FastQCFastQC Report
Thu 26 May 2016
SRR1547081_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547081_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences829489
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58490.705132919182774No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACCG253.889863E-545.01
CGTTTTT33950.042.9455071
TGCGTAG603.6379788E-1241.2499961
ATAACGC501.0804797E-940.511
AACGTAG451.9272193E-840.01
GTATGCG403.456662E-739.3751
TCGTAAG403.456662E-739.3751
AATACGG800.039.3752
ACGTAGG1200.039.3749962
TTGCGAG1150.039.1304321
CGACGGG1500.039.0000043
TACGCGG750.039.0000042
TAGGGAC5250.039.05
TACGGGA2200.038.8636364
TTACGGG2900.038.017243
CAATAGG950.037.8947372
ACCCGCT2500.037.834
ACGGGAT2100.037.55
CTATACG301.13964816E-437.4999961
GCGATCG301.13964816E-437.4999969
Produced by FastQC (version 0.11.5)