##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547079_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	710035
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.675896258635138	31.0	31.0	33.0	30.0	34.0
2	32.06173357651383	33.0	31.0	34.0	30.0	34.0
3	32.15413606371517	33.0	31.0	34.0	30.0	34.0
4	35.797240981078396	37.0	35.0	37.0	35.0	37.0
5	35.73672142922532	37.0	35.0	37.0	33.0	37.0
6	35.798309942467625	37.0	35.0	37.0	35.0	37.0
7	36.140752216440035	37.0	35.0	37.0	35.0	37.0
8	36.09025329737266	37.0	35.0	37.0	35.0	37.0
9	37.892878520072955	39.0	38.0	39.0	35.0	39.0
10	37.453194560831506	39.0	37.0	39.0	35.0	39.0
11	37.06913462012436	39.0	37.0	39.0	33.0	39.0
12	35.87371045089326	37.0	35.0	39.0	32.0	39.0
13	35.28014252818523	37.0	35.0	39.0	30.0	39.0
14	36.178130655531064	38.0	35.0	41.0	31.0	41.0
15	36.567180491102555	38.0	35.0	41.0	32.0	41.0
16	36.81302330166823	38.0	35.0	41.0	32.0	41.0
17	36.811955748660274	38.0	35.0	41.0	32.0	41.0
18	36.79207926369827	37.0	35.0	41.0	32.0	41.0
19	36.751922088347754	37.0	35.0	41.0	32.0	41.0
20	36.55689367425549	36.0	35.0	40.0	32.0	41.0
21	36.426204342039476	36.0	35.0	40.0	32.0	41.0
22	36.326805016654106	36.0	35.0	40.0	32.0	41.0
23	36.35320512369108	36.0	35.0	40.0	32.0	41.0
24	36.2407881301626	36.0	35.0	40.0	32.0	41.0
25	36.16033857485898	36.0	35.0	40.0	31.0	41.0
26	36.02151865753097	35.0	35.0	40.0	31.0	41.0
27	35.95678804565972	35.0	35.0	40.0	31.0	41.0
28	36.03045765349596	36.0	35.0	40.0	31.0	41.0
29	36.09666143218292	36.0	35.0	40.0	31.0	41.0
30	36.0727907779194	36.0	35.0	40.0	31.0	41.0
31	35.91413944383024	36.0	35.0	40.0	31.0	41.0
32	35.74659840712078	35.0	35.0	40.0	31.0	41.0
33	35.658846394896024	36.0	35.0	40.0	30.0	41.0
34	35.578289802615366	36.0	35.0	40.0	30.0	41.0
35	35.53000063377157	36.0	35.0	40.0	30.0	41.0
36	35.3832796974797	36.0	35.0	40.0	30.0	41.0
37	35.29976409613611	36.0	34.0	40.0	29.0	41.0
38	35.23725309315738	35.0	34.0	40.0	29.0	41.0
39	35.23497996577633	36.0	34.0	40.0	29.0	41.0
40	35.101597808558736	36.0	34.0	40.0	29.0	41.0
41	35.1179716492849	35.0	34.0	40.0	29.0	41.0
42	35.03111114240847	35.0	34.0	40.0	29.0	41.0
43	34.905873654115645	35.0	34.0	40.0	29.0	41.0
44	34.772647827219785	35.0	34.0	40.0	28.0	41.0
45	34.6796242438753	35.0	34.0	40.0	27.0	41.0
46	34.657736590449765	35.0	34.0	40.0	28.0	41.0
47	34.62866619251164	35.0	34.0	40.0	28.0	41.0
48	34.61613582429035	35.0	34.0	40.0	28.0	41.0
49	34.6151175646271	35.0	34.0	40.0	28.0	41.0
50	34.4757272528819	35.0	34.0	39.0	27.0	41.0
51	34.10566662206793	35.0	34.0	39.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	1.0
13	1.0
14	8.0
15	19.0
16	52.0
17	105.0
18	278.0
19	524.0
20	878.0
21	1415.0
22	2191.0
23	3247.0
24	4634.0
25	6830.0
26	9389.0
27	11250.0
28	12200.0
29	13785.0
30	16477.0
31	20144.0
32	25785.0
33	34887.0
34	72017.0
35	148859.0
36	38188.0
37	49316.0
38	75351.0
39	162022.0
