##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547084_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1322000
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.42759682299546	31.0	31.0	33.0	30.0	34.0
2	31.799399394856277	31.0	31.0	34.0	30.0	34.0
3	31.898812405446293	31.0	31.0	34.0	30.0	34.0
4	35.6832602118003	37.0	35.0	37.0	33.0	37.0
5	35.632560514372166	37.0	35.0	37.0	33.0	37.0
6	35.679714069591526	37.0	35.0	37.0	33.0	37.0
7	36.04207488653555	37.0	35.0	37.0	35.0	37.0
8	34.74052042360061	37.0	35.0	37.0	33.0	37.0
9	37.043247352496216	39.0	37.0	39.0	34.0	39.0
10	36.70087291981846	39.0	37.0	39.0	32.0	39.0
11	36.79554765506808	39.0	37.0	39.0	32.0	39.0
12	36.66778063540091	39.0	35.0	39.0	32.0	39.0
13	36.63150226928896	39.0	35.0	39.0	32.0	39.0
14	37.76811497730711	40.0	36.0	41.0	33.0	41.0
15	37.89729273827534	40.0	36.0	41.0	33.0	41.0
16	37.906920574886534	40.0	36.0	41.0	33.0	41.0
17	37.80246293494705	40.0	36.0	41.0	33.0	41.0
18	37.709773827534036	39.0	36.0	41.0	33.0	41.0
19	37.63989258698941	39.0	36.0	41.0	33.0	41.0
20	37.49379576399395	39.0	35.0	41.0	33.0	41.0
21	37.400605143721634	39.0	35.0	41.0	32.0	41.0
22	37.30218078668684	39.0	35.0	41.0	32.0	41.0
23	37.21473222390318	39.0	35.0	41.0	32.0	41.0
24	37.06824810892587	39.0	35.0	41.0	32.0	41.0
25	36.98582980332829	39.0	35.0	41.0	32.0	41.0
26	36.88313313161876	39.0	35.0	41.0	31.0	41.0
27	36.795505295007565	39.0	35.0	41.0	31.0	41.0
28	36.797528744326776	39.0	35.0	41.0	31.0	41.0
29	36.75511119515885	39.0	35.0	41.0	31.0	41.0
30	36.66969894099849	39.0	35.0	41.0	31.0	41.0
31	36.57439561270802	39.0	35.0	41.0	31.0	41.0
32	36.45475794251135	39.0	35.0	41.0	30.0	41.0
33	36.28746898638427	39.0	35.0	41.0	30.0	41.0
34	36.0901134644478	39.0	35.0	41.0	29.0	41.0
35	35.950153555219366	39.0	35.0	41.0	29.0	41.0
36	35.81111422087746	39.0	35.0	41.0	28.0	41.0
37	35.77340393343419	39.0	35.0	41.0	27.0	41.0
38	35.66813842662632	39.0	35.0	41.0	27.0	41.0
39	35.58921104387292	38.0	35.0	41.0	27.0	41.0
40	35.46399394856278	38.0	35.0	41.0	26.0	41.0
41	35.36495990922844	38.0	35.0	41.0	26.0	41.0
42	35.302519667170955	38.0	35.0	41.0	26.0	41.0
43	35.205595310136154	38.0	34.0	40.0	25.0	41.0
44	35.099054462934944	38.0	34.0	40.0	25.0	41.0
45	34.959804841149776	38.0	34.0	40.0	24.0	41.0
46	34.89136838124055	38.0	34.0	40.0	24.0	41.0
47	34.81265128593041	38.0	34.0	40.0	24.0	41.0
48	34.72502723146747	37.0	34.0	40.0	23.0	41.0
49	34.65556732223903	37.0	34.0	40.0	24.0	41.0
50	34.54527382753404	37.0	34.0	40.0	24.0	41.0
51	34.18228139183056	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	5.0
11	3.0
12	2.0
13	6.0
14	14.0
15	37.0
16	93.0
17	228.0
18	571.0
19	1129.0
20	1843.0
21	3058.0
22	4599.0
23	6975.0
24	10257.0
25	15562.0
26	20886.0
27	23319.0
28	24523.0
29	26269.0
30	30047.0
31	35280.0
32	42929.0
33	55211.0
34	86347.0
35	125366.0
36	102245.0
37	134885.0
38	203648.0
39	366458.0
40	205.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.898789712556734	23.109001512859304	27.140242057488656	15.851966717095308
2	33.53676248108926	25.267397881996974	27.96444780635401	13.231391830559758
3	28.556354009077157	25.329349470499245	32.54795763993948	13.566338880484116
4	25.067549167927382	27.887594553706506	32.27617246596067	14.768683812405447
5	22.57390317700454	32.08895612708018	30.839258698940995	14.497881996974282
6	20.762632375189106	40.812556732223904	28.12239031770045	10.302420574886535
7	86.73169440242057	3.362934947049925	8.388880484114978	1.5164901664145236
8	84.61157337367625	5.4366868381240545	7.747806354009078	2.20393343419062
9	81.58706505295008	4.021406959152799	11.513010590015128	2.878517397881997
