FastQCFastQC Report
Thu 26 May 2016
SRR1547084_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547084_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1322000
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104070.787216338880484No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT71400.042.1953771
GCGATAC1250.041.49
TGTAGCG900.040.01
GGCGATA6050.038.677698
TTTAGCG1050.038.571431
TAGGGTG14000.038.4107135
TATGGGT11650.038.047214
AGGGTAC10350.038.043486
GCGATAA950.037.8947379
TATACGG950.037.8947372
ATAGGGT12000.037.8754
TCGTTGA1250.037.80000324
CGACGGT601.5643309E-1037.49999628
ATTCGCG301.14001465E-437.4999961
ATAACGC601.5643309E-1037.49999611
CGATCAA301.14001465E-437.49999610
TATAACG301.14001465E-437.4999961
GTACGCA301.14001465E-437.4999969
ACGGGTA4150.037.4096375
GGGCGAT29000.037.086217
Produced by FastQC (version 0.11.5)