##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547068_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	970691
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.66456781818313	31.0	31.0	33.0	30.0	34.0
2	32.05702638635776	33.0	31.0	34.0	30.0	34.0
3	32.11385394528228	33.0	31.0	34.0	30.0	34.0
4	35.81431681142609	37.0	35.0	37.0	35.0	37.0
5	35.76065812910597	37.0	35.0	37.0	35.0	37.0
6	35.8216620943225	37.0	35.0	37.0	35.0	37.0
7	36.116531419370325	37.0	35.0	37.0	35.0	37.0
8	36.066077670443015	37.0	35.0	37.0	35.0	37.0
9	37.86339525142399	39.0	38.0	39.0	35.0	39.0
10	37.417192494831	39.0	37.0	39.0	35.0	39.0
11	37.14369969434146	39.0	37.0	39.0	33.0	39.0
12	36.522727623929754	39.0	35.0	39.0	33.0	39.0
13	36.26771959356788	39.0	35.0	39.0	32.0	39.0
14	37.313297434507994	40.0	35.0	41.0	32.0	41.0
15	37.50237511216237	40.0	35.0	41.0	33.0	41.0
16	37.61599108264113	40.0	35.0	41.0	33.0	41.0
17	37.597732955183474	40.0	35.0	41.0	33.0	41.0
18	37.48618767455349	39.0	35.0	41.0	33.0	41.0
19	37.40979158146104	39.0	35.0	41.0	33.0	41.0
20	37.2070051128526	39.0	35.0	41.0	33.0	41.0
21	37.12627705418099	39.0	35.0	41.0	32.0	41.0
22	37.062756325133336	39.0	35.0	41.0	32.0	41.0
23	37.02332771190832	38.0	35.0	41.0	32.0	41.0
24	36.95876030580278	38.0	35.0	41.0	32.0	41.0
25	36.84897047567146	38.0	35.0	41.0	32.0	41.0
26	36.71064427299728	38.0	35.0	41.0	32.0	41.0
27	36.616559749704074	38.0	35.0	41.0	31.0	41.0
28	36.64213431462741	38.0	35.0	41.0	31.0	41.0
29	36.65065710921395	38.0	35.0	41.0	31.0	41.0
30	36.584244625735685	38.0	35.0	41.0	31.0	41.0
31	36.43005343616043	38.0	35.0	41.0	31.0	41.0
32	36.28470852207345	38.0	35.0	41.0	31.0	41.0
33	36.10903984893236	38.0	35.0	41.0	30.0	41.0
34	35.91194417172921	38.0	35.0	41.0	30.0	41.0
35	35.80438162092777	38.0	35.0	41.0	29.0	41.0
36	35.69968403951412	38.0	35.0	41.0	29.0	41.0
37	35.63296455823738	38.0	35.0	41.0	29.0	41.0
38	35.56254874105148	38.0	35.0	41.0	28.0	41.0
39	35.49783916818019	38.0	35.0	41.0	28.0	41.0
40	35.38336298574933	38.0	35.0	40.0	27.0	41.0
41	35.342231461917336	38.0	35.0	40.0	27.0	41.0
42	35.26072148603418	38.0	35.0	40.0	27.0	41.0
43	35.14702310003904	37.0	34.0	40.0	27.0	41.0
44	34.94769396234229	37.0	34.0	40.0	26.0	41.0
45	34.88251153044584	37.0	34.0	40.0	26.0	41.0
46	34.86203333501599	37.0	34.0	40.0	26.0	41.0
47	34.837496175404944	37.0	34.0	40.0	26.0	41.0
48	34.753091354509316	36.0	34.0	40.0	26.0	41.0
49	34.693886107937544	36.0	34.0	40.0	26.0	41.0
50	34.59469594340526	36.0	34.0	40.0	25.0	41.0
51	34.23057904111607	35.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	4.0
12	1.0
13	3.0
14	9.0
15	21.0
16	54.0
17	134.0
18	320.0
19	668.0
20	1145.0
21	1914.0
22	2892.0
23	4476.0
24	6727.0
25	10659.0
26	14688.0
27	17196.0
28	17891.0
29	18491.0
30	20763.0
31	25160.0
32	31000.0
33	40758.0
34	74957.0
35	130102.0
36	62394.0
37	83036.0
38	129039.0
39	275927.0
40	259.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.31935703534904	22.920167179874955	28.21217050534104	15.54830527943496
2	32.70937919482101	24.00568254985366	29.793930303258193	13.491007952067136
3	28.765384658969744	24.245923780070076	33.47738878798712	13.51130277297307
4	26.105732926338042	26.072766719790337	32.99711236634521	14.82438798752641
5	23.436706428719333	31.093829035192456	31.264223115285915	14.205241420802295
6	21.458940074647852	39.543582870347	28.116774545143613	10.880702509861532
7	85.93939781042576	3.724872281704476	8.549270571170434	1.7864593366993204
8	87.2742201174215	2.7122946437125717	8.097427502675929	1.9160577361899924
9	83.23400546620913	4.093475678666023	9.81888160083899	2.8536372542858643
10	46.86784981008375	24.275696385358472	16.00447516253885	12.851978642018933
11	39.146340081447136	22.173276562778472	23.873920743058296	14.806462612716095
12	36.067399409286786	20.538976873175912	26.718595309939	16.6750284075983
