##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547078_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	461834
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.66735017343894	31.0	31.0	33.0	30.0	34.0
2	32.048679828683035	33.0	31.0	34.0	30.0	34.0
3	32.1421246595097	33.0	31.0	34.0	30.0	34.0
4	35.79907066175292	37.0	35.0	37.0	35.0	37.0
5	35.73970517545265	37.0	35.0	37.0	33.0	37.0
6	35.80153908114171	37.0	35.0	37.0	35.0	37.0
7	36.10796953017751	37.0	35.0	37.0	35.0	37.0
8	36.067229783861734	37.0	35.0	37.0	35.0	37.0
9	37.850916130038065	39.0	38.0	39.0	35.0	39.0
10	37.424286648449446	39.0	37.0	39.0	35.0	39.0
11	37.09557763178978	39.0	37.0	39.0	33.0	39.0
12	36.26770441327403	38.0	35.0	39.0	33.0	39.0
13	35.897898379071265	38.0	35.0	39.0	31.0	39.0
14	36.87542926679283	39.0	35.0	41.0	31.0	41.0
15	37.158015650645034	39.0	35.0	41.0	32.0	41.0
16	37.341269806900314	39.0	35.0	41.0	33.0	41.0
17	37.32176496316858	39.0	35.0	41.0	33.0	41.0
18	37.261864652667406	39.0	35.0	41.0	33.0	41.0
19	37.20675827245287	38.0	35.0	41.0	33.0	41.0
20	37.040070674744605	38.0	35.0	41.0	32.0	41.0
21	36.94163487313624	38.0	35.0	41.0	32.0	41.0
22	36.87281577363295	38.0	35.0	41.0	32.0	41.0
23	36.83798940744943	38.0	35.0	41.0	32.0	41.0
24	36.76033596487049	38.0	35.0	41.0	32.0	41.0
25	36.66031084762057	38.0	35.0	41.0	32.0	41.0
26	36.52804037814453	38.0	35.0	41.0	31.0	41.0
27	36.41860278801474	38.0	35.0	41.0	31.0	41.0
28	36.483377144168685	38.0	35.0	41.0	31.0	41.0
29	36.51594512313948	38.0	35.0	41.0	31.0	41.0
30	36.479735142930146	38.0	35.0	41.0	31.0	41.0
31	36.33925393106614	38.0	35.0	40.0	31.0	41.0
32	36.231483606663865	38.0	35.0	41.0	31.0	41.0
33	36.12549530783788	38.0	35.0	41.0	30.0	41.0
34	36.01704292018344	38.0	35.0	41.0	30.0	41.0
35	35.93887197564493	38.0	35.0	41.0	30.0	41.0
36	35.80288805068488	38.0	35.0	41.0	30.0	41.0
37	35.72565467245807	38.0	35.0	41.0	30.0	41.0
38	35.65797017976156	37.0	35.0	40.0	30.0	41.0
39	35.61769163812106	37.0	35.0	40.0	29.0	41.0
40	35.493703365278435	37.0	35.0	40.0	29.0	41.0
41	35.46562834265126	37.0	35.0	40.0	29.0	41.0
42	35.3938428093211	37.0	35.0	40.0	29.0	41.0
43	35.272247171061466	37.0	34.0	40.0	28.0	41.0
44	35.14825673293868	36.0	34.0	40.0	28.0	41.0
45	35.063074611223946	36.0	34.0	40.0	28.0	41.0
46	35.032942572439445	36.0	34.0	40.0	28.0	41.0
47	34.99134537517809	36.0	34.0	40.0	28.0	41.0
48	34.92979945175106	36.0	34.0	40.0	28.0	41.0
49	34.860473676689026	36.0	34.0	40.0	27.0	41.0
50	34.75036051914757	35.0	34.0	40.0	27.0	41.0
51	34.37928779604793	35.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	3.0
14	3.0
15	16.0
16	28.0
17	79.0
18	143.0
19	346.0
20	576.0
21	924.0
22	1395.0
23	2032.0
24	2970.0
25	4241.0
26	5808.0
27	6974.0
28	7667.0
29	8490.0
30	10095.0
31	12292.0
32	15530.0
33	20778.0
34	39707.0
35	71842.0
36	29214.0
37	38584.0
38	58862.0
39	123130.0
