FastQCFastQC Report
Thu 26 May 2016
SRR1547074_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547074_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1062268
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167211.574084882534351No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTAAC207.0330466E-445.018
ACCGTAA207.0330466E-445.017
CGTTTTT88650.044.1624341
TATGCGG1200.041.2500042
TCTTGCG501.0822987E-940.51
GCGATCG501.0822987E-940.59
TTATGCG403.4578989E-739.3751
ATAGGGC5650.039.026554
ATAGGGT3250.038.769234
CGATTCG356.247843E-638.57142610
TATGGGA6700.037.947764
GTTTTTT107800.037.8200382
GGGCGAT12050.037.7178427
AGGGCGA6850.037.4452556
CGTAAGG1750.037.2857132
TAGGGAC5750.036.7826085
TAGCGGG7000.036.6428573
GTAGGGA11050.036.4479644
TCGTAAG751.8189894E-1236.0000041
TACGATG250.00210703736.01
Produced by FastQC (version 0.11.5)