##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547081_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	829489
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.634581049296614	31.0	31.0	33.0	30.0	34.0
2	32.02916856040285	33.0	31.0	34.0	30.0	34.0
3	32.10627868482885	33.0	31.0	34.0	30.0	34.0
4	35.78886519290792	37.0	35.0	37.0	35.0	37.0
5	35.737051365358674	37.0	35.0	37.0	33.0	37.0
6	35.79289417942854	37.0	35.0	37.0	35.0	37.0
7	36.09614111820651	37.0	35.0	37.0	35.0	37.0
8	36.046574457286354	37.0	35.0	37.0	35.0	37.0
9	37.8264835338383	39.0	38.0	39.0	35.0	39.0
10	37.385923140632364	39.0	37.0	39.0	35.0	39.0
11	37.09216758751472	39.0	37.0	39.0	33.0	39.0
12	36.40337725997572	38.0	35.0	39.0	33.0	39.0
13	36.1368481076904	39.0	35.0	39.0	32.0	39.0
14	37.16249522296257	39.0	35.0	41.0	32.0	41.0
15	37.368321942786466	39.0	35.0	41.0	32.0	41.0
16	37.49972694032109	39.0	35.0	41.0	33.0	41.0
17	37.46798812280814	39.0	35.0	41.0	33.0	41.0
18	37.35391066065976	39.0	35.0	41.0	32.0	41.0
19	37.28775306242759	39.0	35.0	41.0	32.0	41.0
20	37.10655234728851	39.0	35.0	41.0	32.0	41.0
21	37.03148444403723	39.0	35.0	41.0	32.0	41.0
22	36.95617904517118	38.0	35.0	41.0	32.0	41.0
23	36.92442937760477	38.0	35.0	41.0	32.0	41.0
24	36.845883429436675	38.0	35.0	41.0	32.0	41.0
25	36.73941547145291	38.0	35.0	41.0	32.0	41.0
26	36.60911356268739	38.0	35.0	41.0	31.0	41.0
27	36.51005257453685	38.0	35.0	41.0	31.0	41.0
28	36.52438790628929	38.0	35.0	41.0	31.0	41.0
29	36.53328133344746	38.0	35.0	41.0	31.0	41.0
30	36.48593772792647	38.0	35.0	41.0	31.0	41.0
31	36.34392861147044	38.0	35.0	41.0	31.0	41.0
32	36.202933372232785	38.0	35.0	41.0	30.0	41.0
33	36.055473912252	38.0	35.0	41.0	30.0	41.0
34	35.88809737079093	38.0	35.0	41.0	30.0	41.0
35	35.762328373251485	38.0	35.0	41.0	29.0	41.0
36	35.66860681696804	38.0	35.0	41.0	29.0	41.0
37	35.59189573339731	38.0	35.0	41.0	29.0	41.0
38	35.522015361264586	38.0	35.0	40.0	29.0	41.0
39	35.432759204763414	38.0	35.0	40.0	28.0	41.0
40	35.295414405736544	37.0	35.0	40.0	27.0	41.0
41	35.249014754867154	37.0	34.0	40.0	27.0	41.0
42	35.15694843451812	37.0	34.0	40.0	27.0	41.0
43	35.035055317189254	37.0	34.0	40.0	27.0	41.0
44	34.89551398511614	37.0	34.0	40.0	26.0	41.0
45	34.80409384572912	36.0	34.0	40.0	26.0	41.0
46	34.76376419699357	36.0	34.0	40.0	26.0	41.0
47	34.70882555404592	36.0	34.0	40.0	26.0	41.0
48	34.632018025555496	36.0	34.0	40.0	26.0	41.0
49	34.555515504123626	36.0	34.0	40.0	26.0	41.0
50	34.43488340411989	35.0	34.0	40.0	26.0	41.0
51	34.087092173615325	35.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	6.0
13	5.0
14	5.0
15	33.0
16	71.0
17	132.0
18	341.0
19	645.0
20	1142.0
21	1849.0
22	2818.0
23	4006.0
24	5801.0
25	8738.0
26	11646.0
27	13886.0
28	15072.0
29	16328.0
30	18533.0
31	22119.0
32	27695.0
33	36694.0
34	67684.0
35	111533.0
36	55931.0
37	75120.0
38	112767.0
39	218715.0
40	172.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.385962924161745	23.552211060062277	29.121181836046045	14.940644179729931
2	33.006224314005365	24.144262310892607	29.356989664721294	13.492523710380727
3	29.603044766115044	24.10327322001859	32.23406217562861	14.059619838237758
4	27.05267942070359	26.460869282172517	31.685531694814518	14.800919602309373
5	24.26313067442727	30.98365379167174	30.315049385826697	14.438166148074297
6	22.26768528576027	39.630423067695894	27.073053409990973	11.028838236552865
7	86.7604030915419	3.7564090663046765	7.630601490797346	1.852586351356076
8	87.75547355058355	2.862123548353263	7.305099886797775	2.077303014265409
9	83.54408557557726	4.31132902304913	9.046533468195479	3.0980519331781373
