##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547077_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	853143
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.69982406232015	31.0	31.0	33.0	30.0	34.0
2	32.08860062146674	33.0	31.0	34.0	30.0	34.0
3	32.17977642669517	33.0	31.0	34.0	30.0	34.0
4	35.83458927753026	37.0	35.0	37.0	35.0	37.0
5	35.78967535336983	37.0	35.0	37.0	35.0	37.0
6	35.837446946174325	37.0	35.0	37.0	35.0	37.0
7	36.128522416523374	37.0	35.0	37.0	35.0	37.0
8	36.0650266133579	37.0	35.0	37.0	35.0	37.0
9	37.8222572300306	39.0	38.0	39.0	35.0	39.0
10	37.451724974593944	39.0	37.0	39.0	35.0	39.0
11	37.15671229793833	39.0	37.0	39.0	34.0	39.0
12	36.3936104498308	38.0	35.0	39.0	33.0	39.0
13	36.06731462369146	38.0	35.0	39.0	32.0	39.0
14	37.05201941526801	39.0	35.0	41.0	32.0	41.0
15	37.269565594513466	39.0	35.0	41.0	32.0	41.0
16	37.42653927887822	39.0	35.0	41.0	33.0	41.0
17	37.40936044719349	39.0	35.0	41.0	33.0	41.0
18	37.324053529127006	39.0	35.0	41.0	33.0	41.0
19	37.27638156792003	39.0	35.0	41.0	33.0	41.0
20	37.097685851023805	39.0	35.0	41.0	32.0	41.0
21	37.00970528973455	38.0	35.0	41.0	32.0	41.0
22	36.94496467766834	38.0	35.0	41.0	32.0	41.0
23	36.919853998684864	38.0	35.0	41.0	32.0	41.0
24	36.828376954391	38.0	35.0	41.0	32.0	41.0
25	36.72444361613469	38.0	35.0	41.0	32.0	41.0
26	36.589269325306546	38.0	35.0	41.0	32.0	41.0
27	36.49259268375876	38.0	35.0	41.0	31.0	41.0
28	36.52347730685243	38.0	35.0	41.0	31.0	41.0
29	36.538441972799404	38.0	35.0	41.0	31.0	41.0
30	36.495360097896835	38.0	35.0	41.0	31.0	41.0
31	36.348193679137026	38.0	35.0	41.0	31.0	41.0
32	36.25560544949674	38.0	35.0	41.0	31.0	41.0
33	36.1261968978237	38.0	35.0	41.0	30.0	41.0
34	35.95080777782857	38.0	35.0	41.0	30.0	41.0
35	35.89310350081991	38.0	35.0	41.0	30.0	41.0
36	35.79466513820075	38.0	35.0	41.0	30.0	41.0
37	35.711659123968666	38.0	35.0	41.0	29.0	41.0
38	35.634400094708624	38.0	35.0	40.0	29.0	41.0
39	35.58180750472078	37.0	35.0	40.0	29.0	41.0
40	35.44310156679478	37.0	35.0	40.0	29.0	41.0
41	35.401010147185175	37.0	35.0	40.0	29.0	41.0
42	35.31883048914426	37.0	35.0	40.0	28.0	41.0
43	35.209636602539085	37.0	34.0	40.0	28.0	41.0
44	35.07899027478395	36.0	34.0	40.0	27.0	41.0
45	34.99604286737394	36.0	34.0	40.0	27.0	41.0
46	34.98114852961344	36.0	34.0	40.0	27.0	41.0
47	34.93525704366091	36.0	34.0	40.0	27.0	41.0
48	34.88012560614106	36.0	34.0	40.0	27.0	41.0
49	34.81455043292859	36.0	34.0	40.0	27.0	41.0
50	34.68764322042143	35.0	34.0	40.0	27.0	41.0
51	34.31788926358184	35.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	1.0
11	5.0
12	6.0
13	5.0
14	10.0
15	21.0
16	47.0
17	141.0
18	308.0
19	609.0
20	1074.0
21	1759.0
22	2595.0
23	3747.0
24	5582.0
25	8402.0
26	11229.0
27	13210.0
28	14166.0
29	15749.0
30	18097.0
31	22340.0
32	28074.0
33	37801.0
34	72251.0
35	127853.0
36	54593.0
37	71573.0
38	110598.0
39	231111.0
40	184.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.39711396565406	23.584674550456374	27.54766785872943	13.470543625160142
2	33.39276065091081	24.30635895740808	28.921528981659584	13.379351410021531
3	30.959639825914298	24.185863331235208	31.078025606492698	13.776471236357796
4	27.181492434445335	26.651686762945953	30.911699445462247	15.255121357146455
5	25.03003599631011	30.919083905042882	28.546093679488667	15.504786419158338
6	22.747651917673824	39.494316896464014	26.552875660938437	11.205155524923724
7	85.6487130527942	4.541091001156898	7.438143429647785	2.3720525164011192
8	86.64643559168861	3.789634328594386	7.100216493600721	2.4637135861162784
9	82.12984224215636	5.117078848446274	9.164114339565582	3.5889645698317865
10	50.36928158585372	23.789564000407903	14.077475874501696	11.76367853923668
11	43.176349099740605	21.77384096218336	20.988157905532837	14.061652032543195
12	37.43897564652116	22.118214648657965	24.578997893670813	15.863811811150066
