FastQCFastQC Report
Thu 26 May 2016
SRR1547071_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547071_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences869691
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101921.1719104831486127No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATA207.032337E-445.00000430
TTATCGG502.1827873E-1145.02
TTACGCG253.890021E-545.01
CGTTTTT52500.043.071431
CGTAAGG1200.041.2500042
GCGTAAG603.6379788E-1241.2500041
ATTAGCG501.0804797E-940.51
ACGGGAC1250.039.65
ACGGGTA800.039.3750045
GGCGATT3400.039.0441178
CGGACGG356.246535E-638.5714262
CGGTAGT950.037.89473712
GGGCGAT10600.037.5707557
ATTCGCG301.1396932E-437.5000041
GCGATAT301.1396932E-437.5000049
TCGTAAG301.1396932E-437.5000041
TAGTACG301.1396932E-437.5000041
GCGATTT1150.037.1739169
AGGGCGA5650.037.0353976
GTAGGGT3600.036.8754
Produced by FastQC (version 0.11.5)