FastQCFastQC Report
Thu 26 May 2016
SRR1547070_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547070_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences999371
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52510.5254304957818469No Hit
TGAATGATACCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCC36160.3618275895538293No Hit
TGAATCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCGTCTT16800.16810573850952248No Hit
TGAATGATACGGCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATG12640.12647955564049787No Hit
TGAACTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCGTCTTC11470.11477219170858469No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAG302.1652158E-645.0000041
CGTATCC207.032846E-445.023
CGTATCA207.032846E-445.036
GTCGACG253.8904393E-545.01
ACCGCGA253.8904393E-545.032
ACTCGTC207.032846E-445.014
CGTTTTT35500.042.464791
CGTAAGG1350.041.6666642
GACGGGT1700.041.029414
CGATTGA9650.040.56994620
TTCGATA501.0804797E-940.515
TCAACGG501.0804797E-940.52
ATTAGCG950.040.2631571
ATAGGGC4550.040.0549434
TAACGGG2650.039.9056633
ATTGAAT10050.039.85074622
CGTTAGG850.039.7058832
TTAACGG850.039.7058832
ATATCGG403.457626E-739.3752
GCGATAA800.039.3759