13	24.563525692644728	33.023303244590885	25.97348861796909	16.439682444795302
14	18.254384083324833	35.1851916970543	29.76476393758139	16.79566028203947
15	17.571849033514898	22.908000182853286	43.54639257428122	15.973758209350603
16	21.110177309079486	20.68680162645653	39.796376457405145	18.40664460705884
17	21.29174124384775	20.18348623853211	28.315417227826988	30.209355289793155
18	24.779198797856868	22.876000858003877	31.787402580810014	20.557397763329245
19	29.431759974588083	23.86844878291213	25.966455799320865	20.733335443178927
20	30.6576974786173	24.44092022087739	25.177959615211048	19.72342268529426
21	25.29165685002397	26.578662662648583	28.28447282577481	19.845207661552635
22	24.236616839146542	23.999259209769054	27.898839936563974	23.865284014520427
23	22.795123443549322	27.386733525329284	26.94729957345955	22.870843457661845
24	22.871077884950118	23.740920338091033	33.15493416695677	20.233067610002074
25	21.1032617040754	24.503160665914155	31.816823205488408	22.576754424522033
26	19.263945200277092	29.918431025045038	27.840584755427873	22.977039019249997
27	19.870877449618646	28.835494166863	29.505487356750276	21.78814102676808
28	18.231879063650524	27.0408360614809	35.05543619299461	19.671848681873964
29	19.933235108299545	25.363039959303425	33.62800843469383	21.075716497703198
30	21.923757213034627	25.85322741908449	31.143079178988753	21.07993618889213
31	27.806475584983993	24.70945667959533	25.78020331878712	21.703864416633554
32	29.87213163561091	25.180303888093793	26.075230061080028	18.87233441521527
33	27.494335650647077	25.841740481959064	26.292309730021813	20.371614137372045
34	20.86121552893243	26.733736313841877	30.43499155475694	21.970056602468755
35	21.863861040880604	26.64090310768535	29.700179219661887	21.795056631772162
36	28.369218290485886	25.412386903485114	26.005487942818494	20.212906863210506
37	23.408033588741866	29.252305885414287	27.011181009514228	20.32847951632962
38	22.97692180560586	30.148873049418444	24.165116516222955	22.709088628752742
39	23.06776237981206	27.880085753502048	26.37541420371497	22.67673766297092
40	24.751067523263977	24.8679295264686	26.061750492004272	24.31925245826315
41	20.415920894855844	24.28373672408963	27.033217174611995	28.26712520644253
42	23.69848899891343	25.998103483237863	25.159088218504987	25.14431929934372
43	23.189195715138027	25.503110264047173	26.455471122660562	24.852222898154235
44	22.098288329154666	27.69277835017107	27.459288771050105	22.749644549624154
45	20.253579997726057	30.844184386439323	24.45404814902074	24.448187466813888
46	22.451687466227817	29.20635813691257	26.807932550580617	21.534021846278996
47	22.445592356732693	25.632982981751006	28.253880064655046	23.667544596861255
48	23.686767634499724	23.613391893269945	29.849626615936604	22.850213856293728
49	22.779182387946687	23.263274738232628	30.507077945901212	23.45046492791947
50	20.467612111920275	29.511465252601266	26.72096002663094	23.299962608847522
51	20.157699236821962	29.736281022056094	25.99107066458964	24.114949076532305
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	414.0
1	842.0
2	1270.0
3	4371.0
4	7472.0
5	5359.5
6	3247.0
7	3221.0
8	3195.0
9	3335.0
10	3475.0
11	3515.5
12	3556.0
13	3583.5
14	3611.0
15	3404.5
16	3198.0
17	3053.0
18	2908.0
19	2880.5
20	2853.0
21	3270.0
22	3687.0
23	3726.5
24	3766.0
25	4304.0
26	5562.0
27	6282.0
28	7017.5
29	7753.0
30	9045.5
31	10338.0
32	12508.0
33	14678.0
34	16708.5
35	18739.0
36	20126.0
37	21513.0
38	25616.0
39	29719.0
40	38466.5
41	47214.0
42	56743.0
43	66272.0
44	69529.5
45	72787.0
46	69853.5
47	66920.0
48	66885.0
49	66850.0
50	64595.5
51	62341.0
52	58874.5
53	55408.0
54	51215.5
55	47023.0
56	44647.0
57	42271.0
58	41231.0
59	40191.0
60	38058.5
61	35926.0
62	32724.5
63	29523.0
64	25256.0
65	20989.0
66	18074.0
67	15159.0
