FastQCFastQC Report
Thu 26 May 2016
SRR1547072_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547072_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1138103
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53690.47174992070137767No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC13660.12002428602683587No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGA207.033259E-445.035
CGACGAA207.033259E-445.035
TCGGACG253.8907812E-545.01
ATCGGTT207.033259E-445.033
CATACGA302.1654832E-644.99999618
ATACGAA302.1654832E-644.99999619
CGTTTTT38150.042.640891
TAAACGG603.6379788E-1241.2499962
GACCGAT2750.040.9090929
ATATACG403.45819E-739.3751
CGGGATC1750.038.5714266
TATAGCG356.248236E-638.5714261
GGGCGAT14050.038.274027
CGTAAGG1600.037.968752
TAGGGTG3350.037.611945
TTTACGG900.037.5000042
TCGTTAG301.139915E-437.4999961
CGACTTA301.139915E-437.49999615
TAGATAC1850.036.48648520
AGTGCGG1550.036.290322