FastQCFastQC Report
Thu 26 May 2016
SRR1547082_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547082_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences961806
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179721.8685680896147454No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGATC207.0327113E-445.00000430
TATAGCG253.8903298E-545.01
CGTTTTT110800.043.7409741
AACGAGC1400.040.1785715
ACGGGCT2750.040.0909085
TCTAGCG403.457444E-739.3750041
AATACGG1200.039.3750042
GCGATAA750.039.09
ACGGGAT3850.038.571435
ATAACGG1050.038.571432
GTTTTTT127900.038.5613752
GACCGAT2750.038.4545449
TCATCGA659.094947E-1238.0769216
TTCATCG659.094947E-1238.0769215
ACGACCA1300.038.0769228
TAGGGAT10650.037.8169025
CGATACG301.1397833E-437.50000410
TCGATCA3300.037.517
GGGCGAT10950.037.191787
CCTCGAT3450.037.17391215