FastQCFastQC Report
Thu 26 May 2016
SRR1547073_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547073_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences910271
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44950.4938089865545535No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC12770.1402878922870222No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATAA700.045.0000049
ATGCGCG351.2115561E-745.0000041
ATAAGTC207.032514E-445.029
GACGATA207.032514E-445.01
CGTTTTT26250.043.1999971
GGCGATA2950.041.9491548
CGCAGTA2200.041.9318237
CTCGCGC2150.041.86046633
TATAGCG700.041.7857171
AATACGG700.041.7857172
GGGCGAT12050.041.078847
TAACGGG2400.040.3124963
ATTGCGG1350.040.0000042
TAAACGG451.927583E-840.02
ATCCGCG451.927583E-840.01
AGGGCGA6600.039.8863646
CGTTAGG1300.039.8076932
GCGAGAC2550.039.70588321
TCGATAG403.4571713E-739.3751
TAACACG403.4571713E-739.3751
Produced by FastQC (version 0.11.5)