##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547069_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1049307
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.65888057546552	31.0	31.0	33.0	30.0	34.0
2	32.04394138226468	33.0	31.0	34.0	30.0	34.0
3	32.13941677697757	33.0	31.0	34.0	30.0	34.0
4	35.80775978812683	37.0	35.0	37.0	35.0	37.0
5	35.737083618045055	37.0	35.0	37.0	33.0	37.0
6	35.79038927596976	37.0	35.0	37.0	35.0	37.0
7	36.11194340645778	37.0	35.0	37.0	35.0	37.0
8	36.05866443281137	37.0	35.0	37.0	35.0	37.0
9	37.853554774722745	39.0	38.0	39.0	35.0	39.0
10	37.41070153920635	39.0	37.0	39.0	35.0	39.0
11	37.079709751292995	39.0	37.0	39.0	33.0	39.0
12	36.17059926217971	38.0	35.0	39.0	32.0	39.0
13	35.73503845871608	38.0	35.0	39.0	31.0	39.0
14	36.70205001967965	39.0	35.0	41.0	31.0	41.0
15	37.00603445893337	39.0	35.0	41.0	32.0	41.0
16	37.17793743870955	39.0	35.0	41.0	33.0	41.0
17	37.166903489636496	38.0	35.0	41.0	33.0	41.0
18	37.09605768378558	38.0	35.0	41.0	32.0	41.0
19	37.0467098761373	38.0	35.0	41.0	32.0	41.0
20	36.86428566663522	38.0	35.0	41.0	32.0	41.0
21	36.766550685357096	38.0	35.0	41.0	32.0	41.0
22	36.67607954583358	38.0	35.0	41.0	32.0	41.0
23	36.65927035653055	38.0	35.0	41.0	32.0	41.0
24	36.55659687774884	38.0	35.0	41.0	32.0	41.0
25	36.44698834564146	38.0	35.0	40.0	31.0	41.0
26	36.31033625049675	37.0	35.0	40.0	31.0	41.0
27	36.24962761136636	37.0	35.0	40.0	31.0	41.0
28	36.28949106410231	37.0	35.0	40.0	31.0	41.0
29	36.33023986307153	37.0	35.0	40.0	31.0	41.0
30	36.300910029190696	37.0	35.0	40.0	31.0	41.0
31	36.15085861430449	37.0	35.0	40.0	31.0	41.0
32	36.01693879865473	37.0	35.0	40.0	30.0	41.0
33	35.92763986135611	37.0	35.0	40.0	30.0	41.0
34	35.80340834474563	37.0	35.0	40.0	30.0	41.0
35	35.72792042748214	37.0	35.0	40.0	30.0	41.0
36	35.60184388362986	37.0	35.0	40.0	30.0	41.0
37	35.51022817916968	37.0	35.0	40.0	29.0	41.0
38	35.452367133736836	37.0	34.0	40.0	29.0	41.0
39	35.4202840541424	36.0	35.0	40.0	29.0	41.0
40	35.26809122592339	36.0	34.0	40.0	29.0	41.0
41	35.235955730782315	36.0	34.0	40.0	29.0	41.0
42	35.15588860076222	36.0	34.0	40.0	28.0	41.0
43	35.02362892842609	36.0	34.0	40.0	28.0	41.0
44	34.89989488300373	35.0	34.0	40.0	27.0	41.0
45	34.794612063009204	35.0	34.0	40.0	27.0	41.0
46	34.768203204591224	35.0	34.0	40.0	27.0	41.0
47	34.72689594179778	35.0	34.0	40.0	27.0	41.0
48	34.66272882959896	35.0	34.0	40.0	27.0	41.0
49	34.61402144462964	35.0	34.0	40.0	27.0	41.0
50	34.48450262887792	35.0	34.0	40.0	27.0	41.0
51	34.112099700087775	35.0	34.0	39.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	3.0
13	4.0
14	15.0
15	27.0
16	76.0
17	161.0
18	378.0
19	754.0
20	1359.0
21	2187.0
22	3245.0
23	4793.0
24	6917.0
25	10118.0
26	13729.0
27	16666.0
28	18197.0
29	20511.0
30	23639.0
31	29073.0
32	36762.0
33	48579.0
34	95069.0
35	178520.0
36	66142.0
37	86341.0
38	129221.0
39	256605.0