40	103.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.112473312921956	22.978386173386976	28.987038632928712	13.922101880762353
2	31.243260565484572	25.03778413888973	30.405297141397124	13.313658154228575
3	29.36682877397506	24.819307370180628	31.97815665368942	13.835707202154888
4	26.859217814192977	27.24658643581893	31.113127227531972	14.78106852245612
5	24.181199305378122	31.39591281715941	29.53268923466007	14.890198642802394
6	22.392028304542325	40.648154964770896	26.256187288073203	10.703629442613579
7	88.17085792730721	3.835793813361511	6.244667997592209	1.7486802617390667
8	89.4347319599683	2.5684553324354638	5.857732431999376	2.1390802755968594
9	85.19944395605347	4.528033882304031	7.601649077374121	2.6708730842683734
10	52.74470913791536	24.050849439408964	12.431956070796	10.772485351879679
11	43.865977818869986	20.733856753725362	21.317616286371294	14.082549141033358
12	38.71282755275705	21.145693041222604	24.078131969495534	16.06334743652481
13	23.50952939800881	34.63062485655019	25.201479319409138	16.658366426031865
14	17.042054071376295	36.97562327589567	28.79692703438898	17.185395618339058
15	16.170961860755163	23.10029144671029	44.36161044877597	16.36713624375858
16	19.865362879302953	20.275250414651154	40.77677260660757	19.082614099438327
17	20.144900548681992	20.080158671730537	27.577874301155823	32.197066478431644
18	24.251787438776702	22.902817895607512	31.384653360298287	21.460741305317494
19	28.89349852977477	24.087226146191057	25.620677559469417	21.398597764564755
20	30.7909335388906	24.2442089581971	24.146771350745073	20.81808615216723
21	24.970443925739552	26.54914969447897	27.734424057128752	20.74598232265273
22	24.037857758415363	23.566476266364106	27.049329412732714	25.34633656248782
23	21.086147836668587	28.15059090495719	26.467085576202702	24.296175682171516
24	22.19888531377075	23.685133619439018	34.26447598054712	19.851505086243108
25	19.85821745475647	25.301082207026766	32.53333448814942	22.30736585006734
26	19.468467024948357	31.67848187877029	26.80876678633448	22.044284309946864
27	20.057423229991727	29.579242758220488	29.38739893554827	20.975935076239516
28	17.89192653637454	27.00862214561942	35.52661778907573	19.572833528930307
29	18.796580589562485	24.24052798191558	34.959530913704924	22.00336051481701
30	21.322379902735612	25.996137140184565	31.243693621517686	21.43778933556213
31	28.292200227787472	24.511621058648778	25.251280763217952	21.944897950345794
32	29.87523655685809	25.777660371475463	25.00552146442228	19.341581607244162
33	26.48505740157719	25.718115166921447	26.043989831844343	21.752837599657017
34	21.460091721267816	25.96495710579992	28.52431826153986	24.050632911392405
35	21.769510256932143	26.520784524309597	29.114357106666034	22.59534811209222
36	28.45589540830688	24.35637047077521	26.748788525747347	20.43894559517056
37	22.69625016780921	28.628901293538373	27.84355417747502	20.831294361177395
