##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547070_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	999371
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.56034245540445	31.0	31.0	33.0	30.0	34.0
2	31.94098187760101	31.0	31.0	34.0	30.0	34.0
3	32.056417486599074	33.0	31.0	34.0	30.0	34.0
4	35.75763755402148	37.0	35.0	37.0	35.0	37.0
5	35.70004933102922	37.0	35.0	37.0	33.0	37.0
6	35.730838697540754	37.0	35.0	37.0	33.0	37.0
7	36.02248714441384	37.0	35.0	37.0	35.0	37.0
8	35.95897119288032	37.0	35.0	37.0	35.0	37.0
9	37.707260867085395	39.0	38.0	39.0	35.0	39.0
10	37.22801241981206	39.0	37.0	39.0	34.0	39.0
11	37.01663346244788	39.0	37.0	39.0	33.0	39.0
12	36.42484222576	38.0	35.0	39.0	32.0	39.0
13	36.15353957639355	39.0	35.0	39.0	32.0	39.0
14	37.19822268206702	39.0	35.0	41.0	32.0	41.0
15	37.394715275908545	39.0	35.0	41.0	32.0	41.0
16	37.484660851675706	39.0	35.0	41.0	33.0	41.0
17	37.47957165056821	39.0	35.0	41.0	33.0	41.0
18	37.32022442116091	39.0	35.0	41.0	32.0	41.0
19	37.2995083907778	39.0	35.0	41.0	32.0	41.0
20	37.15008040057196	39.0	35.0	41.0	32.0	41.0
21	36.965809494171836	39.0	35.0	41.0	32.0	41.0
22	36.93651506797776	39.0	35.0	41.0	32.0	41.0
23	36.87911296205313	38.0	35.0	41.0	32.0	41.0
24	36.851073325121504	38.0	35.0	41.0	32.0	41.0
25	36.67570501845661	38.0	35.0	41.0	31.0	41.0
26	36.55776083156305	38.0	35.0	41.0	31.0	41.0
27	36.47211896282762	38.0	35.0	41.0	31.0	41.0
28	36.48774178958565	38.0	35.0	41.0	31.0	41.0
29	36.48160092698307	38.0	35.0	41.0	31.0	41.0
30	36.42286698333252	38.0	35.0	41.0	31.0	41.0
31	36.29094800629596	38.0	35.0	41.0	30.0	41.0
32	36.191747609246214	38.0	35.0	41.0	30.0	41.0
33	36.06566830536407	38.0	35.0	41.0	30.0	41.0
34	35.88889411439796	38.0	35.0	41.0	30.0	41.0
35	35.86746163336739	38.0	35.0	41.0	29.0	41.0
36	35.73589487787819	38.0	35.0	41.0	29.0	41.0
37	35.64499169977916	38.0	35.0	41.0	29.0	41.0
38	35.54593739462122	38.0	35.0	41.0	28.0	41.0
39	35.49855859335522	38.0	35.0	41.0	28.0	41.0
40	35.35136801047859	38.0	35.0	40.0	27.0	41.0
41	35.31062138084855	38.0	35.0	40.0	27.0	41.0
42	35.22319739115904	38.0	35.0	40.0	27.0	41.0
43	35.147564818270695	37.0	34.0	40.0	27.0	41.0
44	35.02259821427678	37.0	34.0	40.0	26.0	41.0
45	34.916460453625334	37.0	34.0	40.0	26.0	41.0
46	34.91527170590301	37.0	34.0	40.0	26.0	41.0
47	34.86972805894908	37.0	34.0	40.0	26.0	41.0
48	34.82873927700523	36.0	34.0	40.0	26.0	41.0
49	34.7726049685252	36.0	34.0	40.0	26.0	41.0
50	34.62512020060618	36.0	34.0	40.0	26.0	41.0
51	34.27875633773644	36.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	2.0
13	6.0
14	10.0
15	31.0
16	91.0
17	235.0
18	569.0
19	1255.0
20	1948.0
21	2854.0
22	4425.0
23	6350.0
24	8401.0
25	10874.0
26	13728.0
27	15699.0
28	16823.0
29	18582.0
30	21808.0
31	25746.0
32	32740.0
33	42624.0
34	76626.0
35	132269.0
36	65606.0
37	86173.0
38	134111.0
39	279574.0
40	208.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.95983173416079	22.075285354487974	30.919848584759812	14.045034326591427