40	213.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.08069039852017	22.936471404460278	30.013523211033565	13.969314985985989
2	30.85512628811206	25.315184212056145	30.740574493451394	13.089115006380403
3	29.2582628344231	24.951325017368607	31.9416529194983	13.848759228709998
4	26.473091287869043	26.801498512827994	31.257772987314485	15.467637211988484
5	23.95886046695581	32.120056380067986	29.07652383906712	14.844559313909084
6	22.29490511356543	39.84648915903544	26.550570995904916	11.308034731494216
7	87.13465172728286	4.288068220263469	6.721865002330109	1.8554150501235576
8	88.57979599869246	2.8517869412860106	6.282241517496787	2.286175542524733
9	84.92328746496497	3.975480960290935	7.824306899696657	3.276924675047436
10	53.615290853868316	21.10430979684687	13.06090591218776	12.219493437097055
11	46.88780309289846	19.271481082276207	19.507922848127382	14.332792976697952
12	41.040229408552506	19.984809021573284	23.11563727298112	15.859324296893092
13	23.070845805850908	35.68145452188921	24.655510732321428	16.592188939938453
14	16.685583913954638	38.11182046817566	28.546364410034432	16.65623120783527
15	16.573700547123003	21.70699328223294	46.44484407327884	15.274462097365213
16	18.80898535890831	18.54061776010262	43.219000730958626	19.43139615003045
17	20.133192669066347	18.201250920845855	27.333373359750766	34.332183050337036
18	24.51122502756581	22.16062601316869	31.77154064539739	21.556608313868107
19	28.993135469409808	24.049015207179593	24.80418028279617	22.153669040614425
20	31.14093396880036	23.839448321606547	23.676960127017168	21.342657582575928
21	25.246281593470737	26.665980499510628	26.736789137973922	21.350948769044713
22	24.389811561344775	22.61883319181136	26.8864116983876	26.104943548456266
23	22.4735944771168	27.824364080292995	25.018702820051708	24.683338622538496
24	23.236193030257112	22.60968429639753	34.22563653916347	19.928486134181895
25	19.869399517967572	23.748245270449925	33.10241902512801	23.27993618645449
26	18.662412430299234	31.29913361866451	26.8495302137506	23.188923737285656
27	19.877118898472993	28.955015071852188	29.211946551390582	21.95591947828424
28	17.54291165502565	26.95245528715619	36.32006648197334	19.18456657584482
29	18.77315218520414	23.568031090996246	35.53764532210306	22.12117140169655
30	21.164063520018452	26.055482332625246	30.427701330497175	22.352752816859127
31	29.24168046148553	24.337777218678614	24.442417709974297	21.978124609861556
32	29.762500393116596	25.82752235523064	25.12267620439014	19.287301047262623
33	27.40380079423848	25.709253821808108	25.80712794253731	21.079817441416097
34	19.989478770274093	27.080253919968133	29.126461559867607	23.803805749890167
35	21.054657979028065	25.898998100651195	30.134460172285138	22.911883748035606
36	29.13170311453178	23.672766883285824	26.142396839056637	21.053133163125757
37	22.306055329851034	29.05336569755086	27.735734155971514	20.904844816626593
38	22.751015670342426	29.20546608380579	23.995837252586707	24.04768099326508
