##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547085_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	894616
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.495243769393795	31.0	31.0	33.0	30.0	34.0
2	31.86330671483631	31.0	31.0	34.0	30.0	34.0
3	31.954434081214732	33.0	31.0	34.0	30.0	34.0
4	35.72031687338478	37.0	35.0	37.0	33.0	37.0
5	35.66272009443158	37.0	35.0	37.0	33.0	37.0
6	35.71455015336189	37.0	35.0	37.0	33.0	37.0
7	36.03773462580593	37.0	35.0	37.0	35.0	37.0
8	34.725184883793716	37.0	35.0	37.0	33.0	37.0
9	37.03660900319243	39.0	37.0	39.0	34.0	39.0
10	36.96145161723019	39.0	37.0	39.0	32.0	39.0
11	36.88312192046644	39.0	37.0	39.0	33.0	39.0
12	36.57727896661808	39.0	35.0	39.0	32.0	39.0
13	36.49241462258667	39.0	35.0	39.0	32.0	39.0
14	37.55886659751223	40.0	36.0	41.0	32.0	41.0
15	37.73297817164012	40.0	36.0	41.0	33.0	41.0
16	37.77869722875513	40.0	36.0	41.0	33.0	41.0
17	37.68697519382618	40.0	35.0	41.0	33.0	41.0
18	37.606117037924655	39.0	36.0	41.0	33.0	41.0
19	37.531225687892906	39.0	36.0	41.0	33.0	41.0
20	37.38769594999419	39.0	35.0	41.0	32.0	41.0
21	37.294898593362966	39.0	35.0	41.0	32.0	41.0
22	37.21866365010239	39.0	35.0	41.0	32.0	41.0
23	37.13211590224186	39.0	35.0	41.0	32.0	41.0
24	36.98120646176684	39.0	35.0	41.0	32.0	41.0
25	36.87486698203475	38.0	35.0	41.0	31.0	41.0
26	36.77328932189901	38.0	35.0	41.0	31.0	41.0
27	36.66270556305722	38.0	35.0	41.0	31.0	41.0
28	36.69138378924589	38.0	35.0	41.0	31.0	41.0
29	36.61824626431899	38.0	35.0	41.0	31.0	41.0
30	36.54349352124263	38.0	35.0	41.0	31.0	41.0
31	36.42009979700788	38.0	35.0	41.0	31.0	41.0
32	36.324721444731594	38.0	35.0	41.0	30.0	41.0
33	36.148705142765166	38.0	35.0	41.0	30.0	41.0
34	35.94044148550886	38.0	35.0	41.0	29.0	41.0
35	35.76560110706717	38.0	35.0	41.0	29.0	41.0
36	35.62069983098894	38.0	35.0	41.0	28.0	41.0
37	35.541273574360396	38.0	35.0	41.0	27.0	41.0
38	35.433435127473686	38.0	35.0	40.0	27.0	41.0
39	35.32745669650442	38.0	35.0	40.0	27.0	41.0
40	35.173459897877976	38.0	34.0	40.0	26.0	41.0
41	35.073510869468016	38.0	34.0	40.0	25.0	41.0
42	35.0154099636045	38.0	34.0	40.0	25.0	41.0
43	34.91374511522262	37.0	34.0	40.0	25.0	41.0
44	34.786163001779535	37.0	34.0	40.0	24.0	41.0
45	34.65587469931233	37.0	34.0	40.0	24.0	41.0
46	34.58833734250226	37.0	34.0	40.0	24.0	41.0
47	34.493287622846005	36.0	34.0	40.0	23.0	41.0
48	34.41635629141442	36.0	34.0	40.0	23.0	41.0
49	34.333963398821396	36.0	34.0	40.0	24.0	41.0
50	34.21180372360879	36.0	34.0	40.0	24.0	41.0
51	33.84552366601984	35.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	3.0
12	1.0
13	5.0
14	11.0
15	37.0
16	79.0
17	166.0
18	379.0
19	728.0
20	1302.0
21	2106.0
22	3277.0
23	4788.0
24	7077.0
25	10362.0
26	13459.0
27	15300.0
28	16316.0
29	18029.0
30	20532.0
31	24713.0
32	30543.0
33	39954.0
34	66182.0
35	96252.0
36	71994.0
37	94289.0
38	134261.0
39	222316.0
