FastQCFastQC Report
Thu 26 May 2016
SRR1547078_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547078_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences461834
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20570.445398130064049No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTC207.0288975E-445.0000045
CGTTAGA207.0288975E-445.00000442
GATATAC207.0288975E-445.0000049
GTCGTAA207.0288975E-445.00000422
AACTCGT207.0288975E-445.00000419
AAGTGCG207.0288975E-445.0000041
ATACCCG207.0288975E-445.00000425
ACTACGG253.8871694E-545.02
ATCGGGT351.2098099E-745.04
AATACGG253.8871694E-545.02
TACGTAG351.2098099E-745.01
TAGTACG253.8871694E-545.01
CGGGTAT302.162671E-644.9999966
GCGATAT302.162671E-644.9999969
GGCGATA1000.042.758
CGTTTTT14300.042.6398621
CGCAGTA1200.041.24999637
ACGGGAT1750.041.1428575
TAGGCGG556.002665E-1140.9090922
AGTACGG1050.040.7142872
Produced by FastQC (version 0.11.5)