##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547072_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1138103
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.686616237721893	31.0	31.0	33.0	30.0	34.0
2	32.076111740325786	33.0	31.0	34.0	30.0	34.0
3	32.16725902664346	33.0	31.0	34.0	30.0	34.0
4	35.84716936867753	37.0	35.0	37.0	35.0	37.0
5	35.79036343810709	37.0	35.0	37.0	35.0	37.0
6	35.84046786626518	37.0	35.0	37.0	35.0	37.0
7	36.128771297501196	37.0	35.0	37.0	35.0	37.0
8	36.05968001138737	37.0	35.0	37.0	35.0	37.0
9	37.81201789293236	39.0	38.0	39.0	35.0	39.0
10	37.42376920190879	39.0	37.0	39.0	35.0	39.0
11	37.158506743238526	39.0	37.0	39.0	34.0	39.0
12	36.51272687972881	39.0	35.0	39.0	33.0	39.0
13	36.248522321793374	39.0	35.0	39.0	32.0	39.0
14	37.313871415856035	40.0	35.0	41.0	32.0	41.0
15	37.48792508235195	40.0	35.0	41.0	33.0	41.0
16	37.61188134993054	40.0	35.0	41.0	33.0	41.0
17	37.588458162398304	39.0	35.0	41.0	33.0	41.0
18	37.503906061226445	39.0	35.0	41.0	33.0	41.0
19	37.441552302383876	39.0	35.0	41.0	33.0	41.0
20	37.28327313081505	39.0	35.0	41.0	33.0	41.0
21	37.19829224595665	39.0	35.0	41.0	32.0	41.0
22	37.103016159345856	39.0	35.0	41.0	32.0	41.0
23	37.05763010904988	39.0	35.0	41.0	32.0	41.0
24	36.970778567493454	38.0	35.0	41.0	32.0	41.0
25	36.865905809931085	38.0	35.0	41.0	32.0	41.0
26	36.73138020021035	38.0	35.0	41.0	32.0	41.0
27	36.644388952493756	38.0	35.0	41.0	31.0	41.0
28	36.662606108585955	38.0	35.0	41.0	31.0	41.0
29	36.68447583390959	38.0	35.0	41.0	31.0	41.0
30	36.63656452886953	38.0	35.0	41.0	31.0	41.0
31	36.511805170533776	38.0	35.0	41.0	31.0	41.0
32	36.39598876375864	38.0	35.0	41.0	31.0	41.0
33	36.289946516264344	38.0	35.0	41.0	30.0	41.0
34	36.14081590154846	38.0	35.0	41.0	30.0	41.0
35	36.05298993149126	38.0	35.0	41.0	30.0	41.0
36	35.95619465022059	38.0	35.0	41.0	30.0	41.0
37	35.88115750507643	38.0	35.0	41.0	30.0	41.0
38	35.81704204276766	38.0	35.0	41.0	30.0	41.0
39	35.74910003751857	38.0	35.0	41.0	29.0	41.0
40	35.625521591630985	38.0	35.0	40.0	29.0	41.0
41	35.60758384785911	38.0	35.0	40.0	29.0	41.0
42	35.52580302485803	38.0	35.0	40.0	29.0	41.0
43	35.405346440524276	37.0	35.0	40.0	28.0	41.0
44	35.20966555751105	37.0	34.0	40.0	28.0	41.0
45	35.14009891899064	37.0	34.0	40.0	27.0	41.0
46	35.10046103032854	37.0	34.0	40.0	27.0	41.0
47	35.07432016258634	37.0	34.0	40.0	27.0	41.0
48	35.009365584661495	36.0	34.0	40.0	27.0	41.0
49	34.94757592239015	36.0	34.0	40.0	27.0	41.0
50	34.84911734702395	36.0	34.0	40.0	27.0	41.0
51	34.47935116593138	35.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	3.0
12	7.0
13	4.0
14	16.0
15	28.0
16	74.0
17	169.0
18	377.0
19	752.0
20	1353.0
21	2177.0
22	3336.0
23	4887.0
24	7197.0
25	10373.0
26	14527.0
27	17263.0
28	18463.0
29	20062.0
30	23810.0
31	28882.0
32	36316.0
33	48345.0
34	90172.0
35	156882.0
36	75746.0
37	99907.0
38	154491.0
39	322249.0
40	233.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.923025420370564	23.35623401396886	27.880692696530986	13.840047869129595
2	33.21491991498134	24.349114271731118	29.62860127774024	12.807364535547311
3	29.818654374867652	24.88149139401267	31.465869082148103	13.833985148971578
4	27.093593462103165	27.564464727709183	30.14428395320986	15.197657856977795
5	24.552083598760394	31.29031379409421	28.887719301328612	15.269883305816784
6	23.112231493986044	39.68656615438146	26.273544661599168	10.927657690033328
7	86.23516500703363	4.359710852181217	7.240469447844351	2.164654692940797
8	86.48022191313089	3.6991379514859375	7.285456588727031	2.535183546656146
9	81.89241219819296	5.3464405242759225	8.994177152683017	3.7669701248481027
10	48.40959034463489	25.213710885570112	14.423914179999525	11.952784589795476
11	40.39924330223187	22.75136784631971	22.12049348784776	14.728895363600659