2	33.58682611362547	23.817981510370025	29.57009959264377	13.025092783360734
3	28.417574654457656	26.92063307820619	31.226841683418872	13.434950583917285
4	25.17223333476757	29.366271384700976	31.085152560960843	14.376342719570609
5	23.931753072682717	30.942662934986103	31.122375974487955	14.003208017843225
6	23.754941858428953	39.41018900888659	25.63302317157492	11.20184596110954
7	85.62055532930214	5.785839292915243	6.76925786319595	1.8243475145866752
8	86.68092230012678	2.6705797946908607	8.623824385538503	2.024673519643856
9	82.04570674954546	6.23712315046164	8.65744553324041	3.0597245667524873
10	44.38271672882243	24.448978407418267	17.770077378671186	13.398227485088121
11	40.92824386539134	22.189357105619433	21.439285310460278	15.443113718528956
12	36.22438513825196	21.50542691352861	25.330733031076548	16.939454917142882
13	23.26103118861764	30.559822128118586	27.170690364239107	19.008456319024667
14	19.913225418788418	33.003359112882	29.693277071277834	17.390138397051746
15	17.959396460373576	24.97240764440833	40.07090459899277	16.997291296225324
16	20.277154330073614	21.53544579540531	38.546545777293915	19.640854097227155
17	20.831503015396684	20.66649922801442	28.015721889068224	30.486275867520668
18	23.854804672138773	24.293080347538602	31.37433445637306	20.477780523949566
19	27.333092515192053	24.606577537270944	26.52908679559443	21.531243151942572
20	29.141930274142435	24.853732998055776	24.78138749273293	21.222949235068857
21	26.05608928015722	26.08300621090666	28.266979930376205	19.593924578559914
22	23.790564264922637	24.283774494156823	27.77897297400065	24.14668826691989
23	21.360635839943324	26.992878520589453	28.54845697944007	23.098028660027158
24	21.965116058000483	24.44567633041183	34.20551526910426	19.38369234248342
25	21.46930419233698	24.786690828531146	31.57546096494695	22.16854401418492
26	20.05321347127343	30.949167026059392	27.265249842150713	21.732369660516465
27	20.615367065884442	28.820327986303383	29.74801149923302	20.816293448579156
28	18.38336313541217	26.761833193078445	34.670607812313946	20.184195859195434
29	20.36510965397235	24.974609029079293	33.00015709881515	21.660124218133205
30	22.096398634741252	26.830676495515682	30.42733879610275	20.64558607364032
31	26.992878520589453	24.874045774792343	26.966561967477542	21.16651373714066
32	28.09537198898107	25.80953419700992	26.699093729956143	19.39600008405287
33	25.91259902478659	25.060563094186243	26.60463431498413	22.42220356604304
34	21.865553433109426	25.583992331176308	29.257102717609378	23.29335151810489
35	23.05440121836635	25.577388177163435	29.506159374246398	21.862051230223813
36	27.086837620863523	24.79229435314813	26.683383848440666	21.437484177547677
37	23.424834220724836	27.970593503313584	28.01472125967233	20.589851016289245
38	22.271708904901182	29.872589859021325	25.582791575901243	22.27290966017625
39	22.834863128908083	27.720536217280667	26.57671675483879	22.867883898972455
40	24.139183546450717	25.412684578599936	25.744893538035424	24.70323833691392