38	22.498776616706436	30.094795965649997	24.31458056357912	23.091846854064446
39	21.89466345050386	29.159178406093964	26.021903974155215	22.924254169246957
40	24.348142406145932	25.683254156255273	25.51544494342123	24.45315849417756
41	20.105275921651504	23.907507892446205	27.188340399364275	28.79887578653802
42	23.375498555758128	26.901657305438754	24.208265307448134	25.51457883135499
43	23.399966221629416	26.69682180177293	25.560482770865722	24.34272920573193
44	21.71321297262653	29.117605026914433	26.668023575570444	22.501158424888594
45	19.88116942451184	31.42514409939502	24.462685726906205	24.231000749186936
46	23.223928944166087	29.097034865341225	26.679283032431567	20.99975315806112
47	23.13320370522742	26.019089109939937	27.441028594689865	23.40667859014278
48	24.191376122156445	23.563228346115704	28.954775958461266	23.290619573266586
49	21.8966122026529	22.770086221456197	30.338823040313184	24.99447853557772
50	19.8976255537704	29.44694414010229	26.850773221547136	23.804657084580175
51	20.126928723307508	31.328356075992676	24.519632595261502	24.025082605438318
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	254.0
1	416.0
2	578.0
3	2057.0
4	3536.0
5	2530.5
6	1525.0
7	1590.0
8	1655.0
9	1707.5
10	1760.0
11	1757.0
12	1754.0
13	1679.5
14	1605.0
15	1518.5
16	1432.0
17	1498.5
18	1565.0
19	1484.5
20	1404.0
21	1486.5
22	1569.0
23	1722.0
24	1875.0
25	1938.5
26	2738.0
27	3474.0
28	3682.0
29	3890.0
30	4973.5
31	6057.0
32	6752.0
33	7447.0
34	8567.0
35	9687.0
36	10325.0
37	10963.0
38	13371.5
39	15780.0
40	21462.0
41	27144.0
42	32298.5
43	37453.0
44	38638.0
45	39823.0
46	39119.5
47	38416.0
48	38261.5
49	38107.0
50	36348.5
51	34590.0
52	33484.5
53	32379.0
54	29803.0
55	27227.0
56	24727.5
57	22228.0
58	20998.0
59	19768.0
60	18638.5
61	17509.0
62	15626.5
63	13744.0
64	12284.5
65	10825.0
66	9409.5
67	7994.0
68	6707.5
69	5421.0
70	4592.0
71	3763.0
72	3046.0
73	2329.0
74	1953.5
75	1244.0
76	910.0
77	661.0
78	412.0
79	294.0
80	176.0
81	140.5
82	105.0
83	89.5
84	74.0
85	45.5
86	17.0
87	17.0
88	17.0
89	11.0
90	5.0
91	3.5
92	2.0
93	2.5
94	3.0
95	2.0
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	461834.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.106905798249404
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.32970520219239	19.666255175235346
2	5.439132590875086	2.839978443483281
3	2.1953334422998334	1.71940090121605
4	1.3386076742044946	1.3978761780508187
5	1.0965584946471585	1.431387466101177
6	0.9065028613898524	1.4199590884889046
7	0.773370258286041	1.4133213136169036
8	0.7068301359882736	1.4762518220487737
9	0.6710699993627322	1.5767605131654718
>10	11.393208478867397	63.881194331610025
>50	0.13138653432280847	2.100825175930656
>100	0.017462767220120114	0.6302258433116352
>500	0.0	0.0
>1k	8.315603438152434E-4	0.44656374774096924
