##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547083_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	678310
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.680800813787208	31.0	31.0	33.0	30.0	34.0
2	32.06052542347894	33.0	31.0	34.0	30.0	34.0
3	32.12979021391399	33.0	31.0	34.0	30.0	34.0
4	35.80208016983385	37.0	35.0	37.0	35.0	37.0
5	35.74667040143887	37.0	35.0	37.0	33.0	37.0
6	35.80250622871549	37.0	35.0	37.0	35.0	37.0
7	36.12652474532293	37.0	35.0	37.0	35.0	37.0
8	36.072975483186156	37.0	35.0	37.0	35.0	37.0
9	37.8639235747667	39.0	38.0	39.0	35.0	39.0
10	37.40108504960859	39.0	37.0	39.0	35.0	39.0
11	37.08935884772449	39.0	37.0	39.0	33.0	39.0
12	36.25827129188719	38.0	35.0	39.0	33.0	39.0
13	35.86067579720187	38.0	35.0	39.0	31.0	39.0
14	36.88544175966741	39.0	35.0	41.0	32.0	41.0
15	37.14068936032198	39.0	35.0	41.0	32.0	41.0
16	37.30239123704501	39.0	35.0	41.0	33.0	41.0
17	37.2799427990152	39.0	35.0	41.0	33.0	41.0
18	37.21857410328611	39.0	35.0	41.0	33.0	41.0
19	37.15222538367413	38.0	35.0	41.0	32.0	41.0
20	36.96037357550382	38.0	35.0	41.0	32.0	41.0
21	36.86507349147145	38.0	35.0	41.0	32.0	41.0
22	36.77336026300659	38.0	35.0	41.0	32.0	41.0
23	36.75116097359614	38.0	35.0	41.0	32.0	41.0
24	36.663712756704165	38.0	35.0	41.0	32.0	41.0
25	36.578786985301704	38.0	35.0	41.0	32.0	41.0
26	36.42824077486695	38.0	35.0	40.0	31.0	41.0
27	36.33201043770547	38.0	35.0	40.0	31.0	41.0
28	36.376810013120846	38.0	35.0	40.0	31.0	41.0
29	36.392378116200554	37.0	35.0	40.0	31.0	41.0
30	36.3872934204125	37.0	35.0	40.0	31.0	41.0
31	36.22425439695714	37.0	35.0	40.0	31.0	41.0
32	36.082416594182604	38.0	35.0	40.0	31.0	41.0
33	35.95963497515885	38.0	35.0	40.0	30.0	41.0
34	35.822749185475665	37.0	35.0	40.0	30.0	41.0
35	35.71693620910793	37.0	35.0	40.0	30.0	41.0
36	35.59666819006059	37.0	35.0	40.0	30.0	41.0
37	35.508801285547904	37.0	35.0	40.0	29.0	41.0
38	35.47160295440138	37.0	35.0	40.0	29.0	41.0
39	35.428638822957055	37.0	35.0	40.0	29.0	41.0
40	35.29244150904454	37.0	35.0	40.0	29.0	41.0
41	35.29065913815217	36.0	35.0	40.0	29.0	41.0
42	35.21320635107841	36.0	34.0	40.0	29.0	41.0
43	35.087321431204025	36.0	34.0	40.0	28.0	41.0
44	34.93063496041633	36.0	34.0	40.0	27.0	41.0
45	34.84097683949816	36.0	34.0	40.0	27.0	41.0
46	34.79274815349914	35.0	34.0	40.0	27.0	41.0
47	34.74588167651959	35.0	34.0	40.0	27.0	41.0
48	34.68174138668161	35.0	34.0	40.0	27.0	41.0
49	34.6226946381448	36.0	34.0	40.0	27.0	41.0
50	34.49677728472233	35.0	34.0	40.0	26.0	41.0
51	34.14921201220681	35.0	34.0	39.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	2.0
11	0.0
12	1.0
13	2.0
14	6.0
15	8.0
16	34.0
17	101.0
18	244.0
19	415.0
20	818.0
21	1193.0
22	1954.0
23	2992.0
24	4517.0
25	6626.0
26	9294.0
27	10910.0
28	11520.0
29	12967.0
30	15010.0
31	18451.0
32	23163.0
33	31063.0
34	59828.0
35	113587.0
36	43368.0
37	55433.0
38	82866.0
39	171775.0
40	160.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.91209034217393	23.632409960047767	29.303415842313985	14.152083855464316
2	31.784877121080328	25.062139729622153	29.991301912105083	13.161681237192436
3	29.500965635181554	24.199407350621396	32.42249119134319	13.877135822853855
4	26.58813816691483	27.168108976721555	32.016334714216214	14.227418142147396
5	24.426147336763428	31.88453656882546	29.386416240362077	14.302899854049034
6	21.852692721616958	40.21686249649865	26.751043033421297	11.179401748463091
7	86.36021877902435	4.417006973212838	7.248750571272722	1.9740236764901005
8	87.38084356710058	3.421002196635756	6.815173003493978	2.3829812327696773
9	83.16978962421312	5.294187023632262	8.468546829620676	3.0674765225339446
10	52.7788179445976	21.36235644469343	14.4405950081821	11.418230602526869
11	45.4687384824048	20.766905986938124	18.931314590673882	14.833040939983194
12	40.735356990166736	19.955625009214074	23.670150815998582	15.6388671846206
