FastQCFastQC Report
Thu 26 May 2016
SRR1547079_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547079_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences710035
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23660.3332230101333033No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC15960.2247776518058969No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCATA253.889293E-545.00000437
GCAAACG253.889293E-545.0000041
CGTCTTA253.889293E-545.00000423
TCGATAG351.2110468E-745.0000041
CGCTCGA253.889293E-545.00000437
TACGACC253.889293E-545.00000411
CGGGCAA253.889293E-545.0000046
GTATCGG351.2110468E-745.0000042
GCTACGA351.2110468E-745.00000410
TATCGTC351.2110468E-745.00000433
TATCGGA253.889293E-545.00000420
GCCCGTT253.889293E-545.00000421
GTACGAC253.889293E-545.00000410
AGCCGAC207.0314633E-445.027
CTCACGT406.8084773E-945.045
TCGCAAG207.0314633E-445.01
CGACGTG207.0314633E-445.021
CGACGGT453.8380676E-1045.028
CTTCACG207.0314633E-445.018
GCTATCC207.0314633E-445.023