41	20.097941605269714	24.952094867671764	27.01209060499054	27.93787292206798
42	22.902605739009836	25.917301982947272	25.758902349577884	25.421189928465004
43	22.15043262211931	25.62481801052862	26.8751044406932	25.349644926658872
44	21.34602665076333	28.026828875362604	27.41204217452778	23.215102299346288
45	20.625073171024574	29.76512226190274	25.554273638118374	24.05553092895431
46	23.311362847230907	27.956584691771123	26.86910066431786	21.86295179668011
47	22.69997828634211	25.5794894988948	28.952411066560867	22.76812114820222
48	23.339980847953363	22.762817812404002	31.019411209650873	22.877790129991766
49	22.13092034889946	23.433139444710722	30.311766100877453	24.124174105512367
50	20.699820186897558	28.359638212435623	28.098273814229152	22.84226778643767
51	20.82690012017559	28.529745209736923	26.802058494793222	23.84129617529426
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	421.0
1	799.5
2	1178.0
3	5270.0
4	9362.0
5	6621.5
6	3881.0
7	3846.5
8	3812.0
9	4049.0
10	4286.0
11	4253.0
12	4220.0
13	4036.5
14	3853.0
15	3609.5
16	3366.0
17	3418.5
18	3471.0
19	3325.0
20	3179.0
21	3204.5
22	3230.0
23	3636.0
24	4042.0
25	4834.0
26	6476.5
27	7327.0
28	8504.0
29	9681.0
30	11863.0
31	14045.0
32	16314.0
33	18583.0
34	19861.0
35	21139.0
36	23979.5
37	26820.0
38	31312.0
39	35804.0
40	45919.5
41	56035.0
42	65933.0
43	75831.0
44	80284.5
45	84738.0
46	84026.5
47	83315.0
48	82613.0
49	81911.0
50	78767.5
51	75624.0
52	71913.0
53	68202.0
54	62920.0
55	57638.0
56	53082.0
57	48526.0
58	47170.5
59	45815.0
60	42653.0
61	39491.0
62	34888.0
63	30285.0
64	26377.5
65	22470.0
66	18961.0
67	15452.0
68	12861.0
69	10270.0
70	8265.0
71	6260.0
72	5379.5
73	4499.0
74	3697.5
75	2093.0
76	1290.0
77	1021.0
78	752.0
79	608.0
80	464.0
81	302.5
82	141.0
83	110.0
84	79.0
85	53.0
86	27.0
87	18.5
88	10.0
89	8.0
90	6.0
91	7.5
92	9.0
93	8.5
94	8.0
95	4.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	999371.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.401864065436268
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.89823880574141	19.008622342745944
2	5.794509901963798	2.827936859070901
3	2.100115468674198	1.5374019656492326
4	1.2147717318909432	1.1857077868854957
5	0.8561998297363979	1.0446435929038629
6	0.7133118151020701	1.0443682769034195
7	0.5698715021606885	0.9734148851343765
8	0.517725329382428	1.010677048865858
9	0.4628197695694805	1.0164298593447902
>10	9.015413478328048	55.21087409814414
>50	0.8157399799232988	12.116008397163194
>100	0.037979796525110625	1.521426695356539
>500	0.0012384716258188248	0.1971417627499667
>1k	0.0016512955010917665	0.7763779077741406
>5k	4.128238752729416E-4	0.528968521308163
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5251	0.5254304957818469	No Hit
TGAATGATACCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCC	3616	0.3618275895538293	No Hit
TGAATCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCGTCTT	1680	0.16810573850952248	No Hit
TGAATGATACGGCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATG	1264	0.12647955564049787	No Hit
TGAACTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCGTCTTC	1147	0.11477219170858469	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0006293958900148E-4	0.0	0.0	0.05323348386134879	0.0
2	1.0006293958900148E-4	0.0	0.0	0.14329012949145012	0.0
3	1.0006293958900148E-4	0.0	0.0	0.2436532578992186	0.0
4	1.0006293958900148E-4	0.0	0.0	0.3476186521321912	0.0
5	1.0006293958900148E-4	0.0	0.0	0.7326608436706689	0.0
6	1.0006293958900148E-4	0.0	0.0	1.2627942976131987	0.0
7	1.0006293958900148E-4	0.0	0.0	1.5268603951885735	0.0
8	1.0006293958900148E-4	0.0	0.0	2.1307402356081977	0.0
9	1.0006293958900148E-4	0.0	0.0	2.421523138053836	0.0
10	1.0006293958900148E-4	0.0	0.0	2.84599012779038	0.0
11	1.0006293958900148E-4	0.0	0.0	3.5639417193414658	0.0
12	1.0006293958900148E-4	0.0	0.0	3.858927265249842	0.0
13	1.0006293958900148E-4	0.0	0.0	4.13570135615302	0.0
14	1.0006293958900148E-4	0.0	0.0	4.204044343892308	0.0
15	1.0006293958900148E-4	0.0	0.0	4.293600674824464	0.0
16	1.0006293958900148E-4	0.0	0.0	4.511137505490954	0.0
17	1.0006293958900148E-4	0.0	0.0	4.702557908924714	0.0
18	1.0006293958900148E-4	0.0	0.0	4.946011040944755	0.0
19	1.0006293958900148E-4	0.0	0.0	5.077393680625113	0.0
20	1.0006293958900148E-4	0.0	0.0	5.2896271754933855	0.0
21	1.0006293958900148E-4	0.0	0.0	5.468039396780575	0.0
22	1.0006293958900148E-4	0.0	0.0	5.639347149356945	0.0
23	2.0012587917800297E-4	0.0	0.0	5.834870133313855	0.0
24	2.0012587917800297E-4	0.0	0.0	5.976959507530236	0.0
25	2.0012587917800297E-4	0.0	0.0	6.102238307895666	0.0
26	2.0012587917800297E-4	0.0	0.0	6.222213772462879	0.0
27	2.0012587917800297E-4	0.0	0.0	6.352895971566115	0.0
28	2.0012587917800297E-4	0.0	0.0	6.487080373554966	0.0
29	2.0012587917800297E-4	0.0	0.0	6.640676985824084	0.0
30	2.0012587917800297E-4	0.0	0.0	6.842303809095921	0.0
31	2.0012587917800297E-4	0.0	0.0	6.9976014913380515	0.0
32	2.0012587917800297E-4	0.0	0.0	7.1521987330030585	0.0
33	2.0012587917800297E-4	0.0	0.0	7.306295659970122	0.0
34	2.0012587917800297E-4	0.0	0.0	7.470899195594028	0.0
35	3.0018881876700445E-4	0.0	0.0	7.664921235457102	0.0
36	3.0018881876700445E-4	0.0	0.0	7.837930058006486	0.0
37	3.0018881876700445E-4	0.0	0.0	7.997730572530122	0.0
38	3.0018881876700445E-4	0.0	0.0	8.183547451346897	0.0
39	3.0018881876700445E-4	0.0	0.0	8.404086170201056	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATAG	30	2.1652158E-6	45.000004	1
CGTATCC	20	7.032846E-4	45.0	23
CGTATCA	20	7.032846E-4	45.0	36
GTCGACG	25	3.8904393E-5	45.0	1
ACCGCGA	25	3.8904393E-5	45.0	32
ACTCGTC	20	7.032846E-4	45.0	14
CGTTTTT	3550	0.0	42.46479	1
CGTAAGG	135	0.0	41.666664	2
GACGGGT	170	0.0	41.02941	4
CGATTGA	965	0.0	40.569946	20
TTCGATA	50	1.0804797E-9	40.5	15
TCAACGG	50	1.0804797E-9	40.5	2
ATTAGCG	95	0.0	40.263157	1
ATAGGGC	455	0.0	40.054943	4
TAACGGG	265	0.0	39.905663	3
ATTGAAT	1005	0.0	39.850746	22
CGTTAGG	85	0.0	39.705883	2
TTAACGG	85	0.0	39.705883	2
ATATCGG	40	3.457626E-7	39.375	2
GCGATAA	80	0.0	39.375	9
>>END_MODULE