13	22.85739558608896	36.44587283100647	24.107266589022718	16.589464993881855
14	16.45707714761687	38.43537615544515	28.399551827335586	16.707994869602395
15	16.044728811310463	23.492503427636333	44.94331500346449	15.519452757588715
16	17.55642700240303	19.030679187981896	43.47584437793929	19.93704943167578
17	17.859533251758045	20.389202576992822	28.587813831434005	33.16345033981513
18	22.277719626719346	24.28565110347776	31.93584054488361	21.500788724919285
19	28.950627294304965	26.218543143990207	24.466246996211172	20.364582565493652
20	31.9701906208076	23.909274520499476	24.73854137488759	19.381993483805342
21	23.524347274844835	28.40191063083251	27.09085816219722	20.98288393212543
22	23.310138432280226	24.816381890286152	25.457091890138727	26.416387787294894
23	21.25783196473589	29.247836534917663	24.952750217452195	24.54158128289425
24	21.751264171249137	23.509752178207606	35.257625569429905	19.481358081113356
25	19.229408382597928	25.550117203048757	32.51772788253159	22.70274653182173
26	18.22057761200631	34.18245345048724	26.684406834633133	20.912562102873316
27	20.599430938656365	31.210655894797362	27.932803585381315	20.257109581164954
28	17.815010835753565	27.78906399728738	36.42434874909702	17.97157641786204
29	18.01123380165411	24.84439268181215	35.04695493211069	22.09741858442305
30	21.133257655054475	27.89358847724492	29.95341363093571	21.0197402367649
31	28.11811708510858	25.857498783741946	24.761539708982617	21.262844422166857
32	29.583966033229643	26.05003611917855	25.708451887779926	18.657545959811888
33	27.63883769957689	26.7006236086745	24.976190827202903	20.68434786454571
34	20.977429199038788	26.651531010894725	28.231781928616712	24.139257861449778
35	21.32166708437145	25.18641918886645	30.161725464758	23.330188262004096
36	28.701036399286462	24.93166841119842	25.829340566997388	20.53795462251773
37	21.762026212203857	30.173224631805518	27.98425498665802	20.080494169332606
38	21.29129748934853	30.852707464138817	25.102239389069897	22.753755657442763
39	22.097860860078725	28.952691247364776	26.651383585676165	22.298064306880335
40	24.059205967772847	24.803555896271618	26.103846323952173	25.033391812003362
41	17.941206822839113	24.366145272810368	28.188144063923577	29.504503840426942
42	22.45743096814141	25.43822146216332	24.240686411817606	27.86366115787767
43	23.006884757706654	25.792779112795035	25.374238917309196	25.82609721218912
44	20.785039288820744	28.768409724167416	27.54537011101119	22.901180876000648
45	19.042620630685082	33.317509693208116	24.001710132535273	23.638159543571525
46	22.353053913402427	30.454511948813963	25.940056906134362	21.25237723164925
47	22.418658135660685	25.662897495245534	27.467382170394067	24.45106219869971
48	23.328124308944286	22.26194512833365	30.943963674426143	23.46596688829591
49	21.322699060901357	22.897642670755257	30.90799192109802	24.871666347245363
50	19.15097816632513	29.653698161607522	27.50187967153661	23.693444000530732
51	18.87691468502602	31.121316212351285	24.679129011808758	25.322640090813938
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	544.0
1	740.5
2	937.0
3	3803.0
4	6669.0
5	4686.0
6	2703.0
7	2763.5
8	2824.0
9	3013.5
10	3203.0
11	3131.0
12	3059.0
13	3013.5
14	2968.0
15	2835.5
16	2703.0
17	2519.0
18	2335.0
19	2290.0
20	2245.0
21	2268.0
22	2291.0
23	2323.5
24	2356.0
25	2788.5
26	3681.0
27	4141.0
28	5383.0
29	6625.0
30	7894.0
31	9163.0
32	10329.5
33	11496.0
34	12844.0
35	14192.0
36	14997.0
37	15802.0
38	19751.0
39	23700.0
40	32536.5
41	41373.0
42	49240.0
43	57107.0
44	59976.0
45	62845.0
46	60548.5
47	58252.0
48	57278.0
49	56304.0
50	52864.0
51	49424.0
52	44898.0
53	40372.0
54	37334.5
55	34297.0
56	32533.5
57	30770.0
58	30195.0
59	29620.0
60	27878.5
61	26137.0
62	23789.0
63	21441.0
64	18128.0
65	14815.0
66	12898.5