13	23.931611604516785	29.88520548763716	27.869734034826738	18.313448873019322
14	17.889421041299443	32.74368465350971	30.76797868734747	18.59891561784337
15	17.704501226445903	23.648720344579274	41.26586112367375	17.380917305301068
16	20.697214664604903	21.164201584232263	38.99067777490468	19.14790597625815
17	21.110940556778623	21.06262446030714	29.297582855924286	28.52885212698995
18	23.56187499420516	23.785427082356794	32.16440659282923	20.488291330608813
19	27.52400094365766	24.938523175758302	27.062268013198842	20.475207867385194
20	29.47673358463198	24.344925419108655	26.615987992059264	19.5623530042001
21	24.781933694656693	25.935544885035505	29.39153654458525	19.890984875722552
22	23.60071330629418	23.508716986146982	28.94608067860936	23.94448902894948
23	21.915625054729055	27.412121880186387	28.27841197662284	22.393841088461723
24	22.155557226759083	23.576709787151625	33.85443977537651	20.41329321071278
25	20.70267469256437	25.224401998164193	32.228690695597265	21.844232613674176
26	19.82639171476814	29.700079633992694	28.246063886447903	22.227464764791264
27	20.15172696563582	28.14448676252278	30.4552118027261	21.2485744691153
28	18.594485783838525	26.87971764444092	34.6876606458698	19.83813592585076
29	19.68381287144931	25.407982560876736	33.979505321466874	20.92869924620708
30	22.183166424742787	26.061949683266867	31.448215755580307	20.306668136410043
31	27.63505585196525	25.06595816794428	26.7003608769423	20.59862510314817
32	27.96574811139693	26.1200526223072	27.108008624783785	18.806190641512078
33	26.609394750749725	25.759278699400735	27.07318806911777	20.558138480731767
34	21.437306001600923	26.49658851271929	29.974935381084194	22.09117010459559
35	21.982587661779082	26.544492531608928	29.69729811031523	21.775621696296763
36	27.08482926080493	26.1302515424579	26.56612660465586	20.21879259208131
37	22.699293596005322	28.76445748441059	27.880138993768355	20.656109925815734
38	22.096733151950517	29.921674353630557	25.63029841628283	22.351294078136092
39	22.707329108851322	27.6448426945341	27.1010033058924	22.546824890722174
40	24.027728700482438	25.133847949553463	27.24327309102485	23.595150258939253
41	19.905304571691712	24.619884185595623	27.95719750157362	27.517613741139048
42	22.630785698023367	26.26015900013495	25.86817020040363	25.24088510143805
43	22.750803293736112	26.012912451027155	27.30941154291118	23.92687271232555
44	22.200576702575795	28.296852448410466	27.49021058194626	22.01236026706748
45	20.761910844954777	30.157176691655742	25.64564830620661	23.43526415718287
46	22.551975860495254	29.218154902023404	26.567259818005933	21.662609419475405
47	22.13258390157115	26.29312520668266	28.208152748918035	23.36613814282815
48	23.051001812111167	24.37840672263367	29.870679752877074	22.69991171237809
49	22.293706236073067	24.085316542545463	29.948150338264185	23.672826883117285
50	20.09856895757764	28.245033692493287	28.479505836563852	23.176891513365224
51	20.052828345992697	28.512162984925173	27.14931940236388	24.285689266718244
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	412.0
1	784.0
2	1156.0
3	7722.0
4	14288.0
5	9798.0
6	5308.0
7	5235.5
8	5163.0
9	5277.5
10	5392.0
11	5433.5
12	5475.0
13	5224.0
14	4973.0
15	4688.0
16	4403.0
17	4194.0
18	3985.0
19	3938.0
20	3891.0
21	3844.5
22	3798.0
23	3924.0
24	4050.0
25	4538.5
26	5738.5
27	6450.0
28	7602.0
29	8754.0
30	10650.0
31	12546.0
32	14648.0
33	16750.0
34	18995.5
35	21241.0
36	22816.0
37	24391.0
38	30722.5
39	37054.0
40	44592.5
41	52131.0
42	61481.5
43	70832.0
44	73361.5
45	75891.0
46	76152.0
47	76413.0
48	76847.5
49	77282.0
50	74686.5
51	72091.0
52	69068.0
53	66045.0
54	60365.0
55	54685.0
56	52423.5
57	50162.0
58	47258.0
59	44354.0
60	41882.0
61	39410.0
62	35704.5
63	31999.0
64	27287.5
65	22576.0
66	19227.5
67	15879.0
68	13055.5
69	10232.0
70	8317.5
71	6403.0
