##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547071_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	869691
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.720214420984004	31.0	31.0	33.0	30.0	34.0
2	32.10724843651366	33.0	31.0	34.0	30.0	34.0
3	32.13808122655058	33.0	31.0	34.0	30.0	34.0
4	35.85074698944798	37.0	35.0	37.0	35.0	37.0
5	35.815081448468476	37.0	35.0	37.0	35.0	37.0
6	35.87023322076462	37.0	35.0	37.0	35.0	37.0
7	36.161868985651225	37.0	35.0	37.0	35.0	37.0
8	36.109091619897185	37.0	35.0	37.0	35.0	37.0
9	37.87853272024201	39.0	38.0	39.0	35.0	39.0
10	37.47532284455053	39.0	37.0	39.0	35.0	39.0
11	37.219757362097575	39.0	37.0	39.0	34.0	39.0
12	36.6125807901887	39.0	35.0	39.0	33.0	39.0
13	36.37973717101821	39.0	35.0	39.0	32.0	39.0
14	37.40153686769209	40.0	35.0	41.0	33.0	41.0
15	37.57061761016269	40.0	35.0	41.0	33.0	41.0
16	37.66836612084062	40.0	35.0	41.0	33.0	41.0
17	37.6294396515544	40.0	35.0	41.0	33.0	41.0
18	37.4742109553853	39.0	35.0	41.0	33.0	41.0
19	37.38781360276236	39.0	35.0	41.0	33.0	41.0
20	37.18579357495938	39.0	35.0	41.0	32.0	41.0
21	37.141390447871714	39.0	35.0	41.0	33.0	41.0
22	37.08032968031174	39.0	35.0	41.0	32.0	41.0
23	37.03398103464334	38.0	35.0	41.0	32.0	41.0
24	36.96231995041917	38.0	35.0	41.0	32.0	41.0
25	36.85944893071217	38.0	35.0	41.0	32.0	41.0
26	36.740366405999374	38.0	35.0	41.0	32.0	41.0
27	36.637092944505575	38.0	35.0	41.0	32.0	41.0
28	36.64589607113331	38.0	35.0	41.0	32.0	41.0
29	36.64475888562719	38.0	35.0	41.0	32.0	41.0
30	36.550257505251864	38.0	35.0	41.0	31.0	41.0
31	36.365115885987095	38.0	35.0	41.0	31.0	41.0
32	36.19922133263423	38.0	35.0	41.0	30.0	41.0
33	36.00515010503731	38.0	35.0	41.0	30.0	41.0
34	35.771516550131025	38.0	35.0	41.0	29.0	41.0
35	35.63368943682296	38.0	35.0	41.0	29.0	41.0
36	35.5375219474503	38.0	35.0	41.0	28.0	41.0
37	35.484372035585054	38.0	35.0	41.0	28.0	41.0
38	35.393452387112205	38.0	35.0	41.0	27.0	41.0
39	35.304648432604225	38.0	35.0	40.0	27.0	41.0
40	35.183971088582034	38.0	35.0	40.0	26.0	41.0
41	35.13150877725537	37.0	34.0	40.0	26.0	41.0
42	35.05020978715429	37.0	34.0	40.0	26.0	41.0
43	34.94516098246389	37.0	34.0	40.0	26.0	41.0
44	34.78925503425929	37.0	34.0	40.0	25.0	41.0
45	34.70179868482024	36.0	34.0	40.0	24.0	41.0
46	34.66582153891439	36.0	34.0	40.0	24.0	41.0
47	34.6302882288077	36.0	34.0	40.0	24.0	41.0
48	34.55210528797009	36.0	34.0	40.0	24.0	41.0
49	34.483613145358525	36.0	34.0	40.0	24.0	41.0
50	34.38281987510506	36.0	34.0	40.0	24.0	41.0
51	34.03603923692438	35.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	3.0
13	11.0
14	7.0
15	22.0
16	53.0
17	133.0
18	317.0
19	553.0
20	1016.0
21	1752.0
22	2772.0
23	4041.0
24	6210.0
25	9422.0
26	13381.0
27	16027.0
28	16818.0
29	17163.0
30	19093.0
31	22453.0
32	27933.0
33	36895.0
34	69263.0
35	111754.0
36	58631.0
37	76957.0
38	118081.0
39	238730.0
