FastQCFastQC Report
Thu 26 May 2016
SRR1547076_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547076_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1019759
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55740.5465997358199339No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC13830.13562027890903636No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG551.8189894E-1245.01
CGCATCG253.890495E-545.021
CGTCTAT207.0329127E-445.033
TAGCACG253.890495E-545.01
AAACGCG351.2117562E-745.01
TATAGCG502.1827873E-1145.01
CGACTAT207.0329127E-445.038
GCTAACG253.890495E-545.01
AATCGAC207.0329127E-445.038
CGTTACG302.1652613E-644.99999624
ACGGGTA1150.043.043485
CGTTTTT33750.041.6666681
ATCACGG603.6379788E-1241.2499962
TACGTAG603.6379788E-1241.2499961
CGGGTAT950.040.2631576
AATACGG950.040.2631572
ATAGGGT2500.039.64
TTGCGAG1100.038.8636361
TAACGGG2900.038.7931023
ACGGGAT3200.038.6718755
Produced by FastQC (version 0.11.5)