40	154.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.07605497777818	23.524506603950744	26.550609423484488	14.84882899478659
2	33.88381160184928	25.061926010712977	27.789912096363135	13.264350291074607
3	29.67664338665975	25.56549402201615	31.082945084818515	13.674917506505585
4	25.623954858844467	28.711983689091188	30.712395038765234	14.951666413299114
5	23.548874600946103	31.987243688912336	29.829669936598496	14.634211773543063
6	21.776829388251496	40.595629856832424	26.927866257701627	10.699674497214447
7	86.1365099662872	3.962705786616828	8.146064903824659	1.754719343271303
8	83.55529076162287	6.2136156742110575	7.532170227225983	2.6989233369400947
9	80.79332361594248	5.400305829540272	10.389709104241373	3.416661450275873
10	42.969944646641686	26.106284707628745	17.379635508419256	13.54413513731031
11	34.661128350040684	25.649440653867135	24.129235336725475	15.560195659366698
12	30.086763482879807	23.193638387866976	28.18863065270462	18.5309674765486
13	23.4947731764243	28.63519096461499	30.15003085122555	17.72000500773516
14	20.363150223112488	29.810555590331493	31.261233870174465	18.565060316381555
15	19.780665671081223	23.94133348833466	37.70746331386874	18.570537526715373
16	22.563759199477765	22.336622640328365	35.76227118674381	19.33734697345006
17	22.47344111887111	22.894627415561537	29.978784193441655	24.653147272125693
18	24.187137274540138	23.228960805529972	31.627089164513045	20.95681275541685
19	27.08916451304247	25.631891224838366	26.897238591753332	20.381705670365832
20	28.302087152476595	25.297334275264472	26.425974943439417	19.974603628819516
21	25.58360235005857	26.72353277830935	28.406042369016426	19.286822502615646
22	25.140842551441068	23.56709470879126	27.80980890124925	23.482253838518428
23	23.051119139384944	27.279749076698828	27.251021667396962	22.418110116519266
24	22.248875495184524	25.304599962441987	32.399822940792475	20.046701601581013
25	22.991428724726585	25.614230016006868	29.624330438981637	21.770010820284906
26	21.402367049102633	28.026214599336473	28.256592772765078	22.31482557879582
27	21.594404750194496	26.865269568172266	28.63206113013852	22.90826455149472
28	20.839108623141104	27.241967503375754	30.713736396398005	21.20518747708514
29	21.879555026961288	24.883972564765216	30.843065628157778	22.393406780115715
30	23.53601992363204	26.023455873805073	30.178981820132883	20.261542382430004
31	25.476293739436805	26.704418432042353	27.049818022481155	20.769469806039687
32	27.561881298791885	24.7952194013968	27.12638718735189	20.516512112459424
33	25.668331440528675	26.04324089888846	25.926766344442754	22.36166131614011
34	23.291892834467525	26.42262154935749	28.654193531079258	21.631292085095726
35	22.35719012403087	27.654546755255886	28.43868207141388	21.549581049299366
36	26.531271517612026	27.643145215377324	25.206680855249626	20.618902411761024
37	23.316372611265614	29.020495944628756	26.17804734098205	21.485084103123576