40	197.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.31505097787605	22.2155915146874	27.81585643636648	16.65350107107007
2	34.42280074187269	23.861463439313503	28.569112477880076	13.146623340933733
3	29.027206214621053	24.012321617678005	33.60480906436884	13.355663103332102
4	26.11030814392698	25.851135633230655	33.55962060088008	14.478935621962282
5	23.593897142778296	30.757245964371254	31.397013422008506	14.251843470841944
6	21.97148182515399	38.994769406605336	28.382954405645222	10.65079436259545
7	84.45401872619126	3.8833332758416494	9.738976257084413	1.9236717408826813
8	85.39998689189609	2.797085401596659	9.587543161881634	2.21538454462562
9	80.80134208586728	4.499644126477105	11.259631294333275	3.4393824933223414
10	46.80949900596879	23.695542439786085	18.36617833230423	11.128780221940897
11	39.19415056612061	20.810264795197376	24.63886598803483	15.356718650647183
12	34.61884738372594	19.976750363059985	28.568192610938826	16.836209642275247
13	25.243563518537044	26.659008774380787	29.51416077664366	18.583266930438512
14	18.897516474242	29.365027348793998	33.235712454193504	18.5017437227705
15	19.441617769989573	21.836491351526004	41.6558294842651	17.066061394219325
16	24.12673006849559	19.862571878977704	37.80457656799944	18.206121484527262
17	23.637590822487528	19.75448751338119	30.50888189023458	26.099039773896703
18	25.57667033463609	22.328045248254842	32.246510542250064	19.848773874859003
19	28.10618943969755	23.81650494256006	28.178284011217773	19.899021606524617
20	28.90842839583254	24.405449751693418	27.554729208420003	19.13139264405404
21	26.059255528687775	24.55538806311667	30.15116863345717	19.234187774738384
22	24.387167396236133	22.4232514766739	30.55188566973787	22.637695457352095
23	23.35703140540721	24.384292812044738	30.218778853638824	22.039896928909233
24	24.05107101257803	22.262964662161618	33.204436978191104	20.481527347069246
25	22.575604438817926	23.16799874898096	31.642042978483165	22.614353833717953
26	20.67044502012784	26.53551663751838	29.83611420608009	22.95792413627369
27	21.127848856662883	24.927474240851062	31.85694689263198	22.087730009854077
28	19.47588281355102	25.31899260771929	34.46867910556738	20.736445473162306
29	21.756807877740485	24.252521872711114	33.15924851470235	20.831421734846053
30	23.987255243529024	24.012551584413313	31.61076750248077	20.389425669576898
31	27.920031367462695	22.36506989264003	28.958216194027536	20.75668254586974
32	29.107349621877194	23.79454311933779	28.075948814004054	19.022158444780963
33	27.692364299504074	24.109137613244243	28.434926887825675	19.763571199426003
34	22.820059078454303	24.7783408130014	31.599039198979867	20.802560909564434
35	23.18271662004091	26.000728994550936	30.24350027768483	20.57305410772332
36	26.372930155652984	26.24012436601046	27.43089212145463	19.956053356881927
37	24.47214010493382	28.790915394088245	26.827459407996635	19.9094850929813
