##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547074_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1062268
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.648111399383207	31.0	31.0	33.0	30.0	34.0
2	32.034263481531966	33.0	31.0	34.0	30.0	34.0
3	32.03400177732926	33.0	31.0	34.0	30.0	34.0
4	35.78783414354946	37.0	35.0	37.0	35.0	37.0
5	35.75834441026182	37.0	35.0	37.0	35.0	37.0
6	35.818747246457576	37.0	35.0	37.0	35.0	37.0
7	36.109747257754165	37.0	35.0	37.0	35.0	37.0
8	36.0452465856074	37.0	35.0	37.0	35.0	37.0
9	37.836939454073736	39.0	38.0	39.0	35.0	39.0
10	37.392743639081665	39.0	37.0	39.0	34.0	39.0
11	37.17585016210599	39.0	37.0	39.0	34.0	39.0
12	36.77781124913864	39.0	35.0	39.0	33.0	39.0
13	36.62218103152877	39.0	35.0	39.0	33.0	39.0
14	37.77875263116276	40.0	36.0	41.0	33.0	41.0
15	37.89688101307768	40.0	36.0	41.0	33.0	41.0
16	37.97948916845843	40.0	36.0	41.0	33.0	41.0
17	37.94995048330553	40.0	36.0	41.0	33.0	41.0
18	37.7623594045947	39.0	36.0	41.0	33.0	41.0
19	37.61857365561233	39.0	36.0	41.0	33.0	41.0
20	37.35744463732316	39.0	35.0	41.0	33.0	41.0
21	37.277911035633196	39.0	35.0	41.0	33.0	41.0
22	37.2226660315476	39.0	35.0	41.0	33.0	41.0
23	37.16522290043567	39.0	35.0	41.0	32.0	41.0
24	37.06603230070001	39.0	35.0	41.0	32.0	41.0
25	36.96050902408808	38.0	35.0	41.0	32.0	41.0
26	36.83605643773511	38.0	35.0	41.0	32.0	41.0
27	36.72633648005964	38.0	35.0	41.0	31.0	41.0
28	36.71992755123942	38.0	35.0	41.0	31.0	41.0
29	36.6917849356283	38.0	35.0	41.0	31.0	41.0
30	36.604860543666945	38.0	35.0	41.0	31.0	41.0
31	36.40833480816517	38.0	35.0	41.0	31.0	41.0
32	36.15956990138082	38.0	35.0	41.0	30.0	41.0
33	35.8574032165141	38.0	35.0	41.0	29.0	41.0
34	35.57152903033886	38.0	35.0	41.0	27.0	41.0
35	35.36279356998422	38.0	35.0	41.0	25.0	41.0
36	35.23749185704549	38.0	35.0	41.0	24.0	41.0
37	35.155539844935554	38.0	35.0	41.0	24.0	41.0
38	35.073090783116875	38.0	35.0	41.0	24.0	41.0
39	34.98881167464331	38.0	34.0	41.0	23.0	41.0
40	34.88041059318364	38.0	34.0	40.0	23.0	41.0
41	34.80814822624799	38.0	34.0	40.0	23.0	41.0
42	34.7193100046316	38.0	34.0	40.0	23.0	41.0
43	34.59234581103827	37.0	34.0	40.0	23.0	41.0
44	34.45719441798115	37.0	34.0	40.0	23.0	41.0
45	34.35746440634567	37.0	34.0	40.0	23.0	41.0
46	34.323941792466684	37.0	34.0	40.0	23.0	41.0
47	34.280211773300145	37.0	34.0	40.0	23.0	41.0
48	34.19375618958681	36.0	34.0	40.0	23.0	41.0
49	34.11098140958779	36.0	34.0	40.0	22.0	41.0
50	34.01342128351791	36.0	33.0	40.0	22.0	41.0
51	33.6499414460381	36.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	1.0
12	3.0
13	6.0
14	16.0
15	34.0
16	83.0
17	179.0
18	418.0
19	829.0
20	1469.0
21	2475.0
22	3745.0
23	5564.0
24	8527.0
25	13698.0
26	20007.0
27	23609.0
28	23630.0
29	22672.0
30	24000.0
31	27575.0
32	33020.0
33	42416.0
34	71807.0
35	112251.0
36	75873.0
37	98383.0
38	149374.0
39	300396.0
40	205.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.47943833382913	22.05413323191511	27.085537736239818	19.380890698015943
2	36.2219326949508	22.8389634254256	28.041793596342917	12.897310283280678
3	27.877804847740872	23.6516585268501	35.36150952490332	13.10902710050571
4	24.920547357164104	25.584033407765272	35.29627175063167	14.199147484438956
5	22.77946808150109	29.821193898338272	33.320687434809294	14.078650585351344
6	20.86272014218634	38.519187248415655	29.98292333008243	10.635169279315578
7	82.50912199181374	3.4319964453414773	12.12754220215614	1.9313393606886398
8	83.38893763155814	2.600473703434538	11.823005117352684	2.187583547654641
9	79.2764161209789	3.8220110179352105	13.597886785632252	3.3036860754536517