12	35.557062937185826	22.196848615634966	25.79309605545368	16.45299239172553
13	24.284708853240875	31.597755211962365	26.708478933804763	17.409057000992
14	18.33542306803514	34.24189199044375	29.58572290908644	17.836962032434673
15	17.8172801582985	23.485923506044706	41.75184495603649	16.9449513796203
16	20.68969153055567	21.261959594166786	38.66996220904435	19.378386666233197
17	21.715082026846428	20.882380592969177	28.271167020911115	29.131370359273284
18	24.334177135109915	23.278912365576755	31.323439091189464	21.063471408123867
19	28.08884608862291	24.50771151644447	26.37125110820374	21.03219128672888
20	30.09850602274135	24.44198811531118	24.770868717506236	20.688637144441234
21	25.901434228712162	26.061964514635317	27.61771122648829	20.41889003016423
22	24.689505255675453	23.743193717967532	27.4775657387776	24.08973528757942
23	22.706732167475177	27.35534481501235	26.995447687950914	22.94247532956156
24	22.22619569582015	24.145793482663695	33.30287329002735	20.325137531488803
25	20.92007489656033	25.373450381907436	31.08611434993142	22.62036037160081
26	20.032193922694166	29.97066170636577	27.36140753517037	22.6357368357697
27	20.758226627994127	28.197711454938613	29.589325394977433	21.454736522089828
28	19.06953940021246	26.121537330101052	34.74017729502514	20.068745974661343
29	20.102486330323355	24.758303949642517	33.30190676942245	21.837302950611676
30	21.743638317445786	27.072769336343022	30.04763189271973	21.135960453491467
31	27.44971237225453	25.28479408278513	25.56077964823922	21.704713896721124
32	28.362371419809985	26.47695331617613	25.622285504914753	19.538389759099132
33	26.63695640904206	26.355171719958566	26.396380643931174	20.6114912270682
34	21.900829714006555	26.689148521706734	28.853451752609388	22.556570011677326
35	22.363705218244746	26.557086660873402	29.383017178585767	21.696190942296084
36	27.69916255382861	25.501734025830704	26.596450409145746	20.202653011194947
37	23.045717303266926	28.65812672490978	27.104488785285692	21.1916671865376
38	23.429074521374606	29.341368927065474	24.97744053042651	22.25211602113341
39	23.519751727216253	27.96337414100481	26.08498527813388	22.431888853645056
40	24.131383539099712	25.210899189264946	26.521237532982518	24.136479738652827
41	20.221983423292972	25.30482741895944	26.743625137619354	27.729564020128233
42	23.038688062504008	26.323100808977745	25.51948285875707	25.11872826976117
43	22.915939945681544	26.733081276474973	26.108445369180117	24.242533408663363
44	21.70761345853583	28.17952329446456	27.54750668436864	22.565356562630974
45	21.37346092576858	30.365177844184576	24.962503393805306	23.298857836241535
46	23.180502994895892	28.99869343987319	26.657429072764067	21.16337449246685
47	22.863396370978727	26.355347450977636	27.71032147353974	23.070934704503898
48	23.11706409701055	24.467117651038613	29.220114523905128	23.195703728045704
49	22.173564255607797	23.550680386573095	30.083744617139224	24.192010740679887
50	21.027358683704374	28.57307291167847	27.69714164710927	22.702426757507887
51	20.676863166163344	29.791416066911342	25.753292979633652	23.77842778729166
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	435.0
1	995.0
2	1555.0
3	5822.5
4	10090.0
5	7232.5
6	4375.0
7	4367.5
8	4360.0
9	4507.5
10	4655.0
11	4553.0
12	4451.0
13	4344.0
14	4237.0
15	4162.5
16	4088.0
17	3989.5
18	3891.0
19	3803.0
20	3715.0
21	3841.0
22	3967.0
23	4522.5
24	5078.0
25	5611.0
26	7308.5
27	8473.0
28	9778.5
29	11084.0
30	13338.0
31	15592.0
32	17625.0
33	19658.0
34	22403.5
35	25149.0
36	27983.5
37	30818.0
38	35717.5
39	40617.0
40	53530.5
41	66444.0
42	76047.5
43	85651.0
44	88481.0
45	91311.0
46	91883.5
47	92456.0
48	90731.5
49	89007.0
50	86821.5
51	84636.0
52	80245.0
53	75854.0
54	69801.5
55	63749.0
56	60043.5
57	56338.0
58	54437.0