72	5469.0
73	4535.0
74	3658.0
75	2079.5
76	1378.0
77	986.5
78	595.0
79	445.0
80	295.0
81	211.0
82	127.0
83	92.5
84	58.0
85	38.5
86	19.0
87	14.0
88	9.0
89	5.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	970691.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.298779044338882
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.92866835873626	20.231300513416286
2	6.363000100314036	3.3467826739452993
3	2.414087865152859	1.904626900778244
4	1.4374325709538438	1.5121088629860444
5	1.0326575144514862	1.3578815900517913
6	0.8169563843810244	1.2890973265019126
7	0.658946650068332	1.2130644646508049
8	0.5720971774420706	1.203636580915116
9	0.5077734995306642	1.2018440761854885
>10	8.68612391540034	55.57431675471396
>50	0.5502812297396673	8.802628832063618
>100	0.031185234228681205	1.3870639014577633
>500	3.947498003630531E-4	0.07516160950942448
>1k	0.0	0.0
>5k	3.947498003630531E-4	0.9004859128242374
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8674	0.8935902362337758	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0301939546158355E-4	0.0	0.0	0.05604255113110145	0.0
2	1.0301939546158355E-4	0.0	0.0	0.15257172467860525	0.0
3	1.0301939546158355E-4	0.0	0.0	0.24776164608510845	0.0
4	1.0301939546158355E-4	0.0	0.0	0.3521202936876926	0.0
5	1.0301939546158355E-4	0.0	0.0	0.6202797800741946	0.0
6	1.0301939546158355E-4	0.0	0.0	1.0190678599059846	0.0
7	1.0301939546158355E-4	0.0	0.0	1.2807371243784067	0.0
8	1.0301939546158355E-4	0.0	0.0	1.8530098661675034	0.0
9	1.0301939546158355E-4	0.0	0.0	2.156401985801867	0.0
10	1.0301939546158355E-4	0.0	0.0	2.612777907696682	0.0
11	1.0301939546158355E-4	0.0	0.0	2.9980704467230046	0.0
12	1.0301939546158355E-4	0.0	0.0	3.316812456281144	0.0
13	1.0301939546158355E-4	0.0	0.0	3.4727838210099815	0.0
14	1.0301939546158355E-4	0.0	0.0	3.550357425792554	0.0
15	1.0301939546158355E-4	0.0	0.0	3.637202776166669	0.0
16	1.0301939546158355E-4	0.0	0.0	3.794822451222892	0.0
17	1.0301939546158355E-4	0.0	0.0	3.978299994539972	0.0
18	1.0301939546158355E-4	0.0	0.0	4.230388455234467	0.0
19	1.0301939546158355E-4	0.0	0.0	4.361429126261601	0.0
20	1.0301939546158355E-4	0.0	0.0	4.505244202325972	0.0
21	1.0301939546158355E-4	0.0	0.0	4.675947340605816	0.0
22	1.0301939546158355E-4	0.0	0.0	4.85839468996828	0.0
23	1.0301939546158355E-4	0.0	0.0	5.0684512373144495	0.0
24	1.0301939546158355E-4	0.0	0.0	5.242554015644526	0.0
25	1.0301939546158355E-4	0.0	0.0	5.381836238308586	0.0
26	1.0301939546158355E-4	0.0	0.0	5.522972810090955	0.0
27	1.0301939546158355E-4	0.0	0.0	5.672247914114791	0.0
28	1.0301939546158355E-4	0.0	0.0	5.813693544083544	0.0
29	1.0301939546158355E-4	0.0	0.0	5.980791003522233	0.0
30	1.0301939546158355E-4	0.0	0.0	6.186726775049939	0.0
31	1.0301939546158355E-4	0.0	0.0	6.354133292675012	0.0
32	1.0301939546158355E-4	0.0	0.0	6.51731601508616	0.0
33	1.0301939546158355E-4	0.0	0.0	6.688431230947851	0.0
34	1.0301939546158355E-4	0.0	0.0	6.861297776532388	0.0
35	1.0301939546158355E-4	0.0	0.0	7.05919803521409	0.0
36	1.0301939546158355E-4	0.0	0.0	7.233197794148705	0.0
37	1.0301939546158355E-4	0.0	0.0	7.426874257616482	0.0
38	1.0301939546158355E-4	0.0	0.0	7.662582634432584	0.0
39	1.0301939546158355E-4	0.0	0.0	7.967932122580718	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	45	3.8380676E-10	45.0	1
TCGATAG	40	6.8139343E-9	45.0	1
ATCGCGC	25	3.8903563E-5	45.0	10
CGTTTTT	5335	0.0	43.3552	1
TAACGAG	50	1.0804797E-9	40.5	1
CACGCGG	240	0.0	39.374996	37
CGGGATC	120	0.0	39.374996	6
TTACACG	240	0.0	39.374996	34
TGCGGGC	275	0.0	39.272728	4
GACCGAT	195	0.0	39.23077	9
GTACGGG	425	0.0	39.17647	3
TACGGGT	75	0.0	39.0	4
AACGGGA	180	0.0	38.75	4
AGGGCGA	750	0.0	38.4	6
TAGGGAG	915	0.0	38.360657	5
GGGCGAT	1275	0.0	38.117645	7
TGCGTTG	65	9.094947E-12	38.07692	1
TATCGGG	95	0.0	37.894737	3
CGGGATT	245	0.0	37.65306	6
GTTTTTT	6485	0.0	37.60987	2
>>END_MODULE