10	41.491412713175954	22.69897991843866	21.810880116881993	13.998727251503388
11	32.45489838722432	23.29995820263813	27.704402278897604	16.54074113123995
12	29.340147684011946	20.452936547086047	31.683624094861184	18.523291674040827
13	22.541298429398232	25.376176256839138	32.78617072151284	19.29635459224979
14	18.570454913449336	28.08754476271525	33.87864456050639	19.46335576332903
15	17.639898782604764	23.20356068336804	41.252960646465866	17.90357988756133
16	20.39137016270847	21.403449976842	38.40885727518856	19.79632258526097
17	20.97879254576058	21.86444475405453	32.30286519032862	24.85389750985627
18	22.681093659980345	22.98901972007064	34.03858536640471	20.291301253544304
19	25.53583464813023	23.48155079509126	29.997138198646667	20.985476358131848
20	26.47034458347611	22.858167618717687	30.323986037421818	20.347501760384386
21	24.10201568719005	24.381512010151866	31.76561846916221	19.750853833495878
22	23.56392172220193	21.75289098419608	31.793389238873804	22.889798054728185
23	21.930435633945482	24.8942827986911	30.872246928270453	22.30303463909296
24	20.773665402704403	22.997398020085328	35.455647727315515	20.77328884989475
25	20.964954230005986	24.17393727383297	33.31042637074636	21.550682125414678
26	20.544156465223466	26.45885972278182	31.077091656719396	21.919892155275317
27	20.403137438009995	25.985721117458116	32.56146283235493	21.049678612176965
28	19.83228337858243	24.33124221006375	35.42015762500612	20.416316786347704
29	20.44531135269066	23.45679244785685	34.80760034190995	21.29029585754254
30	22.068159824074527	24.207168059284477	32.763012723719434	20.96165939292156
31	25.280155290378698	23.682253442634064	29.441534528009882	21.596056738977357
32	26.66615204449348	24.50266787665636	29.093317317287166	19.737862761562997
33	24.60339575323741	24.943046387540623	29.716041526243846	20.737516332978117
34	22.022314519499787	24.709395369153548	31.12237213207966	22.145917979267
35	22.58027164519688	25.25925660944319	30.40372109486495	21.75675065049498
36	25.370339688289583	25.353677226462622	28.649549831116065	20.626433254131726
37	22.57980095418482	26.426005490139964	29.171263748884463	21.822929806790754
38	22.120500664615708	27.654791446226373	27.812567073469218	22.412140815688698
39	21.792240752804375	26.57229625668852	28.30858126197909	23.32688172852802
40	23.328952768981086	24.983808229185104	28.60690522542334	23.080333776410473
41	20.817439666826072	24.399586545015005	29.66643069357262	25.1165430945863
42	22.839151701830424	26.001818750070605	27.599061630398353	23.559967917700618
43	22.673562603787367	25.715073785523053	28.371371442988018	23.23999216770156
44	22.38324038754815	26.754830231165773	28.81391513252776	22.048014248758317
45	20.822993820768392	27.578821916879733	28.10307756611326	23.49510669623861
46	22.618209340768995	26.80999521777932	28.535454329792483	22.036341111659205
47	22.636566290239372	25.19825505428009	29.878429925404888	22.28674873007565
48	22.52567148779781	24.38480684723629	30.44702466797456	22.642496996991344
49	21.951616729488226	24.042991034277602	30.461521951146036	23.543870285088133
50	20.79842374993881	26.36858118666852	30.754950728064856	22.07804433532781
51	20.8060489443342	26.980667778752633	28.82587068423411	23.38741259267906
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	578.0
1	1077.5
2	1577.0
3	14274.0
4	26971.0
5	18447.0
6	9923.0
7	9637.0
8	9351.0
9	9332.5
10	9314.0
11	9203.5
12	9093.0
13	8606.0
14	8119.0
15	7532.0
16	6945.0
17	6419.5
18	5894.0
19	5466.5
20	5039.0
21	4873.0
22	4707.0
23	4944.0
24	5181.0
25	5554.5
26	6567.5
27	7207.0
28	8687.5
29	10168.0
30	11822.5
31	13477.0
32	16016.5
33	18556.0
34	21052.5
35	23549.0