38	24.387972279809723	29.00501442466347	25.065454282038104	21.541559013488698
39	24.208253276163603	27.114802843768647	26.35694746754882	22.31999641251893
40	24.754079322426012	24.463171402256663	27.16206100787521	23.620688267442112
41	20.828547150654657	24.493182061214846	27.346609312962876	27.331661475167618
42	22.80304154004123	25.763058373606256	26.458362797821295	24.975537288531214
43	22.93251281202174	24.700267106363064	28.030760350515298	24.336459731099897
44	22.439004198042753	26.38385357558029	28.302581031653773	22.874561194723185
45	22.112796384003055	27.73099871103645	26.31681827223692	23.839386632723578
46	23.649089159253116	27.059035910455552	28.272915322798557	21.018959607492775
47	23.029788741058606	25.08051710320102	28.921766466480626	22.96792768925975
48	23.16420429784832	23.463965937327167	30.232001940919247	23.139827823905275
49	23.160294863348017	22.714734313681525	30.623175357684513	23.501795465285944
50	21.108301684161386	26.78618037900818	29.016397778061403	23.089120158769035
51	21.223170068449598	27.730768744301137	27.760204486421042	23.285856700828226
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	440.0
1	900.5
2	1361.0
3	9180.0
4	16999.0
5	11466.5
6	5934.0
7	5734.0
8	5534.0
9	5613.0
10	5692.0
11	5500.0
12	5308.0
13	5047.5
14	4787.0
15	4504.0
16	4221.0
17	3990.5
18	3760.0
19	3760.0
20	3760.0
21	3605.5
22	3451.0
23	3620.0
24	3789.0
25	4237.5
26	5314.0
27	5942.0
28	6717.5
29	7493.0
30	8898.5
31	10304.0
32	12300.0
33	14296.0
34	15314.5
35	16333.0
36	18793.5
37	21254.0
38	24414.0
39	27574.0
40	34273.0
41	40972.0
42	47068.0
43	53164.0
44	56137.0
45	59110.0
46	60039.0
47	60968.0
48	61882.0
49	62796.0
50	62281.5
51	61767.0
52	58997.0
53	56227.0
54	53208.0
55	50189.0
56	49421.0
57	48653.0
58	47971.0
59	47289.0
60	45118.0
61	42947.0
62	39837.5
63	36728.0
64	32113.5
65	27499.0
66	22847.5
67	18196.0
68	15139.0
69	12082.0
70	9970.0
71	7858.0
72	6312.5
73	4767.0
74	3770.0
75	2083.5
76	1394.0
77	1080.0
78	766.0
79	564.0
80	362.0
81	256.5
82	151.0
83	102.0
84	53.0
85	38.0
86	23.0
87	21.5
88	20.0
89	14.0
90	8.0
91	7.5
92	7.0
93	5.0
94	3.0
95	1.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	869691.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.61027495101874
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.41912714100039	20.54732561977842
2	5.804174514091482	3.205097083955229
3	2.4682499191400993	2.0444717674586372
4	1.6578512102920422	1.8309491097616994
5	1.3315992689384835	1.8382910969983541
6	1.095052972643794	1.8140828196375338
7	0.9709973347577462	1.8766652372557435
8	0.880347979698808	1.9445319817646367
9	0.7765985282555637	1.9297889002523352
>10	10.46895841944113	58.68131811311539
>50	0.10272430504107438	1.7751428899038837
>100	0.02347984095113171	1.2355614925813585
>500	4.19282874127352E-4	0.09689543420693311
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	4.19282874127352E-4	1.1798784533298234