59	52536.0
60	49578.5
61	46621.0
62	42284.0
63	37947.0
64	33602.0
65	29257.0
66	24806.5
67	20356.0
68	16483.0
69	12610.0
70	10319.0
71	8028.0
72	6782.5
73	5537.0
74	4578.0
75	2811.5
76	2004.0
77	1373.0
78	742.0
79	666.0
80	590.0
81	375.0
82	160.0
83	132.0
84	104.0
85	90.5
86	77.0
87	47.0
88	17.0
89	14.5
90	12.0
91	8.5
92	5.0
93	4.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1138103.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.12846806461348
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.58925168546277	18.17648997829097
2	5.751971401563833	2.660685737392783
3	2.0043495734306593	1.3907260529823793
4	1.1256405276537218	1.0413736398429507
5	0.7859731587628325	0.9089177551044775
6	0.616968284385494	0.8561718757373559
7	0.5229445365888281	0.8466434209841164
8	0.4584921541540983	0.8483376916183123
9	0.389233020856927	0.8102127143324223
>10	8.405885319612947	51.56625372855355
>50	1.3010740643664904	18.632519411666266
>100	0.047450935492149654	1.665583860435648
>500	0.0	0.0
>1k	3.8266883461410956E-4	0.12089842995669764
>5k	3.8266883461410956E-4	0.47518570310213
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5369	0.47174992070137767	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1366	0.12002428602683587	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04138465499168353	0.0
2	0.0	0.0	0.0	0.13434636408128262	0.0
3	0.0	0.0	0.0	0.210262164320804	0.0
4	0.0	0.0	0.0	0.327211157513863	0.0
5	0.0	0.0	0.0	0.6296442413384378	0.0
6	0.0	0.0	0.0	0.9782945831792026	0.0
7	0.0	0.0	0.0	1.2028788255544534	0.0
8	0.0	0.0	0.0	1.7088084294655228	0.0
9	0.0	0.0	0.0	1.975831712946895	0.0
10	0.0	0.0	0.0	2.3722808919755067	0.0
11	0.0	0.0	0.0	2.693956522388571	0.0
12	0.0	0.0	0.0	2.977498521662802	0.0
13	0.0	0.0	0.0	3.104288451923947	0.0
14	0.0	0.0	0.0	3.1562169680600087	0.0
15	0.0	0.0	0.0	3.230551189127873	0.0
16	0.0	0.0	0.0	3.3793953622826756	0.0
17	0.0	0.0	0.0	3.5448461167398735	0.0
18	0.0	0.0	0.0	3.779622758221356	0.0
19	0.0	0.0	0.0	3.9077306711255484	0.0
20	0.0	0.0	0.0	4.045064462531071	0.0
21	1.7573101907296615E-4	0.0	0.0	4.202958783168132	0.0
22	1.7573101907296615E-4	0.0	0.0	4.369288192720694	0.0
23	1.7573101907296615E-4	0.0	0.0	4.547479446060682	0.0
24	1.7573101907296615E-4	0.0	0.0	4.686482682147398	0.0
25	1.7573101907296615E-4	0.0	0.0	4.809933723046156	0.0
26	1.7573101907296615E-4	0.0	0.0	4.932242512320941	0.0
27	1.7573101907296615E-4	0.0	0.0	5.051915336309631	0.0
28	1.7573101907296615E-4	0.0	0.0	5.1769479563800465	0.0
29	1.7573101907296615E-4	0.0	0.0	5.319026485300539	0.0
30	1.7573101907296615E-4	0.0	0.0	5.5007323590219865	0.0
31	1.7573101907296615E-4	0.0	0.0	5.65704510048739	0.0
32	1.7573101907296615E-4	0.0	0.0	5.802989711827489	0.0
33	1.7573101907296615E-4	0.0	0.0	5.953503329663484	0.0
34	1.7573101907296615E-4	0.0	0.0	6.10849808848584	0.0
35	1.7573101907296615E-4	0.0	0.0	6.281768873291784	0.0
36	1.7573101907296615E-4	0.0	0.0	6.444232200424742	0.0
37	1.7573101907296615E-4	0.0	0.0	6.605729006952798	0.0
38	1.7573101907296615E-4	0.0	0.0	6.7826022776497386	0.0
39	1.7573101907296615E-4	0.0	0.0	6.987328914869744	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCGA	20	7.033259E-4	45.0	35
CGACGAA	20	7.033259E-4	45.0	35
TCGGACG	25	3.8907812E-5	45.0	1
ATCGGTT	20	7.033259E-4	45.0	33
CATACGA	30	2.1654832E-6	44.999996	18
ATACGAA	30	2.1654832E-6	44.999996	19
CGTTTTT	3815	0.0	42.64089	1
TAAACGG	60	3.6379788E-12	41.249996	2
GACCGAT	275	0.0	40.909092	9
ATATACG	40	3.45819E-7	39.375	1
CGGGATC	175	0.0	38.571426	6
TATAGCG	35	6.248236E-6	38.571426	1
GGGCGAT	1405	0.0	38.27402	7
CGTAAGG	160	0.0	37.96875	2
TAGGGTG	335	0.0	37.61194	5
TTTACGG	90	0.0	37.500004	2
TCGTTAG	30	1.139915E-4	37.499996	1
CGACTTA	30	1.139915E-4	37.499996	15
TAGATAC	185	0.0	36.486485	20
AGTGCGG	155	0.0	36.29032	2
>>END_MODULE