	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCACGA	45	3.8380676E-10	45.0	24
ATCGGAT	20	7.0327875E-4	45.0	11
TCGACTA	20	7.0327875E-4	45.0	21
TATACGG	65	0.0	41.53846	2
CGTTTTT	3805	0.0	40.565044	1
CGTTTAT	85	0.0	39.705883	39
AGGGCGA	1240	0.0	39.012096	6
GAACGTA	35	6.2473664E-6	38.571426	10
CACGACC	970	0.0	38.505157	27
TAACGCC	95	0.0	37.894737	12
AACGAGC	375	0.0	37.8	15
ACGACCA	1000	0.0	37.575	28
CGTTAGG	150	0.0	37.500004	2
CATACGA	90	0.0	37.5	18
ACGGGAT	240	0.0	37.499996	5
TACGATG	30	1.1398016E-4	37.499996	1
CGGTCTA	60	1.546141E-10	37.499996	31
TATACGT	30	1.1398016E-4	37.499996	10
GGGCGAT	2670	0.0	37.41573	7
GACACGA	1000	0.0	37.350002	25
>>END_MODULE