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10192	1.1719104831486127	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04748813084187372	0.0
2	0.0	0.0	0.0	0.16764575004225638	0.0
3	0.0	0.0	0.0	0.28159426738922216	0.0
4	1.1498336765586858E-4	0.0	0.0	0.3908284666622973	0.0
5	1.1498336765586858E-4	0.0	0.0	0.6563250625796978	0.0
6	1.1498336765586858E-4	0.0	0.0	1.044738878521222	0.0
7	1.1498336765586858E-4	0.0	0.0	1.3288627799988733	0.0
8	1.1498336765586858E-4	0.0	0.0	1.9263163583387664	0.0
9	1.1498336765586858E-4	0.0	0.0	2.315994991324505	0.0
10	2.2996673531173716E-4	0.0	0.0	2.754311588828676	0.0
11	2.2996673531173716E-4	0.0	0.0	3.08316402032446	0.0
12	2.2996673531173716E-4	0.0	0.0	3.336127429167371	0.0
13	2.2996673531173716E-4	0.0	0.0	3.452605580602766	0.0
14	2.2996673531173716E-4	0.0	0.0	3.522515468137534	0.0
15	2.2996673531173716E-4	0.0	0.0	3.58886087127497	0.0
16	2.2996673531173716E-4	0.0	0.0	3.706258889651612	0.0
17	2.2996673531173716E-4	0.0	0.0	3.8319357104994762	0.0
18	2.2996673531173716E-4	0.0	0.0	4.00188112789485	0.0
19	2.2996673531173716E-4	0.0	0.0	4.1008818074465525	0.0
20	2.2996673531173716E-4	0.0	0.0	4.2175899256172595	0.0
21	2.2996673531173716E-4	0.0	0.0	4.354305149760087	0.0
22	2.2996673531173716E-4	0.0	0.0	4.504933361389275	0.0
23	2.2996673531173716E-4	0.0	0.0	4.663725392122029	0.0
24	2.2996673531173716E-4	0.0	0.0	4.788022412558023	0.0
25	2.2996673531173716E-4	0.0	0.0	4.894497011007358	0.0
26	2.2996673531173716E-4	0.0	0.0	5.005685927530583	0.0
27	2.2996673531173716E-4	0.0	0.0	5.135272182878746	0.0
28	2.2996673531173716E-4	0.0	0.0	5.259339236579429	0.0
29	2.2996673531173716E-4	0.0	0.0	5.399159011648965	0.0
30	2.2996673531173716E-4	0.0	0.0	5.581637616118829	0.0
31	2.2996673531173716E-4	0.0	0.0	5.721687357923677	0.0
32	2.2996673531173716E-4	0.0	0.0	5.863231883508051	0.0
33	2.2996673531173716E-4	0.0	0.0	6.006731126342575	0.0
34	2.2996673531173716E-4	0.0	0.0	6.1671329242225115	0.0
35	2.2996673531173716E-4	0.0	0.0	6.340412859279905	0.0
36	2.2996673531173716E-4	0.0	0.0	6.500699673792186	0.0
37	2.2996673531173716E-4	0.0	0.0	6.682488378056115	0.0
38	2.2996673531173716E-4	0.0	0.0	6.91360494704441	0.0
39	2.2996673531173716E-4	0.0	0.0	7.246251829672838	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCATA	20	7.032337E-4	45.000004	30
TTATCGG	50	2.1827873E-11	45.0	2
TTACGCG	25	3.890021E-5	45.0	1
CGTTTTT	5250	0.0	43.07143	1
CGTAAGG	120	0.0	41.250004	2
GCGTAAG	60	3.6379788E-12	41.250004	1
ATTAGCG	50	1.0804797E-9	40.5	1
ACGGGAC	125	0.0	39.6	5
ACGGGTA	80	0.0	39.375004	5
GGCGATT	340	0.0	39.044117	8
CGGACGG	35	6.246535E-6	38.571426	2
CGGTAGT	95	0.0	37.894737	12
GGGCGAT	1060	0.0	37.570755	7
ATTCGCG	30	1.1396932E-4	37.500004	1
GCGATAT	30	1.1396932E-4	37.500004	9
TCGTAAG	30	1.1396932E-4	37.500004	1
TAGTACG	30	1.1396932E-4	37.500004	1
GCGATTT	115	0.0	37.173916	9
AGGGCGA	565	0.0	37.035397	6
GTAGGGT	360	0.0	36.875	4
>>END_MODULE