40	180.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.63332089263205	23.769250811579713	30.186258423880513	13.411169871907722
2	30.30639334680685	25.83534614490835	30.179639031878708	13.678621476406091
3	29.68473385114818	24.388516059067513	32.394741104311755	13.532008985472546
4	25.67479067933271	27.454421260923755	31.519432140669124	15.351355919074411
5	23.722774229439395	32.49670790876506	29.7381115015457	14.042406360249846
6	22.635081369228278	40.93812276859591	25.17326610660038	11.25352975557543
7	88.6215468251565	3.6989725858584435	5.967452308689008	1.7120282802960418
8	89.48699711986029	2.7221193321455983	5.449590513143718	2.341293034850395
9	86.01759068214947	4.109234051842515	7.192603181533305	2.680572084474709
10	58.357968269169824	21.822163696155826	10.686797129718958	9.13307090495539
11	53.5282063560247	16.96296661432183	17.99601428098615	11.51281274866732
12	47.04035716549184	19.711141000091544	19.884512735287696	13.363989099128917
13	22.360165343961917	42.426781778363036	21.474997711380425	13.738055166294618
14	14.180850239776913	44.53287513995789	26.557845739998733	14.728428880266467
15	13.615948509580514	21.856246523058722	51.25127634553226	13.2765286218285
16	15.456421162337067	17.249853880442515	48.73280894603787	18.560916011182545
17	15.519657481673438	17.3119634947573	27.9484814128881	39.219897610681166
18	22.20073658340786	22.14904899054272	33.576091319442	22.07412310660742
19	30.118796960713208	24.181343173223855	24.463864457385903	21.235995408677034
20	32.44741456407078	23.433774391403244	22.908307336962263	21.21050370756371
21	22.04004027970452	28.581689635017995	26.827973268923362	22.550296816354123
22	21.762307491884204	25.22284112755005	25.32537128451414	27.689480096051604
23	20.22125669861345	30.699895075594863	23.68756469751491	25.391283528276777
24	20.627997211405074	23.232798383178295	37.29562627194434	18.843578133472295
25	16.32243480955164	24.76751146070264	36.21765124254438	22.692402487201335
26	16.225679015823165	34.965881963565174	27.072468258606968	21.73597076200469
27	18.46275183617709	32.462484243734465	29.02547057539417	20.049293344694274
28	14.828423950931995	28.191990535677817	39.497489560373786	17.4820959530164
29	15.572190103304766	23.87840036054561	38.6864027829614	21.863006753188223
30	19.526643052807255	27.68624081911455	32.02137922778455	20.765736900293646
31	30.57905596203004	24.969332497693774	22.640151541825404	21.81145999845078
32	32.54008605209602	25.800981641750052	23.940791651115788	17.71814065503813
33	27.639764236974234	26.817973761856813	24.08839000894322	21.453871992225736
34	19.14060574478723	26.93219348341983	29.247853979029202	24.679346792763738
35	19.631004105431423	24.46062518044885	31.48211003682917	24.426260677290557
36	30.69862753244558	22.8173259064694	26.58798509932609	19.896061461758926
37	21.485842247213167	29.404184300773906	29.010119219475094	20.099854232537833
38	19.956058504158243	33.11456477497588	23.142802819579316	23.786573901286555