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2057	0.445398130064049	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.165280165600627E-4	0.0	0.0	0.046986579593533605	0.0
2	2.165280165600627E-4	0.0	0.0	0.1444241870455618	0.0
3	2.165280165600627E-4	0.0	0.0	0.20656772779829982	0.0
4	2.165280165600627E-4	0.0	0.0	0.3159143761611315	0.0
5	2.165280165600627E-4	0.0	0.0	0.5690356275198448	0.0
6	2.165280165600627E-4	0.0	0.0	0.8799698593000949	0.0
7	2.165280165600627E-4	0.0	0.0	1.0941160676779969	0.0
8	2.165280165600627E-4	0.0	0.0	1.6902176972678495	0.0
9	2.165280165600627E-4	0.0	0.0	1.9530827093717655	0.0
10	2.165280165600627E-4	0.0	0.0	2.3475967555442	0.0
11	2.165280165600627E-4	0.0	0.0	2.686896157493818	0.0
12	2.165280165600627E-4	0.0	0.0	2.9991295573734287	0.0
13	2.165280165600627E-4	0.0	0.0	3.155895841362914	0.0
14	2.165280165600627E-4	0.0	0.0	3.2301649510430153	0.0
15	2.165280165600627E-4	0.0	0.0	3.2990208603091156	0.0
16	2.165280165600627E-4	0.0	0.0	3.4503739438845993	0.0
17	2.165280165600627E-4	0.0	0.0	3.608439395973445	0.0
18	2.165280165600627E-4	0.0	0.0	3.805046835009982	0.0
19	2.165280165600627E-4	0.0	0.0	3.919373627753695	0.0
20	2.165280165600627E-4	0.0	0.0	4.055136694136855	0.0
21	2.165280165600627E-4	0.0	0.0	4.216233538457541	0.0
22	2.165280165600627E-4	0.0	0.0	4.381444415092869	0.0
23	2.165280165600627E-4	0.0	0.0	4.553367660241559	0.0
24	2.165280165600627E-4	0.0	0.0	4.689347254641278	0.0
25	2.165280165600627E-4	0.0	0.0	4.802591407302191	0.0
26	2.165280165600627E-4	0.0	0.0	4.923630568559266	0.0
27	2.165280165600627E-4	0.0	0.0	5.046185425932261	0.0
28	2.165280165600627E-4	0.0	0.0	5.1698229233880575	0.0
29	2.165280165600627E-4	0.0	0.0	5.313597526383939	0.0
30	2.165280165600627E-4	0.0	0.0	5.514752053768237	0.0
31	2.165280165600627E-4	0.0	0.0	5.675199314039244	0.0
32	2.165280165600627E-4	0.0	0.0	5.821572253233846	0.0
33	2.165280165600627E-4	0.0	0.0	5.970543528627169	0.0
34	2.165280165600627E-4	0.0	0.0	6.128175924682894	0.0
35	2.165280165600627E-4	0.0	0.0	6.3087602904939875	0.0
36	2.165280165600627E-4	0.0	0.0	6.477435615394276	0.0
37	2.165280165600627E-4	0.0	0.0	6.651740668725126	0.0
38	2.165280165600627E-4	0.0	0.0	6.844883659496702	0.0
39	2.165280165600627E-4	0.0	0.0	7.1332989775547055	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGGTC	20	7.0288975E-4	45.000004	5
CGTTAGA	20	7.0288975E-4	45.000004	42
GATATAC	20	7.0288975E-4	45.000004	9
GTCGTAA	20	7.0288975E-4	45.000004	22
AACTCGT	20	7.0288975E-4	45.000004	19
AAGTGCG	20	7.0288975E-4	45.000004	1
ATACCCG	20	7.0288975E-4	45.000004	25
ACTACGG	25	3.8871694E-5	45.0	2
ATCGGGT	35	1.2098099E-7	45.0	4
AATACGG	25	3.8871694E-5	45.0	2
TACGTAG	35	1.2098099E-7	45.0	1
TAGTACG	25	3.8871694E-5	45.0	1
CGGGTAT	30	2.162671E-6	44.999996	6
GCGATAT	30	2.162671E-6	44.999996	9
GGCGATA	100	0.0	42.75	8
CGTTTTT	1430	0.0	42.639862	1
CGCAGTA	120	0.0	41.249996	37
ACGGGAT	175	0.0	41.142857	5
TAGGCGG	55	6.002665E-11	40.909092	2
AGTACGG	105	0.0	40.714287	2
>>END_MODULE