FastQCFastQC Report
Thu 26 May 2016
SRR1547075_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547075_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences696136
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42740.6139604904788719No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTAG351.2110104E-745.0000041
ACCGCTA207.031368E-445.025
CTATACG207.031368E-445.01
CGGGCTA207.031368E-445.06
ACTCGAC207.031368E-445.035
TTGCACG207.031368E-445.01
CGTTTAG207.031368E-445.045
TATCGCG207.031368E-445.025
CGTTTTT28850.042.8162881
CGTTAGG700.041.7857172
GCGATAT501.0804797E-940.59
TTAACGG451.9264917E-840.0000042
GGCGATA1800.040.0000048
GTAGGGT2500.039.6000024
ATATCGG403.455607E-739.3752
TATTACG403.455607E-739.3751
GACCGAT1950.039.230779
TAGTAGG2550.038.823532
TTACGAG356.244738E-638.571431
TAGGGCG1750.038.5714265
Produced by FastQC (version 0.11.5)