##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547073_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	910271
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.677353227775026	31.0	31.0	33.0	30.0	34.0
2	32.06014252898313	33.0	31.0	34.0	30.0	34.0
3	32.14948185760065	33.0	31.0	34.0	30.0	34.0
4	35.81835848884563	37.0	35.0	37.0	35.0	37.0
5	35.75126857825856	37.0	35.0	37.0	35.0	37.0
6	35.80558427105774	37.0	35.0	37.0	35.0	37.0
7	36.115609527272646	37.0	35.0	37.0	35.0	37.0
8	36.06245942142505	37.0	35.0	37.0	35.0	37.0
9	37.84752342983573	39.0	38.0	39.0	35.0	39.0
10	37.430965064250096	39.0	37.0	39.0	35.0	39.0
11	37.13768756776828	39.0	37.0	39.0	33.0	39.0
12	36.280311028254225	38.0	35.0	39.0	33.0	39.0
13	35.88105959653773	38.0	35.0	39.0	31.0	39.0
14	36.74631730550572	39.0	35.0	41.0	31.0	41.0
15	37.018195680187546	39.0	35.0	41.0	32.0	41.0
16	37.225591060244696	39.0	35.0	41.0	33.0	41.0
17	37.20348116110477	39.0	35.0	41.0	33.0	41.0
18	37.13663733108052	39.0	35.0	41.0	32.0	41.0
19	37.11813734591127	38.0	35.0	41.0	32.0	41.0
20	36.922164937694376	38.0	35.0	41.0	32.0	41.0
21	36.835188641624306	38.0	35.0	41.0	32.0	41.0
22	36.79800960373339	38.0	35.0	41.0	32.0	41.0
23	36.77070564699963	38.0	35.0	41.0	32.0	41.0
24	36.69931042513713	38.0	35.0	41.0	32.0	41.0
25	36.598162525226	38.0	35.0	41.0	32.0	41.0
26	36.45306727337243	38.0	35.0	41.0	31.0	41.0
27	36.38621245760878	38.0	35.0	40.0	31.0	41.0
28	36.42498772343621	38.0	35.0	40.0	31.0	41.0
29	36.43432889765795	38.0	35.0	40.0	31.0	41.0
30	36.391784424638374	38.0	35.0	40.0	31.0	41.0
31	36.214140624055915	38.0	35.0	40.0	31.0	41.0
32	36.11714203792057	38.0	35.0	40.0	31.0	41.0
33	36.00224218941392	38.0	35.0	41.0	30.0	41.0
34	35.85621314971036	38.0	35.0	41.0	30.0	41.0
35	35.77278744461814	38.0	35.0	41.0	30.0	41.0
36	35.6730347336123	37.0	35.0	40.0	30.0	41.0
37	35.58405793439536	37.0	35.0	40.0	29.0	41.0
38	35.49749689927505	37.0	35.0	40.0	29.0	41.0
39	35.44449949520527	37.0	35.0	40.0	29.0	41.0
40	35.305332148338245	37.0	34.0	40.0	28.0	41.0
41	35.283346388053666	37.0	34.0	40.0	28.0	41.0
42	35.20747777310274	36.0	34.0	40.0	28.0	41.0
43	35.092862455246845	36.0	34.0	40.0	28.0	41.0
44	34.906976054383804	36.0	34.0	40.0	27.0	41.0
45	34.83228730784568	36.0	34.0	40.0	27.0	41.0
46	34.8406562441295	35.0	34.0	40.0	27.0	41.0
47	34.794219523636364	35.0	34.0	40.0	27.0	41.0
48	34.75464120025794	35.0	34.0	40.0	27.0	41.0
49	34.69480297625652	36.0	34.0	40.0	27.0	41.0
50	34.57128042088565	35.0	34.0	40.0	27.0	41.0
51	34.21008908336089	35.0	34.0	39.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	4.0
13	5.0
14	8.0
15	29.0
16	60.0
17	166.0
18	379.0
19	693.0
20	1203.0
21	1833.0
22	2869.0
23	4255.0
24	6076.0
25	8959.0
26	12107.0
27	14144.0
28	15469.0
29	17368.0
30	20337.0
31	24789.0
32	31701.0
33	42459.0
34	81601.0
35	142604.0
36	56833.0
37	76228.0
38	115315.0
39	232592.0
40	183.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.02352596095009	23.13300105133526	28.305416738531715	13.538056249182937
2	32.27829953936794	24.298917575095768	29.83518095160672	13.587601933929566
3	29.087271812460248	25.202494641705602	31.603775139491425	14.106458406342725
4	26.976911271478492	27.60342799012602	30.4212701492193	14.998390589176191
5	24.70473078896285	31.545550720609576	28.891396078750176	14.858322411677403
6	22.075074345991467	39.940413349431104	26.643384222940202	11.341128081637226
7	86.5523563861751	4.451421609608567	6.979679677810234	2.016542326406092
8	87.67443980968305	3.1690562480843614	6.8076429986234865	2.3488609436091012
9	83.82064242406932	4.443731592020398	8.437267582950572	3.2983584009597138
10	51.82961997031653	23.345135679374604	13.180031001756621	11.645213348552245
11	45.5342420004592	20.89586507754284	19.566261036548457	14.003631885449497