67	10982.0
68	9100.5
69	7219.0
70	6406.0
71	5593.0
72	4779.5
73	3966.0
74	2960.5
75	1618.5
76	1282.0
77	1034.5
78	787.0
79	587.0
80	387.0
81	258.0
82	129.0
83	92.0
84	55.0
85	29.5
86	4.0
87	6.0
88	8.0
89	6.0
90	4.0
91	2.5
92	1.0
93	0.5
94	0.0
95	1.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	678310.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.018640537805616
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.03479857830983	21.083941722274183
2	6.69216823750541	3.616265760553577
3	2.28721001636569	1.8539191579995926
4	1.336298693957188	1.4441989625267353
5	0.8933703334153127	1.2068825952843945
6	0.6775723882167755	1.098425087734292
7	0.5640548906027032	1.0667997432949998
8	0.5149663341126811	1.1130952216369632
9	0.4270659552414539	1.0384867377543083
>10	7.9466880235576065	54.05109647865272
>50	0.5741596518064501	9.475407869066537
>100	0.04944915661490928	2.0169886214260973
>500	0.001648305220496976	0.31797965901926106
>1k	5.494350734989921E-4	0.6165123827763731
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4153	0.6122569326709026	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	914	0.13474664976190828	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	684	0.10083884949359438	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08653860329347939	0.0
2	0.0	0.0	0.0	0.2806976161342159	0.0
3	0.0	0.0	0.0	0.41367516327342957	0.0
4	0.0	0.0	0.0	0.6241983753740915	0.0
5	0.0	0.0	0.0	1.1022983591573174	0.0
6	0.0	0.0	0.0	1.6529315504710236	0.0
7	0.0	0.0	0.0	1.9853754183190577	0.0
8	0.0	0.0	0.0	2.7195530067373324	0.0
9	0.0	0.0	0.0	3.024428358715042	0.0
10	0.0	0.0	0.0	3.564299509074022	0.0
11	0.0	0.0	0.0	4.1085934159897395	0.0
12	0.0	0.0	0.0	4.520646901859032	0.0
13	0.0	0.0	0.0	4.724683404343147	0.0
14	0.0	0.0	0.0	4.8088632041397	0.0
15	0.0	0.0	0.0	4.927982780734473	0.0
16	0.0	0.0	0.0	5.171529241792101	0.0
17	0.0	0.0	0.0	5.467853931093453	0.0
18	0.0	0.0	0.0	5.840102607952116	1.4742521855788652E-4
19	0.0	0.0	0.0	6.043254559124884	1.4742521855788652E-4
20	0.0	0.0	0.0	6.2430157302708205	1.4742521855788652E-4
21	0.0	0.0	0.0	6.504253217555395	1.4742521855788652E-4
22	0.0	0.0	0.0	6.781854904099895	1.4742521855788652E-4
23	0.0	0.0	0.0	7.118279252848993	1.4742521855788652E-4
24	0.0	0.0	0.0	7.35563385472719	1.4742521855788652E-4
25	0.0	0.0	0.0	7.559817782429862	1.4742521855788652E-4
26	0.0	0.0	0.0	7.753092243959252	1.4742521855788652E-4
27	0.0	0.0	0.0	7.938848019342188	1.4742521855788652E-4
28	0.0	0.0	0.0	8.144211348793325	1.4742521855788652E-4
29	0.0	0.0	0.0	8.35385000958264	1.4742521855788652E-4
30	0.0	0.0	0.0	8.60771623593932	1.4742521855788652E-4
31	0.0	0.0	0.0	8.85892880836196	1.4742521855788652E-4
32	0.0	0.0	0.0	9.076823281390515	1.4742521855788652E-4
33	0.0	0.0	0.0	9.291179549173682	1.4742521855788652E-4
34	0.0	0.0	0.0	9.507747195235217	1.4742521855788652E-4
35	0.0	0.0	0.0	9.763382524214592	1.4742521855788652E-4
36	0.0	0.0	0.0	10.011499167047516	1.4742521855788652E-4
37	0.0	0.0	0.0	10.253129100263891	1.4742521855788652E-4
38	0.0	0.0	0.0	10.503899397030857	1.4742521855788652E-4
39	0.0	0.0	0.0	10.74567675546579	1.4742521855788652E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	35	1.2109376E-7	45.000004	1
TACGGGA	140	0.0	45.000004	4
TGATTCG	30	2.1641808E-6	45.000004	1
GCGATAC	35	1.2109376E-7	45.000004	9
GCGTATG	30	2.1641808E-6	45.000004	1
GCATATT	35	1.2109376E-7	45.000004	9
CAAACGA	20	7.03124E-4	45.0	44
TTAATCG	20	7.03124E-4	45.0	20
TTTACGG	40	6.8084773E-9	45.0	2
GATCCAA	20	7.03124E-4	45.0	33
TCTAGCG	25	3.88911E-5	45.0	1
ATCGTAG	20	7.03124E-4	45.0	1
AAACGAC	25	3.88911E-5	45.0	45
GTAGCAT	55	1.8189894E-12	45.0	29
CGCTATT	25	3.88911E-5	45.0	40
ACTACGG	45	3.8380676E-10	45.0	2
GCGATAA	40	6.8084773E-9	45.0	9
GGTTACG	20	7.03124E-4	45.0	1
CGATACT	20	7.03124E-4	45.0	10
TACGTAG	25	3.88911E-5	45.0	1
>>END_MODULE