10	34.552269288956126	21.196066565809378	30.78910741301059	13.462556732223904
11	32.44024205748865	26.03736762481089	24.875945537065054	16.6464447806354
12	27.915733736762483	24.54947049924357	28.529954614220877	19.00484114977307
13	22.907942511346445	28.26232980332829	30.284341906202723	18.54538577912254
14	20.805824508320725	29.6732980332829	31.10741301059001	18.413464447806355
15	20.84659606656581	24.809757942511347	36.14175491679274	18.201891074130106
16	22.19614220877458	23.16800302571861	35.49334341906203	19.142511346444778
17	22.422617246596065	23.17125567322239	30.862178517397883	23.54394856278366
18	23.731391830559758	23.543721633888048	32.419288956127076	20.305597579425115
19	26.120726172465965	25.89538577912254	28.129576399394857	19.854311649016644
20	27.25726172465961	25.2035552193646	27.99039334341906	19.548789712556733
21	24.938956127080182	27.08464447806354	29.310136157337368	18.66626323751891
22	24.3124810892587	23.25605143721634	29.87745839636914	22.554009077155822
23	22.40968229954614	27.26512859304085	28.906278366111955	21.418910741301058
24	21.526323751891073	25.561951588502268	33.327534039334346	19.584190620272317
25	22.07375189107413	26.150453857791224	31.05158850226929	20.724205748865355
26	20.802571860816943	28.798789712556733	29.155219364599095	21.243419062027233
27	21.46089258698941	27.327004538577913	29.64387291981846	21.56822995461422
28	20.154009077155823	27.35030257186082	32.19296520423601	20.302723146747354
29	21.167246596066565	25.47102874432678	31.679576399394854	21.6821482602118
30	22.61195158850227	26.34606656580938	30.71157337367625	20.330408472012103
31	24.32586989409985	26.23350983358548	28.072390317700453	21.368229954614222
32	25.0820726172466	26.160136157337366	27.9357034795764	20.82208774583964
33	23.57745839636914	26.294931921331315	27.429500756429654	22.698108925869896
34	21.12057488653555	27.893797276853256	29.151815431164902	21.83381240544629
35	21.380408472012103	26.92571860816944	29.207866868381238	22.486006051437215
36	23.67216338880484	28.236081694402422	27.27609682299546	20.815658093797275
37	21.5124810892587	29.33275340393343	27.362102874432676	21.79266263237519
38	21.934568835098336	29.168683812405444	27.87344931921331	21.023298033282906
39	21.32201210287443	28.608472012102876	27.23350983358548	22.836006051437217
40	23.32715582450832	26.257866868381242	29.005673222390314	21.40930408472012
41	20.349773071104387	26.604765506807865	28.914750378214826	24.13071104387292
42	22.702420574886535	27.536384266263237	26.896066565809377	22.865128593040847
43	21.844780635400905	26.500151285930407	28.657715582450834	22.99735249621785
44	22.017927382753406	27.912027231467473	28.283585476550684	21.78645990922844
45	20.84538577912254	28.456278366111952	27.71913767019667	22.979198184568837
46	22.62125567322239	27.263842662632378	28.083509833585474	22.03139183055976
47	22.10574886535552	27.034720121028744	28.817322239031768	22.042208774583962
48	22.42798789712557	26.540468986384262	29.088653555219363	21.9428895612708
49	22.048335854765508	26.182904689863847	28.88434190620272	22.884417549167928
50	21.39659606656581	27.598108925869898	29.105068078668683	21.900226928895613
51	20.69357034795764	28.195083207261725	27.955748865355524	23.155597579425113
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1341.0
1	1626.5
2	1912.0
3	9878.0
4	17844.0
5	12595.0
6	7346.0
7	7324.0
8	7302.0
9	7656.0
10	8010.0
11	8112.0
12	8214.0
13	8175.0
14	8136.0
15	7956.0
16	7776.0
17	7502.0
18	7228.0
19	7212.5
20	7197.0
21	7041.5
22	6886.0
23	7281.5
24	7677.0
25	8576.5
26	10872.0
27	12268.0
28	14160.0
29	16052.0
30	18444.5
31	20837.0
32	24193.5
33	27550.0
34	31147.0
35	34744.0