10	49.31710969042386	24.210447637039188	14.399588180192865	12.072854492344081
11	40.814163900907666	21.60498813124707	22.193061029139628	15.387786938705636
12	35.77551962714394	20.074648367850568	26.937789410106706	17.212042594898787
13	24.696891700794104	28.8522210662227	27.341049730617286	19.109837502365913
14	18.89030475389065	31.476125662908128	30.6157164230026	19.017853160198626
15	17.971184669115566	22.503131445986625	41.41935577204761	18.1063281128502
16	22.031636344785767	20.37061371519092	37.681994577384394	19.915755362638926
17	22.45418564923706	20.492495982466313	28.28669216831085	28.766626199985772
18	24.654817604573417	22.38558920009789	31.733995266965564	21.225597928363126
19	28.431359547866215	23.77053824704125	26.54513803076352	21.252964174329016
20	29.920227995790178	23.963307530298774	26.188291827860283	19.92817264605076
21	26.470393217993248	25.173329604129773	28.5159899649061	19.84028721297088
22	24.307254225191656	22.21753392751441	29.091525023237196	24.38368682405674
23	23.03309627975778	25.083756384955073	27.837499954791443	24.0456473804957
24	23.032131830560743	22.557863937918405	33.56705152208166	20.842952709439185
25	21.841880965268977	23.75920596897608	31.45948891425926	22.93942415149568
26	20.838371575753264	28.37469815754037	27.497290500537076	23.289639766169294
27	21.335304024525943	27.021696490248814	29.816188038659945	21.826811446565294
28	19.624612261283755	25.66122034168024	33.79237096573915	20.92179643129686
29	20.69671809993864	23.769935466293106	33.19465357587623	22.338692857892028
30	22.673838953861956	24.821305647211716	30.510712016675328	21.994143382251
31	27.28028943120403	23.783076086602716	26.864973495730503	22.07166098646275
32	28.244618072090166	24.20164703811624	26.567199806145712	20.986535083647883
33	27.274623292171444	24.761389240845872	26.952858928810386	21.011128538172297
34	22.589570205270956	25.22999099445562	29.11804737615568	23.062391424117738
35	22.490231937976272	24.773806524257704	29.939637535880525	22.7963240018855
36	27.789398051089286	23.963186974149146	26.773712490460994	21.473702484300574
37	23.478310140339413	27.7109159976805	27.449188596834915	21.361585265145166
38	23.373185177862517	28.121891911767367	24.991772042787787	23.51315086758233
39	23.288313648523367	26.92488990209635	27.042311591835457	22.744484857544826
40	24.415151979110032	24.02768451420091	27.412539527347562	24.1446239793415
41	21.13144357550251	23.562699445080042	27.366004853590585	27.939852125826864
42	23.510619188440113	25.21528314420083	25.776713133025268	25.49738453433379
43	23.375957969303993	24.598758994995716	26.878958009087523	25.14632502661277
44	22.264912494318793	26.04965225578639	28.162037109593978	23.523398140300834
45	21.264417008543816	28.580608061107498	25.969241303983537	24.185733626365145
46	23.81707292079823	26.778655292595804	27.06799005170653	22.336281734899437
47	22.784991723820326	24.745596385244408	28.256312018604223	24.213099872331036
48	23.220199423982717	23.490847979900877	29.388575375924216	23.90037722019219
49	23.367277926530672	22.246226291126224	30.13976074426545	24.24673503807766
50	21.735188772846897	26.563583121656826	28.044735976004503	23.65649212949177
51	20.68888195021272	28.267282628220507	26.263398309079445	24.780437112487327
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	420.0
1	799.5
2	1179.0
3	5576.0
4	9973.0
5	6950.5
6	3928.0
7	3792.5
8	3657.0
9	3815.0
10	3973.0
11	3914.0
12	3855.0
13	3688.5
14	3522.0
15	3413.5
16	3305.0
17	3145.0
18	2985.0
19	2922.0
20	2859.0
21	2840.0
22	2821.0
23	3402.5
24	3984.0
25	4257.0
26	4979.0
27	5428.0
28	6253.5