38	23.951058331172256	29.064984306115697	25.51541667039266	21.468540692319387
39	22.81671689305803	28.15599095030717	25.40721382134905	23.620078335285754
40	24.981332772943922	25.727462956173376	26.80658517173849	22.484619099144215
41	21.167629463367525	25.476070179831346	27.420032729126238	25.93626762767489
42	23.30217657631878	26.518975739311617	26.157591637082277	24.021256047287327
43	22.81246926055425	25.365743514535843	27.994245575755407	23.827541649154497
44	23.14099010078067	26.949998658642365	27.409301868064063	22.4997093725129
45	21.718703890831375	27.75067738560455	26.363042914501868	24.16757580906221
46	23.282726890643584	27.015166283634546	27.352070609065787	22.350036216656086
47	22.88378477469663	26.1407128868699	28.17119300347859	22.804309334954887
48	23.26215940694108	25.349088323928925	28.76329061854472	22.62546165058528
49	23.219571302100565	25.14944959625135	28.4809348368462	23.15004426480188
50	21.980827528235576	27.38649878830694	28.35831239325029	22.274361290207196
51	21.678686721453673	27.562216638200077	27.173893603512568	23.58520303683368
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1086.0
1	1392.0
2	1698.0
3	6190.5
4	10683.0
5	7567.0
6	4451.0
7	4547.0
8	4643.0
9	4990.0
10	5337.0
11	5431.5
12	5526.0
13	5599.5
14	5673.0
15	5560.0
16	5447.0
17	5238.5
18	5030.0
19	5011.0
20	4992.0
21	4896.0
22	4800.0
23	5017.0
24	5234.0
25	5381.5
26	6327.0
27	7125.0
28	8533.5
29	9942.0
30	11632.5
31	13323.0
32	14955.5
33	16588.0
34	18899.0
35	21210.0
36	23863.0
37	26516.0
38	28881.0
39	31246.0
40	36514.0
41	41782.0
42	47348.5
43	52915.0
44	55212.5
45	57510.0
46	60742.0
47	63974.0
48	66606.5
49	69239.0
50	68048.0
51	66857.0
52	62583.0
53	58309.0
54	53760.5
55	49212.0
56	47227.5
57	45243.0
58	42991.0
59	40739.0
60	38572.0
61	36405.0
62	33367.5
63	30330.0
64	27148.0
65	23966.0
66	21503.0
67	19040.0
68	16276.0
69	13512.0
70	12100.0
71	10688.0
72	8968.5
73	7249.0
74	6281.0
75	4271.5
76	3230.0
77	2284.0
78	1338.0
79	1086.0
80	834.0
81	667.0
82	500.0
83	365.5
84	231.0
85	151.0
86	71.0
87	44.5
88	18.0
89	16.0
90	14.0
91	15.5
92	17.0
93	9.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	894616.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.884284045078633
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.03286818800751	21.383863934142745
2	6.265524330303114	3.6195036889565246
3	2.807702277752523	2.4329541031385444
4	1.8969859802862359	2.191723275364783
5	1.4496928937852194	2.0936670661112142
6	1.1868886715411422	2.0569457711208234
7	0.9679947587869155	1.9571884896864054
8	0.8514679466260715	1.9675233620501842
9	0.7853622485279057	2.0416163638285503
>10	9.602116593700332	55.826348694313154
>50	0.1294902240791005	2.3092770211894
>100	0.021913729386871143	1.1145892163605544
>500	0.0015937257735906245	0.29875824529395933
>1k	0.0	0.0
>5k	3.9843144339765614E-4	0.7060407684431588