39	20.705880696022028	29.930637222108768	27.551177054652236	21.81230502721697
40	23.770518354728992	24.831311132549803	24.218946953319204	27.179223559402
41	16.549747547656104	22.960699120465893	27.0909180533354	33.398635278542606
42	23.004781454435346	25.45649158140092	23.22829156309196	28.310435401071775
43	22.433682846620236	26.337574908279173	24.905955340229706	26.32278690487089
44	20.02267493855937	31.020583492363052	26.60967417099157	22.34706739808601
45	17.949960213228923	35.38867802291436	22.05637750251748	24.60498426133923
46	22.509312921194024	31.08043969663467	25.769997253656513	20.640250128514793
47	22.233411029033782	24.67300907701733	28.64647517375904	24.44710472018985
48	23.786855577541953	20.66616434401121	30.627645116085827	24.919334962361013
49	20.43349975705423	20.731513235263048	32.74641390917349	26.088573098509226
50	18.564577802502694	30.78003197025499	27.54807861584288	23.10731161139944
51	17.727435971466196	33.049356721851744	23.843049990493427	25.38015731618864
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	340.0
1	561.0
2	782.0
3	2467.0
4	4152.0
5	3090.0
6	2028.0
7	2171.5
8	2315.0
9	2492.0
10	2669.0
11	2684.0
12	2699.0
13	2668.0
14	2637.0
15	2522.5
16	2408.0
17	2241.0
18	2074.0
19	1924.5
20	1775.0
21	1845.5
22	1916.0
23	2045.5
24	2175.0
25	2495.5
26	3388.0
27	3960.0
28	4529.0
29	5098.0
30	5742.5
31	6387.0
32	8356.5
33	10326.0
34	11195.5
35	12065.0
36	13543.5
37	15022.0
38	19423.0
39	23824.0
40	35438.0
41	47052.0
42	59587.0
43	72122.0
44	74176.0
45	76230.0
46	71060.0
47	65890.0
48	64251.5
49	62613.0
50	59983.0
51	57353.0
52	53386.0
53	49419.0
54	43466.0
55	37513.0
56	33875.0
57	30237.0
58	28777.5
59	27318.0
60	25494.5
61	23671.0
62	21603.0
63	19535.0
64	15766.5
65	11998.0
66	9959.0
67	7920.0
68	6573.0
69	5226.0
70	4431.5
71	3637.0
72	3013.5
73	2390.0
74	1717.0
75	923.5
76	803.0
77	564.0
78	325.0
79	208.0
80	91.0
81	108.5
82	126.0
83	74.0
84	22.0
85	17.5
86	13.0
87	9.0
88	5.0
89	5.0
90	5.0
91	4.5
92	4.0
93	2.5
94	1.0
95	1.5
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	710035.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.29343673198102
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.79188425388216	16.990434390855928
2	6.63809427617754	2.8269568098142366
3	2.0309566826935783	1.2973814288498933
4	1.0016236550220823	0.8531203970987318
5	0.6559611321677877	0.6983833433226715
6	0.41980085964353225	0.536340182691048
7	0.38204793632286055	0.5694579492472321
8	0.28240858096416444	0.48107594010631805
9	0.24966714967342546	0.47846444900626095
>10	5.867222175473952	35.6035020761595
>50	2.5425560462576273	35.03621732269273
>100	0.13644607054523916	4.067142897343191
>500	0.0	0.0
>1k	0.0013311811760511136	0.5615228128122705
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2366	0.3332230101333033	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1596	0.2247776518058969	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.408381276979304E-4	0.0	0.0	0.06844733006119416	0.0