36	39522.0
37	44300.0
38	49162.5
39	54025.0
40	61809.0
41	69593.0
42	76898.0
43	84203.0
44	87886.5
45	91570.0
46	98548.5
47	105527.0
48	107863.5
49	110200.0
50	107659.5
51	105119.0
52	99361.5
53	93604.0
54	84660.0
55	75716.0
56	68764.5
57	61813.0
58	56763.5
59	51714.0
60	46595.5
61	41477.0
62	37285.0
63	33093.0
64	29458.0
65	25823.0
66	21410.5
67	16998.0
68	14859.5
69	12721.0
70	10978.5
71	9236.0
72	7847.5
73	6459.0
74	5567.0
75	3873.0
76	3071.0
77	2347.5
78	1624.0
79	1327.0
80	1030.0
81	709.5
82	389.0
83	258.5
84	128.0
85	89.0
86	50.0
87	40.0
88	30.0
89	21.5
90	13.0
91	7.0
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1322000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.47474481077904
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.93420474715116	21.337323577737966
2	6.375215108928255	3.630652466811099
3	2.8729065614593625	2.4541584360830413
4	1.8087560626480375	2.060154692354094
5	1.3972491915622987	1.9893157083401891
6	1.1635674695521836	1.9879372059373406
7	0.9289898191054212	1.8516923621587058
8	0.8021163511757375	1.8272046726625881
9	0.7107622539121762	1.8214896421155022
>10	8.67014194472519	53.30501692776352
>50	0.30990340327271637	5.3684045544846715
>100	0.024277611455836533	1.241153186820275
>500	0.00163669290140338	0.317143028430814
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	2.7278215023389667E-4	0.8083535383001423
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10407	0.787216338880484	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03207261724659607	0.0
2	0.0	0.0	0.0	0.10098335854765507	0.0
3	0.0	0.0	0.0	0.1848714069591528	0.0
4	0.0	0.0	0.0	0.3464447806354009	0.0
5	0.0	0.0	0.0	0.5710287443267776	0.0
6	0.0	0.0	0.0	1.2376701966717096	0.0
7	0.0	0.0	0.0	1.5444780635400908	0.0
8	0.0	0.0	0.0	2.339939485627837	0.0
9	0.0	0.0	0.0	2.745385779122542	0.0
10	0.0	0.0	0.0	3.2910741301059003	0.0
11	0.0	0.0	0.0	3.6411497730711044	0.0
12	0.0	0.0	0.0	3.9354765506807867	0.0
13	0.0	0.0	0.0	4.105219364599092	0.0
14	0.0	0.0	0.0	4.200075642965205	0.0
15	0.0	0.0	0.0	4.275642965204236	0.0
16	0.0	0.0	0.0	4.406656580937973	0.0
17	0.0	0.0	0.0	4.543721633888048	0.0
18	0.0	0.0	0.0	4.728366111951589	0.0
19	0.0	0.0	0.0	4.828971255673222	0.0
20	0.0	0.0	0.0	4.939712556732224	0.0
21	0.0	0.0	0.0	5.070272314674735	0.0
22	0.0	0.0	0.0	5.216717095310136	0.0
23	0.0	0.0	0.0	5.379425113464448	0.0
24	0.0	0.0	0.0	5.521482602118003	0.0
25	0.0	0.0	0.0	5.646822995461422	0.0
26	0.0	0.0	0.0	5.764599092284418	0.0
27	0.0	0.0	0.0	5.887443267776097	0.0
28	0.0	0.0	0.0	6.009909228441755	0.0
29	0.0	0.0	0.0	6.1470499243570345	0.0
30	0.0	0.0	0.0	6.331164901664145	0.0
31	0.0	0.0	0.0	6.4885779122541605	0.0
32	0.0	0.0	0.0	6.658169440242058	0.0
33	0.0	0.0	0.0	6.815431164901664	0.0
34	0.0	0.0	0.0	6.9708774583963695	0.0
35	0.0	0.0	0.0	7.143948562783661	0.0
36	0.0	0.0	0.0	7.3089258698941	0.0
37	0.0	0.0	0.0	7.4745839636913765	0.0
38	0.0	0.0	0.0	7.658774583963692	0.0
39	7.5642965204236E-5	0.0	0.0	7.899924357034796	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	7140	0.0	42.195377	1
GCGATAC	125	0.0	41.4	9
TGTAGCG	90	0.0	40.0	1
GGCGATA	605	0.0	38.67769	8
TTTAGCG	105	0.0	38.57143	1
TAGGGTG	1400	0.0	38.410713	5
TATGGGT	1165	0.0	38.04721	4
AGGGTAC	1035	0.0	38.04348	6
GCGATAA	95	0.0	37.894737	9
TATACGG	95	0.0	37.894737	2
ATAGGGT	1200	0.0	37.875	4
TCGTTGA	125	0.0	37.800003	24
CGACGGT	60	1.5643309E-10	37.499996	28
ATTCGCG	30	1.14001465E-4	37.499996	1
ATAACGC	60	1.5643309E-10	37.499996	11
CGATCAA	30	1.14001465E-4	37.499996	10
TATAACG	30	1.14001465E-4	37.499996	1
GTACGCA	30	1.14001465E-4	37.499996	9
ACGGGTA	415	0.0	37.409637	5
GGGCGAT	2900	0.0	37.08621	7
>>END_MODULE