29	7079.0
30	8632.5
31	10186.0
32	11534.5
33	12883.0
34	14118.5
35	15354.0
36	17204.0
37	19054.0
38	22697.5
39	26341.0
40	32447.5
41	38554.0
42	47326.5
43	56099.0
44	58665.0
45	61231.0
46	62185.5
47	63140.0
48	62921.5
49	62703.0
50	60457.5
51	58212.0
52	55916.0
53	53620.0
54	50027.5
55	46435.0
56	45988.5
57	45542.0
58	44208.0
59	42874.0
60	41547.5
61	40221.0
62	36914.5
63	33608.0
64	29840.5
65	26073.0
66	22271.5
67	18470.0
68	15367.5
69	12265.0
70	10879.5
71	9494.0
72	7782.0
73	6070.0
74	4996.0
75	2826.0
76	1730.0
77	1390.0
78	1050.0
79	802.5
80	555.0
81	367.0
82	179.0
83	126.0
84	73.0
85	45.0
86	17.0
87	14.0
88	11.0
89	15.5
90	20.0
91	11.5
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	829489.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.297521860295614
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.2972234462723	20.064279014582052
2	5.579833602588799	2.9347159228178197
3	2.123029998716305	1.674912834039162
4	1.344041145262591	1.4137980559471897
5	1.011188251681027	1.3295872576727956
6	0.838757201311382	1.3234341502179863
7	0.734922242714077	1.3528643620373646
8	0.68352137037195	1.4379934543468456
9	0.6192916815667848	1.465725288051158
>10	10.53750626103159	61.868739016391814
>50	0.2044392226117573	3.298398913814888
>100	0.02578512717194961	1.1273162218519337
>500	0.0	0.0
>1k	0.0	0.0
>5k	4.604486994991002E-4	0.7082355082289968
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5849	0.705132919182774	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04303854541772103	0.0
2	0.0	0.0	0.0	0.15419131537609299	0.0
3	0.0	0.0	0.0	0.23713394632116883	0.0
4	0.0	0.0	0.0	0.36600847027507294	0.0
5	0.0	0.0	0.0	0.674149988728	0.0
6	0.0	0.0	0.0	1.0331662023245636	0.0
7	0.0	0.0	0.0	1.2795829721672016	0.0
8	0.0	0.0	0.0	1.8101505866865022	0.0
9	0.0	0.0	0.0	2.0711546506343064	0.0
10	0.0	0.0	0.0	2.4476515059271433	0.0
11	0.0	0.0	0.0	2.8012426927903804	0.0
12	0.0	0.0	0.0	3.1114336657870085	0.0
13	0.0	0.0	0.0	3.2524843608534892	0.0
14	0.0	0.0	0.0	3.3202369169452517	0.0
15	0.0	0.0	0.0	3.3966695158103364	0.0
16	0.0	0.0	0.0	3.545676916752362	0.0
17	0.0	0.0	0.0	3.7215683390617595	0.0
18	0.0	0.0	0.0	3.9473700073177582	0.0
19	0.0	0.0	0.0	4.075762306673145	0.0
20	0.0	0.0	0.0	4.217415782487772	0.0
21	0.0	0.0	0.0	4.378840466841634	0.0
22	0.0	0.0	0.0	4.554611333001402	0.0
23	0.0	0.0	0.0	4.747742284707814	0.0
24	0.0	0.0	0.0	4.8934946696098445	0.0
25	0.0	0.0	0.0	5.021284188217083	0.0
26	0.0	0.0	0.0	5.150038156021358	0.0
27	0.0	0.0	0.0	5.276501556982672	0.0
28	0.0	0.0	0.0	5.410680551520273	0.0
29	0.0	0.0	0.0	5.558000166367487	0.0
30	0.0	0.0	0.0	5.759570048547961	0.0
31	0.0	0.0	0.0	5.917378048412939	0.0
32	0.0	0.0	0.0	6.068073235449777	0.0
33	0.0	0.0	0.0	6.221058989329575	0.0
34	0.0	0.0	0.0	6.39068149185824	0.0
35	0.0	0.0	0.0	6.5715157163024465	0.0
36	0.0	0.0	0.0	6.740294325783705	0.0
37	0.0	0.0	0.0	6.91220739515533	0.0
38	0.0	0.0	0.0	7.120648977864685	0.0
39	0.0	0.0	0.0	7.399615908107281	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAACCG	25	3.889863E-5	45.0	1
CGTTTTT	3395	0.0	42.945507	1
TGCGTAG	60	3.6379788E-12	41.249996	1
ATAACGC	50	1.0804797E-9	40.5	11
AACGTAG	45	1.9272193E-8	40.0	1
GTATGCG	40	3.456662E-7	39.375	1
TCGTAAG	40	3.456662E-7	39.375	1
AATACGG	80	0.0	39.375	2
ACGTAGG	120	0.0	39.374996	2
TTGCGAG	115	0.0	39.130432	1
CGACGGG	150	0.0	39.000004	3
TACGCGG	75	0.0	39.000004	2
TAGGGAC	525	0.0	39.0	5
TACGGGA	220	0.0	38.863636	4
TTACGGG	290	0.0	38.01724	3
CAATAGG	95	0.0	37.894737	2
ACCCGCT	250	0.0	37.8	34
ACGGGAT	210	0.0	37.5	5
CTATACG	30	1.13964816E-4	37.499996	1
GCGATCG	30	1.13964816E-4	37.499996	9
>>END_MODULE