12	39.16822572618484	21.02297008253586	23.659657398730708	16.149146792548592
13	24.397459657618445	33.4346584698403	25.889652641905542	16.278229230635713
14	17.545983558742396	36.0249859657179	30.34041510714941	16.088615368390293
15	17.175434568386777	22.475174975364478	44.64285910459632	15.70653135165242
16	21.786259256858674	19.799158712075855	40.070374646671155	18.344207384394316
17	21.81657989763488	19.13111589845222	28.142937652633115	30.90936655127978
18	25.09615268420064	22.264029063872186	31.901488677547675	20.7383295743795
19	29.803761736889346	23.843998106058525	25.09109924407127	21.261140912980856
20	30.50904620711854	25.05012243606574	24.34417882147185	20.09665253534387
21	24.646066940504532	26.719735111851307	28.71166938197526	19.922528565668905
22	24.07535777806829	23.18792974839361	28.311788467390482	24.42492400614762
23	21.783732536793988	27.97595441357574	27.267264364128923	22.97304868550135
24	22.45529078702936	23.91101111646971	34.002181767847155	19.631516328653774
25	21.171497279381633	24.30715687965452	31.829861656583592	22.691484184380258
26	19.113099285817082	30.476088988883532	27.39316093778666	23.017650787512732
27	20.04479984532079	28.160295120903555	29.838256958641985	21.95664807513367
28	17.316161890250267	26.767962507868535	35.036159561273514	20.879716040607686
29	19.074429483088004	26.098930977697847	33.489916739080996	21.33672280013315
30	22.49681688200547	26.261959350567025	30.3021847339968	20.939039033430703
31	28.9816988567141	24.166319700396915	25.57150562854359	21.280475814345397
32	30.40556054186061	25.93996732841099	25.222708402223077	18.431763727505327
33	28.167216136732907	25.351680982916076	25.224136548346593	21.256966332004428
34	20.92223085213085	26.82333063450335	30.182550031803718	22.071888481562084
35	20.784030250332044	27.78304482950682	29.962176099205625	21.470748820955517
36	29.578004791979534	25.604682561566833	25.955567078375562	18.861745568078078
37	23.551008435949296	29.427500162039657	26.470578541994637	20.550912860016414
38	23.642409787854383	29.72916856628411	24.07942250165061	22.5489991442109
39	22.699833346333126	27.866866021217856	25.9222802879582	23.511020344490817
40	25.73024956304221	24.513688780593913	24.9557549345195	24.800306721844372
41	20.01425948975635	23.652516668113122	26.526276240811804	29.80694760131873
42	23.039072979365486	24.94839448911368	25.98786515224587	26.024667379274963
43	22.69038561043909	25.520751512461676	26.081134079850944	25.70772879724829
44	22.785082684167683	27.91641170596449	27.094678397971595	22.203827211896236
45	20.077097919191097	30.82466650041581	23.932762880504814	25.165472699888277
46	23.13377005309408	28.568964627017667	26.328423074007627	21.968842245880623
47	21.84063866694644	26.34248482045457	28.4316428843718	23.385233628227198
48	24.38339791117151	23.590996527407775	29.06815662588394	22.957448935536778
49	23.004797472401076	23.236267001804958	29.84748497974779	23.91145054604618
50	20.36613272311213	29.279961681740936	27.003167188672382	23.350738406474555
51	19.955815356086266	31.04460100343744	24.224763834066998	24.7748198064093
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	393.0
1	812.0
2	1231.0
3	4454.0
4	7677.0
5	5416.0
6	3155.0
7	3197.0
8	3239.0
9	3364.0
10	3489.0
11	3504.0
12	3519.0
13	3383.5
14	3248.0
15	3211.0
16	3174.0
17	3035.0
18	2896.0
19	2693.0
20	2490.0
21	2600.5
22	2711.0
23	3270.5
24	3830.0
25	4343.5
26	5531.5
27	6206.0
28	7071.0
29	7936.0
30	8994.5
31	10053.0
32	12573.0
33	15093.0
34	17350.5
35	19608.0
36	21005.0
37	22402.0
38	26370.0
39	30338.0
40	40848.0
41	51358.0
42	61148.0
43	70938.0
44	72331.0
45	73724.0
46	73466.5
47	73209.0
48	72727.0
49	72245.0
50	71034.5
51	69824.0
52	65820.5
53	61817.0