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6135	0.6857690897547104	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1177980273100415E-4	0.0	0.0	0.0325279225947222	0.0
2	1.1177980273100415E-4	0.0	0.0	0.08864138356568628	0.0
3	1.1177980273100415E-4	0.0	0.0	0.1561563844152128	0.0
4	1.1177980273100415E-4	0.0	0.0	0.3266205835799941	0.0
5	1.1177980273100415E-4	0.0	0.0	0.5343074570541998	0.0
6	1.1177980273100415E-4	0.0	0.0	1.2164995931215181	0.0
7	1.1177980273100415E-4	0.0	0.0	1.5260178668836686	0.0
8	1.1177980273100415E-4	0.0	0.0	2.321107603709301	0.0
9	1.1177980273100415E-4	0.0	0.0	2.7875647205057814	0.0
10	1.1177980273100415E-4	0.0	0.0	3.377538519320021	0.0
11	1.1177980273100415E-4	0.0	0.0	3.78151072638987	0.0
12	1.1177980273100415E-4	0.0	0.0	4.118191492215654	0.0
13	1.1177980273100415E-4	0.0	0.0	4.3144768258112975	0.0
14	1.1177980273100415E-4	0.0	0.0	4.423462133474027	0.0
15	1.1177980273100415E-4	0.0	0.0	4.4986899407119925	0.0
16	1.1177980273100415E-4	0.0	0.0	4.61304067890581	0.0
17	1.1177980273100415E-4	0.0	0.0	4.727950316113282	0.0
18	1.1177980273100415E-4	0.0	0.0	4.893496203957899	0.0
19	1.1177980273100415E-4	0.0	0.0	4.9870558988437494	0.0
20	1.1177980273100415E-4	0.0	0.0	5.076591520831284	0.0
21	1.1177980273100415E-4	0.0	0.0	5.189489121589598	0.0
22	1.1177980273100415E-4	0.0	0.0	5.320047931179411	0.0
23	1.1177980273100415E-4	0.0	0.0	5.454966153075733	0.0
24	1.1177980273100415E-4	0.0	0.0	5.572223166140557	0.0
25	1.1177980273100415E-4	0.0	0.0	5.675507703864004	0.0
26	1.1177980273100415E-4	0.0	0.0	5.779239360798376	0.0
27	1.1177980273100415E-4	0.0	0.0	5.879282284242625	0.0
28	1.1177980273100415E-4	0.0	0.0	5.981784363346955	0.0
29	1.1177980273100415E-4	0.0	0.0	6.099935614833627	0.0
30	1.1177980273100415E-4	0.0	0.0	6.263134126820893	0.0
31	1.1177980273100415E-4	0.0	0.0	6.389109964498735	0.0
32	1.1177980273100415E-4	0.0	0.0	6.530064295742531	0.0
33	1.1177980273100415E-4	0.0	0.0	6.660958444740537	0.0
34	1.1177980273100415E-4	0.0	0.0	6.803254133617105	0.0
35	1.1177980273100415E-4	0.0	0.0	6.964552388957944	0.0
36	1.1177980273100415E-4	0.0	0.0	7.097458574405108	0.0
37	1.1177980273100415E-4	0.0	0.0	7.234388832750588	0.0
38	1.1177980273100415E-4	0.0	0.0	7.4074239673781825	0.0
39	1.1177980273100415E-4	0.0	0.0	7.70665849928908	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACCG	40	6.8121153E-9	45.0	1
TATCGTC	20	7.032448E-4	45.0	13
TGTAACG	20	7.032448E-4	45.0	1
TATAGCG	80	0.0	42.1875	1
CGTAAGG	120	0.0	41.250004	2
CGGTCTA	85	0.0	39.705883	31
CGTTTTT	4045	0.0	39.54883	1
CGAATAT	75	0.0	39.0	14
TAACGGG	215	0.0	38.720932	3
GCGTTAG	70	0.0	38.57143	1
CATACGA	140	0.0	38.57143	18
GCGATAT	140	0.0	38.57143	9
GCGATAC	65	9.094947E-12	38.076927	9
AGGGCGA	1040	0.0	38.076927	6
GCGGGAT	290	0.0	38.01724	5
AGTACGG	125	0.0	37.8	2
GGGCGAT	2195	0.0	37.72209	7
ACACGAC	460	0.0	37.66304	26
ATTACGA	30	1.1397194E-4	37.500004	40
TATTCGG	60	1.546141E-10	37.500004	2
>>END_MODULE