FastQCFastQC Report
Thu 26 May 2016
SRR1547085_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547085_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences894616
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61350.6857690897547104No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACCG406.8121153E-945.01
TATCGTC207.032448E-445.013
TGTAACG207.032448E-445.01
TATAGCG800.042.18751
CGTAAGG1200.041.2500042
CGGTCTA850.039.70588331
CGTTTTT40450.039.548831
CGAATAT750.039.014
TAACGGG2150.038.7209323
GCGTTAG700.038.571431
CATACGA1400.038.5714318
GCGATAT1400.038.571439
GCGATAC659.094947E-1238.0769279
AGGGCGA10400.038.0769276
GCGGGAT2900.038.017245
AGTACGG1250.037.82
GGGCGAT21950.037.722097
ACACGAC4600.037.6630426
ATTACGA301.1397194E-437.50000440
TATTCGG601.546141E-1037.5000042
Produced by FastQC (version 0.11.5)