FastQCFastQC Report
Thu 26 May 2016
SRR1547068_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547068_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences970691
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86740.8935902362337758No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG453.8380676E-1045.01
TCGATAG406.8139343E-945.01
ATCGCGC253.8903563E-545.010
CGTTTTT53350.043.35521
TAACGAG501.0804797E-940.51
CACGCGG2400.039.37499637
CGGGATC1200.039.3749966
TTACACG2400.039.37499634
TGCGGGC2750.039.2727284
GACCGAT1950.039.230779
GTACGGG4250.039.176473
TACGGGT750.039.04
AACGGGA1800.038.754
AGGGCGA7500.038.46
TAGGGAG9150.038.3606575
GGGCGAT12750.038.1176457
TGCGTTG659.094947E-1238.076921
TATCGGG950.037.8947373
CGGGATT2450.037.653066
GTTTTTT64850.037.609872
Produced by FastQC (version 0.11.5)