36	26643.5
37	29738.0
38	32350.5
39	34963.0
40	42358.0
41	49753.0
42	55763.5
43	61774.0
44	66724.5
45	71675.0
46	75238.0
47	78801.0
48	78299.0
49	77797.0
50	76404.5
51	75012.0
52	70414.0
53	65816.0
54	62404.0
55	58992.0
56	56128.0
57	53264.0
58	52580.5
59	51897.0
60	48792.0
61	45687.0
62	41862.0
63	38037.0
64	33497.0
65	28957.0
66	25194.5
67	21432.0
68	17802.0
69	14172.0
70	11797.5
71	9423.0
72	7818.0
73	6213.0
74	4966.0
75	2738.0
76	1757.0
77	1345.0
78	933.0
79	731.0
80	529.0
81	368.5
82	208.0
83	141.5
84	75.0
85	53.5
86	32.0
87	26.0
88	20.0
89	11.5
90	3.0
91	5.0
92	7.0
93	4.5
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1062268.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.630315290134533
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.30678974003077	22.017336081948454
2	5.806100911301176	3.440732012163826
3	2.6750156670915954	2.377846728659206
4	1.8159093433004754	2.15223865521177
5	1.4365726440281654	2.1283050189868375
6	1.237507640695503	2.2000644940654968
7	1.0539051418101393	2.1859279146409865
8	0.9493711023650742	2.250413207233579
9	0.8757858745443381	2.3354830430455453
>10	9.749595226472003	54.467466807310586
>50	0.07232531052707686	1.3272498546164575
>100	0.019201270757977354	1.2089020530658363
>500	0.0016001058964295414	0.3224939903043302
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	3.2002117928590825E-4	1.5855401387470462
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16721	1.574084882534351	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.039538045013122865	0.0
2	0.0	0.0	0.0	0.15090353846675228	0.0
3	0.0	0.0	0.0	0.2578445364070084	0.0
4	0.0	0.0	0.0	0.39876942541806776	0.0
5	0.0	0.0	0.0	0.7626135777412103	0.0
6	0.0	0.0	0.0	1.332149702335004	0.0
7	0.0	0.0	0.0	1.7194342670587837	0.0
8	0.0	0.0	0.0	2.4364849548324905	0.0
9	0.0	0.0	0.0	2.9032221623921646	0.0
10	0.0	0.0	0.0	3.4634386049471506	0.0
11	0.0	0.0	0.0	3.808078563978205	0.0
12	0.0	0.0	0.0	4.113368754400961	0.0
13	0.0	0.0	0.0	4.274909909740291	0.0
14	0.0	0.0	0.0	4.360293259328154	0.0
15	0.0	0.0	0.0	4.4366393414844465	0.0
16	0.0	0.0	0.0	4.5654204023843326	0.0
17	0.0	0.0	0.0	4.703521145323026	0.0
18	0.0	0.0	0.0	4.914296580523936	0.0
19	0.0	0.0	0.0	5.026980008811336	0.0
20	0.0	0.0	0.0	5.151430712400261	0.0
21	0.0	0.0	0.0	5.2923556014113196	0.0
22	0.0	0.0	0.0	5.436575327506806	0.0
23	0.0	0.0	0.0	5.588231971592856	0.0
24	0.0	0.0	0.0	5.717860276314452	0.0
25	0.0	0.0	0.0	5.83948683383101	0.0
26	0.0	0.0	0.0	5.952170262118411	0.0
27	0.0	0.0	0.0	6.0734202668253205	0.0
28	0.0	0.0	0.0	6.19890649064078	0.0
29	0.0	0.0	0.0	6.329852730196146	0.0
30	0.0	0.0	0.0	6.5079622091600235	0.0
31	0.0	0.0	0.0	6.653123317279632	0.0
32	0.0	0.0	0.0	6.807509969235635	0.0
33	0.0	0.0	0.0	6.952388662748007	0.0
34	0.0	0.0	0.0	7.099526673118271	0.0
35	0.0	0.0	0.0	7.274435453200134	0.0
36	0.0	0.0	0.0	7.430422454597145	0.0
37	0.0	0.0	0.0	7.583208757112141	0.0
38	0.0	0.0	0.0	7.752657521454096	0.0
39	0.0	0.0	0.0	7.9751060937541185	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTAAC	20	7.0330466E-4	45.0	18
ACCGTAA	20	7.0330466E-4	45.0	17
CGTTTTT	8865	0.0	44.162434	1
TATGCGG	120	0.0	41.250004	2
TCTTGCG	50	1.0822987E-9	40.5	1
GCGATCG	50	1.0822987E-9	40.5	9
TTATGCG	40	3.4578989E-7	39.375	1
ATAGGGC	565	0.0	39.02655	4
ATAGGGT	325	0.0	38.76923	4
CGATTCG	35	6.247843E-6	38.571426	10
TATGGGA	670	0.0	37.94776	4
GTTTTTT	10780	0.0	37.820038	2
GGGCGAT	1205	0.0	37.717842	7
AGGGCGA	685	0.0	37.445255	6
CGTAAGG	175	0.0	37.285713	2
TAGGGAC	575	0.0	36.782608	5
TAGCGGG	700	0.0	36.642857	3
GTAGGGA	1105	0.0	36.447964	4
TCGTAAG	75	1.8189894E-12	36.000004	1
TACGATG	25	0.002107037	36.0	1
>>END_MODULE