FastQCFastQC Report
Thu 26 May 2016
SRR1547083_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547083_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences678310
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41530.6122569326709026No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC9140.13474664976190828No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG6840.10083884949359438No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG351.2109376E-745.0000041
TACGGGA1400.045.0000044
TGATTCG302.1641808E-645.0000041
GCGATAC351.2109376E-745.0000049
GCGTATG302.1641808E-645.0000041
GCATATT351.2109376E-745.0000049
CAAACGA207.03124E-445.044
TTAATCG207.03124E-445.020
TTTACGG406.8084773E-945.02
GATCCAA207.03124E-445.033
TCTAGCG253.88911E-545.01
ATCGTAG207.03124E-445.01
AAACGAC253.88911E-545.045
GTAGCAT551.8189894E-1245.029
CGCTATT253.88911E-545.040
ACTACGG453.8380676E-1045.02
GCGATAA406.8084773E-945.09
GGTTACG207.03124E-445.01
CGATACT207.03124E-445.010
TACGTAG253.88911E-545.01