54	57440.0
55	53063.0
56	51356.0
57	49649.0
58	46157.0
59	42665.0
60	40204.0
61	37743.0
62	34845.5
63	31948.0
64	27744.5
65	23541.0
66	19527.5
67	15514.0
68	12582.5
69	9651.0
70	7937.5
71	6224.0
72	5244.0
73	4264.0
74	3429.5
75	1900.0
76	1205.0
77	1037.5
78	870.0
79	607.5
80	345.0
81	299.5
82	254.0
83	144.0
84	34.0
85	33.5
86	33.0
87	19.5
88	6.0
89	4.5
90	3.0
91	3.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	910271.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.662332895827312
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.95477537909346	18.445918458395525
2	6.246254365598386	2.956019003016073
3	2.156046007017865	1.5305123507032785
4	1.3213509254636335	1.2506498188212
5	0.8819395182427591	1.0434373237322867
6	0.664768013708444	0.9437977223320259
7	0.540817204691974	0.8957897713248588
8	0.4590860730339879	0.8690437990374651
9	0.3730709910404088	0.7944956985396925
>10	8.091897397912623	50.58839884289657
>50	1.2571642398170113	18.263533498144973
>100	0.05189484217805497	1.7798681368354177
>500	0.0	0.0
>1k	9.35042201406396E-4	0.6385355762206496
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4495	0.4938089865545535	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1277	0.1402878922870222	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.034165649570292805	0.0
2	0.0	0.0	0.0	0.13512459476353744	0.0
3	0.0	0.0	0.0	0.20752061748644085	0.0
4	0.0	0.0	0.0	0.33484533726769283	0.0
5	0.0	0.0	0.0	0.6271758630122238	0.0
6	0.0	0.0	0.0	0.9771815206680209	0.0
7	0.0	0.0	0.0	1.213594632807153	0.0
8	0.0	0.0	0.0	1.8217651666371883	0.0
9	0.0	0.0	0.0	2.1572696482695815	0.0
10	0.0	0.0	0.0	2.6217467105949765	0.0
11	0.0	0.0	0.0	2.989768980885912	0.0
12	0.0	0.0	0.0	3.304949844606716	0.0
13	0.0	0.0	0.0	3.443809590770221	0.0
14	0.0	0.0	0.0	3.5042311575344045	0.0
15	0.0	0.0	0.0	3.5811313334160926	0.0
16	0.0	0.0	0.0	3.7283402415324667	0.0
17	0.0	0.0	0.0	3.8837884542075933	0.0
18	0.0	0.0	0.0	4.140525184258315	0.0
19	0.0	0.0	0.0	4.273452631139518	0.0
20	0.0	0.0	0.0	4.429999417755811	0.0
21	1.0985739411669711E-4	0.0	0.0	4.6013769525778585	0.0
22	1.0985739411669711E-4	0.0	0.0	4.780005075411609	0.0
23	1.0985739411669711E-4	0.0	0.0	4.96555421407471	0.0
24	1.0985739411669711E-4	0.0	0.0	5.110236402126399	0.0
25	1.0985739411669711E-4	0.0	0.0	5.238220266272352	0.0
26	1.0985739411669711E-4	0.0	0.0	5.3620295494418695	0.0
27	1.0985739411669711E-4	0.0	0.0	5.501987869546542	0.0
28	1.0985739411669711E-4	0.0	0.0	5.635244888610095	0.0
29	1.0985739411669711E-4	0.0	0.0	5.8004704093616075	0.0
30	1.0985739411669711E-4	0.0	0.0	6.007661454665699	0.0
31	1.0985739411669711E-4	0.0	0.0	6.16926168141136	0.0
32	1.0985739411669711E-4	0.0	0.0	6.328994332457038	0.0
33	1.0985739411669711E-4	0.0	0.0	6.484772117314514	0.0
34	1.0985739411669711E-4	0.0	0.0	6.658127085230662	0.0
35	1.0985739411669711E-4	0.0	0.0	6.854332391123084	0.0
36	1.0985739411669711E-4	0.0	0.0	7.017141049204028	0.0
37	1.0985739411669711E-4	0.0	0.0	7.204887335749464	0.0
38	1.0985739411669711E-4	0.0	0.0	7.490736275241109	0.0
39	1.0985739411669711E-4	0.0	0.0	8.037386668365794	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATAA	70	0.0	45.000004	9
ATGCGCG	35	1.2115561E-7	45.000004	1
ATAAGTC	20	7.032514E-4	45.0	29
GACGATA	20	7.032514E-4	45.0	1
CGTTTTT	2625	0.0	43.199997	1
GGCGATA	295	0.0	41.949154	8
CGCAGTA	220	0.0	41.93182	37
CTCGCGC	215	0.0	41.860466	33
TATAGCG	70	0.0	41.785717	1
AATACGG	70	0.0	41.785717	2
GGGCGAT	1205	0.0	41.07884	7
TAACGGG	240	0.0	40.312496	3
ATTGCGG	135	0.0	40.000004	2
TAAACGG	45	1.927583E-8	40.0	2
ATCCGCG	45	1.927583E-8	40.0	1
AGGGCGA	660	0.0	39.886364	6
CGTTAGG	130	0.0	39.807693	2
GCGAGAC	255	0.0	39.705883	21
TCGATAG	40	3.4571713E-7	39.375	1
TAACACG	40	3.4571713E-7	39.375	1
>>END_MODULE