FastQCFastQC Report
Thu 26 May 2016
SRR1547077_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547077_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences853143
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40930.4797554454528725No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACG302.1648411E-645.0000041
ACGATTG302.1648411E-645.0000041
ACCCACG253.889957E-545.00000422
CGATTCG207.0322637E-445.010
TACGCGG406.8121153E-945.02
TTACGCG207.0322637E-445.01
TAGTACG207.0322637E-445.01
CGTTTTT29400.042.24491
AGTACGG800.042.18752
GCGTTAG700.041.7857171
TACACGG700.041.7857172
CGGTCTA650.041.5384631
CGTTAGG1200.041.2500042
ACGTAGG1050.040.7142872
ACGTTAG501.0804797E-940.5000041
TATAGCG501.0804797E-940.5000041
CGGGATA1350.040.06
TATGACG403.4568257E-739.3751
AATGGGC1950.039.230774
GGCGATT3400.039.044128
Produced by FastQC (version 0.11.5)