2	1.408381276979304E-4	0.0	0.0	0.2540719823670664	0.0
3	1.408381276979304E-4	0.0	0.0	0.35857387311893074	0.0
4	1.408381276979304E-4	0.0	0.0	0.5965903089284331	0.0
5	1.408381276979304E-4	0.0	0.0	1.0181188251283388	0.0
6	1.408381276979304E-4	0.0	0.0	1.4942925348750413	0.0
7	1.408381276979304E-4	0.0	0.0	1.7938552324885393	0.0
8	1.408381276979304E-4	0.0	0.0	2.6252227002894224	0.0
9	1.408381276979304E-4	0.0	0.0	2.99013428915476	0.0
10	1.408381276979304E-4	0.0	0.0	3.540107177815178	0.0
11	1.408381276979304E-4	0.0	0.0	3.9881132620222948	0.0
12	1.408381276979304E-4	0.0	0.0	4.369925426211384	0.0
13	1.408381276979304E-4	0.0	0.0	4.528509157999253	0.0
14	1.408381276979304E-4	0.0	0.0	4.585689437844613	0.0
15	1.408381276979304E-4	0.0	0.0	4.663291246206173	0.0
16	1.408381276979304E-4	0.0	0.0	4.855957804896942	0.0
17	1.408381276979304E-4	0.0	0.0	5.070876787763983	0.0
18	1.408381276979304E-4	0.0	0.0	5.3943819670861295	0.0
19	1.408381276979304E-4	0.0	0.0	5.541416972402769	0.0
20	1.408381276979304E-4	0.0	0.0	5.708310153724816	0.0
21	1.408381276979304E-4	0.0	0.0	5.908722809438971	0.0
22	1.408381276979304E-4	0.0	0.0	6.091389861063187	0.0
23	1.408381276979304E-4	0.0	0.0	6.309829797122677	0.0
24	1.408381276979304E-4	0.0	0.0	6.460526593759463	0.0
25	2.816762553958608E-4	0.0	0.0	6.588830128092277	0.0
26	2.816762553958608E-4	0.0	0.0	6.715302766765019	0.0
27	2.816762553958608E-4	0.0	0.0	6.826001535135592	0.0
28	2.816762553958608E-4	0.0	0.0	6.951629145042146	0.0
29	2.816762553958608E-4	0.0	0.0	7.082608603801221	0.0
30	2.816762553958608E-4	0.0	0.0	7.2842888026646575	0.0
31	2.816762553958608E-4	0.0	0.0	7.4561113184561325	0.0
32	2.816762553958608E-4	0.0	0.0	7.607089791348314	0.0
33	2.816762553958608E-4	0.0	0.0	7.755251501686536	0.0
34	2.816762553958608E-4	0.0	0.0	7.9236939024132615	0.0
35	2.816762553958608E-4	0.0	0.0	8.138472047152606	0.0
36	2.816762553958608E-4	0.0	0.0	8.30423852345307	0.0
37	2.816762553958608E-4	0.0	0.0	8.472399247924399	0.0
38	2.816762553958608E-4	0.0	0.0	8.646897688142134	0.0
39	2.816762553958608E-4	0.0	0.0	8.808720696867056	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCATA	25	3.889293E-5	45.000004	37
GCAAACG	25	3.889293E-5	45.000004	1
CGTCTTA	25	3.889293E-5	45.000004	23
TCGATAG	35	1.2110468E-7	45.000004	1
CGCTCGA	25	3.889293E-5	45.000004	37
TACGACC	25	3.889293E-5	45.000004	11
CGGGCAA	25	3.889293E-5	45.000004	6
GTATCGG	35	1.2110468E-7	45.000004	2
GCTACGA	35	1.2110468E-7	45.000004	10
TATCGTC	35	1.2110468E-7	45.000004	33
TATCGGA	25	3.889293E-5	45.000004	20
GCCCGTT	25	3.889293E-5	45.000004	21
GTACGAC	25	3.889293E-5	45.000004	10
AGCCGAC	20	7.0314633E-4	45.0	27
CTCACGT	40	6.8084773E-9	45.0	45
TCGCAAG	20	7.0314633E-4	45.0	1
CGACGTG	20	7.0314633E-4	45.0	21
CGACGGT	45	3.8380676E-10	45.0	28
CTTCACG	20	7.0314633E-4	45.0	18
GCTATCC	20	7.0314633E-4	45.0	23
>>END_MODULE