39	22.146807369054052	27.808734717294364	26.233313987231572	23.81114392642001
40	24.158992554133345	24.68819897322709	25.263054568396093	25.889753904243467
41	19.114425044338788	23.450239062543183	26.928439436694884	30.506896456423142
42	24.13049755695902	25.222075141021644	24.044154856490998	26.603272445528336
43	23.579276608275748	25.44298284486809	25.546289122249256	25.431451424606905
44	21.820306163972983	29.01686541688943	26.266574034100604	22.896254385036983
45	20.261944311817228	32.44512807024064	23.20398129432092	24.08894632362121
46	23.534866345121113	28.479844316296376	26.38074462478569	21.60454471379682
47	22.833736933042477	25.81294130316485	27.094644370046133	24.258677393746538
48	24.633877406707473	22.525533518789068	28.78004244706268	24.060546627440775
49	22.519529556173744	21.244974063834512	30.731044394061986	25.50445198592976
50	20.250603493543835	29.014768795023766	27.09759870085685	23.637029010575553
51	19.89389187339835	31.47258142755171	23.991167503885897	24.642359195164047
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	621.0
1	965.5
2	1310.0
3	4776.5
4	8243.0
5	5773.0
6	3303.0
7	3464.0
8	3625.0
9	3873.5
10	4122.0
11	4177.5
12	4233.0
13	4073.5
14	3914.0
15	3836.0
16	3758.0
17	3488.0
18	3218.0
19	3111.5
20	3005.0
21	3031.5
22	3058.0
23	3166.5
24	3275.0
25	3337.0
26	4202.5
27	5006.0
28	5633.0
29	6260.0
30	8441.5
31	10623.0
32	11741.5
33	12860.0
34	15692.0
35	18524.0
36	19841.5
37	21159.0
38	27566.0
39	33973.0
40	47191.5
41	60410.0
42	74288.0
43	88166.0
44	90300.0
45	92434.0
46	91598.5
47	90763.0
48	88039.5
49	85316.0
50	83854.5
51	82393.0
52	76118.0
53	69843.0
54	66131.5
55	62420.0
56	57619.5
57	52819.0
58	51185.5
59	49552.0
60	46830.0
61	44108.0
62	40277.0
63	36446.0
64	31703.5
65	26961.0
66	22443.5
67	17926.0
68	14881.0
69	11836.0
70	10227.5
71	8619.0
72	7089.0
73	5559.0
74	4422.0
75	2373.0
76	1461.0
77	1159.5
78	858.0
79	585.0
80	312.0
81	245.5
82	179.0
83	141.0
84	103.0
85	63.5
86	24.0
87	15.5
88	7.0
89	9.0
90	11.0
91	8.0
92	5.0
93	3.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1049307.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.842890505579454
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.12663382220477	17.501972891590782
2	6.581387891161385	2.875130701627692
3	2.0287990274783847	1.3294450504510933
4	1.0090115042817533	0.8815891122758543
5	0.6848565998058107	0.7479623860790885
6	0.49704509954018106	0.651414101135461
7	0.39593750114777027	0.6053893639236441
8	0.3051512848300161	0.5332308881743144
9	0.26610037560355637	0.5231161231021842
>10	5.4107396178732365	32.30082755968209
>50	2.54070123068609	36.75380684209137
>100	0.1514412447386731	4.495599664367353
>500	0.0013168803890319398	0.1621357526061636
>1k	8.779202593546266E-4	0.6383795628928667
