FastQCFastQC Report
Thu 26 May 2016
SRR1547069_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547069_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1049307
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48850.4655453551725091No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC17730.16896866217417783No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAT207.033004E-445.00000421
TCGATCT207.033004E-445.00000429
TTGTACG453.8562575E-1045.01
CACGACC700.045.027
ATATGCG600.044.9999961
TAGTACG302.1653213E-644.9999961
CGTTTTT32100.042.336451
CGTTAGG2450.042.24492
ATTAGCG700.041.7857131
GACCGAT3250.040.846159
TCGTTAG850.039.7058831
CGTAGCA403.4578443E-739.37500430
ACATCGT403.4578443E-739.37500426
TACAACG403.4578443E-739.3750041
GCGTTAG1200.039.3749961
TTTAGCG750.039.0000041
TACGTAG1100.038.8636361
TATCTAG356.24777E-638.5714261
CTCTATA356.24777E-638.57142632
TATCGCG356.24777E-638.5714261
Produced by FastQC (version 0.11.5)