68	12521.5
69	9884.0
70	8327.0
71	6770.0
72	5929.5
73	5089.0
74	4041.5
75	2251.0
76	1508.0
77	1154.5
78	801.0
79	531.5
80	262.0
81	265.0
82	268.0
83	183.5
84	99.0
85	65.0
86	31.0
87	31.5
88	32.0
89	20.0
90	8.0
91	7.0
92	6.0
93	3.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	853143.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.033362189670594
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.68645591983288	18.670667323532232
2	5.569087194662979	2.6768777923038383
3	2.0775281021756125	1.497899560164164
4	1.2373240164958346	1.1894822493768937
5	0.8473878364340123	1.018278939406998
6	0.6835723811804776	0.9857125571859591
7	0.5501368113803562	0.9255146069242217
8	0.5182202431897275	0.9963659838878317
9	0.45378491681224165	0.981537953576233
>10	9.653587249839065	59.38067208753074
>50	0.6890286719971567	9.998361531613813
>100	0.03290443408666911	1.1087782815205482
>500	4.911109565174495E-4	0.0867523990757508
>1k	4.911109565174495E-4	0.4830987339007456
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4093	0.4797554454528725	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04079034815968718	0.0
2	0.0	0.0	0.0	0.13819488643756087	0.0
3	0.0	0.0	0.0	0.20922635478460236	0.0
4	0.0	0.0	0.0	0.3213998122237421	0.0
5	0.0	0.0	0.0	0.5622738509253431	0.0
6	0.0	0.0	0.0	0.8616374980513232	0.0
7	1.1721364413703213E-4	0.0	0.0	1.0495309696029858	0.0
8	1.1721364413703213E-4	0.0	0.0	1.5195576825924846	0.0
9	1.1721364413703213E-4	0.0	0.0	1.772856367572611	0.0
10	1.1721364413703213E-4	0.0	0.0	2.145478542284236	0.0
11	1.1721364413703213E-4	0.0	0.0	2.459025040350797	0.0
12	1.1721364413703213E-4	0.0	0.0	2.7320156175459447	0.0
13	1.1721364413703213E-4	0.0	0.0	2.8622399761821877	0.0
14	1.1721364413703213E-4	0.0	0.0	2.914634475111441	0.0
15	1.1721364413703213E-4	0.0	0.0	2.994222539480486	0.0
16	1.1721364413703213E-4	0.0	0.0	3.1401535264310905	0.0
17	1.1721364413703213E-4	0.0	0.0	3.302259996272606	0.0
18	1.1721364413703213E-4	0.0	0.0	3.5233249291150486	0.0
19	1.1721364413703213E-4	0.0	0.0	3.644289409864466	0.0
20	1.1721364413703213E-4	0.0	0.0	3.7854146374054527	0.0
21	1.1721364413703213E-4	0.0	0.0	3.9486932436883384	0.0
22	1.1721364413703213E-4	0.0	0.0	4.106345595052646	0.0
23	1.1721364413703213E-4	0.0	0.0	4.27958736108718	0.0
24	1.1721364413703213E-4	0.0	0.0	4.4113354970972045	0.0
25	1.1721364413703213E-4	0.0	0.0	4.528197500301825	0.0
26	1.1721364413703213E-4	0.0	0.0	4.64529393079472	0.0
27	1.1721364413703213E-4	0.0	0.0	4.7724707346834	0.0
28	1.1721364413703213E-4	0.0	0.0	4.892849147212132	0.0
29	1.1721364413703213E-4	0.0	0.0	5.035029297550352	0.0
30	1.1721364413703213E-4	0.0	0.0	5.219875214354452	0.0
31	1.1721364413703213E-4	0.0	0.0	5.366743910458153	0.0
32	1.1721364413703213E-4	0.0	0.0	5.507517497066728	0.0
33	1.1721364413703213E-4	0.0	0.0	5.652159133931826	0.0
34	1.1721364413703213E-4	0.0	0.0	5.814265603773341	0.0
35	1.1721364413703213E-4	0.0	0.0	5.982701610398257	0.0
36	1.1721364413703213E-4	0.0	0.0	6.136134270573632	0.0
37	1.1721364413703213E-4	0.0	0.0	6.310430959405399	0.0
38	1.1721364413703213E-4	0.0	0.0	6.5137966319831495	0.0
39	1.1721364413703213E-4	0.0	0.0	6.817262756653926	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGACG	30	2.1648411E-6	45.000004	1
ACGATTG	30	2.1648411E-6	45.000004	1
ACCCACG	25	3.889957E-5	45.000004	22
CGATTCG	20	7.0322637E-4	45.0	10
TACGCGG	40	6.8121153E-9	45.0	2
TTACGCG	20	7.0322637E-4	45.0	1
TAGTACG	20	7.0322637E-4	45.0	1
CGTTTTT	2940	0.0	42.2449	1
AGTACGG	80	0.0	42.1875	2
GCGTTAG	70	0.0	41.785717	1
TACACGG	70	0.0	41.785717	2
CGGTCTA	65	0.0	41.53846	31
CGTTAGG	120	0.0	41.250004	2
ACGTAGG	105	0.0	40.714287	2
ACGTTAG	50	1.0804797E-9	40.500004	1
TATAGCG	50	1.0804797E-9	40.500004	1
CGGGATA	135	0.0	40.0	6
TATGACG	40	3.4568257E-7	39.375	1
AATGGGC	195	0.0	39.23077	4
GGCGATT	340	0.0	39.04412	8
>>END_MODULE