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4885	0.4655453551725091	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1773	0.16896866217417783	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9060198778813066E-4	0.0	0.0	0.0637563649151297	0.0
2	1.9060198778813066E-4	0.0	0.0	0.24196922349703184	0.0
3	1.9060198778813066E-4	0.0	0.0	0.34699091876829186	0.0
4	1.9060198778813066E-4	0.0	0.0	0.5232977574723127	0.0
5	1.9060198778813066E-4	0.0	0.0	0.936713468984768	0.0
6	1.9060198778813066E-4	0.0	0.0	1.3388836632177237	0.0
7	1.9060198778813066E-4	0.0	0.0	1.5982929685973695	0.0
8	1.9060198778813066E-4	0.0	0.0	2.2064086106354	0.0
9	1.9060198778813066E-4	0.0	0.0	2.4475201251873857	0.0
10	1.9060198778813066E-4	0.0	0.0	2.8267227798918717	0.0
11	1.9060198778813066E-4	0.0	0.0	3.2118340962177894	0.0
12	1.9060198778813066E-4	0.0	0.0	3.5372869903660225	0.0
13	1.9060198778813066E-4	0.0	0.0	3.6863377448163406	0.0
14	1.9060198778813066E-4	0.0	0.0	3.7400875053725935	0.0
15	1.9060198778813066E-4	0.0	0.0	3.8287174296940742	0.0
16	1.9060198778813066E-4	0.0	0.0	4.013410755860773	0.0
17	1.9060198778813066E-4	0.0	0.0	4.211922726142111	0.0
18	1.9060198778813066E-4	0.0	0.0	4.506593399262561	0.0
19	1.9060198778813066E-4	0.0	0.0	4.648782482152506	0.0
20	1.9060198778813066E-4	0.0	0.0	4.8046949081631976	0.0
21	1.9060198778813066E-4	0.0	0.0	4.999490139682667	0.0
22	1.9060198778813066E-4	0.0	0.0	5.1995269258663095	0.0
23	1.9060198778813066E-4	0.0	0.0	5.418338007847084	0.0
24	1.9060198778813066E-4	0.0	0.0	5.574822239821139	0.0
25	1.9060198778813066E-4	0.0	0.0	5.711579166059122	0.0
26	1.9060198778813066E-4	0.0	0.0	5.858342696655983	0.0
27	1.9060198778813066E-4	0.0	0.0	6.0027237024054925	0.0
28	1.9060198778813066E-4	0.0	0.0	6.148820126045094	0.0
29	2.8590298168219597E-4	0.0	0.0	6.295107151672485	0.0
30	2.8590298168219597E-4	0.0	0.0	6.499718385563043	0.0
31	2.8590298168219597E-4	0.0	0.0	6.674595709358653	0.0
32	2.8590298168219597E-4	0.0	0.0	6.83346246618006	0.0
33	2.8590298168219597E-4	0.0	0.0	6.9990955935679455	0.0
34	2.8590298168219597E-4	0.0	0.0	7.166539439839818	0.0
35	3.812039755762613E-4	0.0	0.0	7.384683414863334	0.0
36	3.812039755762613E-4	0.0	0.0	7.555558096915393	0.0
37	3.812039755762613E-4	0.0	0.0	7.75054393042265	0.0
38	3.812039755762613E-4	0.0	0.0	7.9489605997100945	0.0
39	3.812039755762613E-4	0.0	0.0	8.2170422955341	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACGTAT	20	7.033004E-4	45.000004	21
TCGATCT	20	7.033004E-4	45.000004	29
TTGTACG	45	3.8562575E-10	45.0	1
CACGACC	70	0.0	45.0	27
ATATGCG	60	0.0	44.999996	1
TAGTACG	30	2.1653213E-6	44.999996	1
CGTTTTT	3210	0.0	42.33645	1
CGTTAGG	245	0.0	42.2449	2
ATTAGCG	70	0.0	41.785713	1
GACCGAT	325	0.0	40.84615	9
TCGTTAG	85	0.0	39.705883	1
CGTAGCA	40	3.4578443E-7	39.375004	30
ACATCGT	40	3.4578443E-7	39.375004	26
TACAACG	40	3.4578443E-7	39.375004	1
GCGTTAG	120	0.0	39.374996	1
TTTAGCG	75	0.0	39.000004	1
TACGTAG	110	0.0	38.863636	1
TATCTAG	35	6.24777E-6	38.571426	1
CTCTATA	35	6.24777E-6	38.571426	32
TATCGCG	35	6.24777E-6	38.571426	1
>>END_MODULE