##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547067_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	986441
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.657179699546145	31.0	31.0	33.0	30.0	34.0
2	32.03819285694735	33.0	31.0	34.0	30.0	34.0
3	32.11934013286147	33.0	31.0	34.0	30.0	34.0
4	35.7929668373476	37.0	35.0	37.0	35.0	37.0
5	35.733332252004935	37.0	35.0	37.0	33.0	37.0
6	35.79055006837712	37.0	35.0	37.0	35.0	37.0
7	36.12076140387514	37.0	35.0	37.0	35.0	37.0
8	36.06407681756942	37.0	35.0	37.0	35.0	37.0
9	37.83850630701684	39.0	38.0	39.0	35.0	39.0
10	37.39232250078819	39.0	37.0	39.0	35.0	39.0
11	37.1293194423184	39.0	37.0	39.0	33.0	39.0
12	36.54279880905194	39.0	35.0	39.0	33.0	39.0
13	36.30932919454889	39.0	35.0	39.0	32.0	39.0
14	37.359505535556615	40.0	35.0	41.0	32.0	41.0
15	37.54214190204989	40.0	35.0	41.0	33.0	41.0
16	37.65606457963528	40.0	35.0	41.0	33.0	41.0
17	37.61181358033577	40.0	35.0	41.0	33.0	41.0
18	37.516057219843866	39.0	35.0	41.0	33.0	41.0
19	37.45583060720307	39.0	35.0	41.0	33.0	41.0
20	37.26648223259171	39.0	35.0	41.0	32.0	41.0
21	37.20696321422163	39.0	35.0	41.0	32.0	41.0
22	37.13936160398848	39.0	35.0	41.0	32.0	41.0
23	37.089778304024264	39.0	35.0	41.0	32.0	41.0
24	37.00867360541584	39.0	35.0	41.0	32.0	41.0
25	36.91855569669144	38.0	35.0	41.0	32.0	41.0
26	36.76829227495613	38.0	35.0	41.0	32.0	41.0
27	36.6692138708752	38.0	35.0	41.0	31.0	41.0
28	36.71295292876107	38.0	35.0	41.0	31.0	41.0
29	36.727113937883765	38.0	35.0	41.0	31.0	41.0
30	36.66160976682843	38.0	35.0	41.0	31.0	41.0
31	36.5292825419868	38.0	35.0	41.0	31.0	41.0
32	36.41163232266299	38.0	35.0	41.0	31.0	41.0
33	36.26509036019387	38.0	35.0	41.0	30.0	41.0
34	36.10722486190254	38.0	35.0	41.0	30.0	41.0
35	36.0023812878824	38.0	35.0	41.0	30.0	41.0
36	35.917445645507435	38.0	35.0	41.0	30.0	41.0
37	35.833454813820595	38.0	35.0	41.0	29.0	41.0
38	35.770792171047226	38.0	35.0	41.0	29.0	41.0
39	35.707466538799586	38.0	35.0	41.0	29.0	41.0
40	35.60020315457285	38.0	35.0	41.0	28.0	41.0
41	35.559247841482666	38.0	35.0	40.0	28.0	41.0
42	35.4816892241908	38.0	35.0	40.0	28.0	41.0
43	35.352002806047196	38.0	35.0	40.0	27.0	41.0
44	35.20912451935798	37.0	34.0	40.0	27.0	41.0
45	35.122225252194504	37.0	34.0	40.0	27.0	41.0
46	35.08166225856387	37.0	34.0	40.0	27.0	41.0
47	35.021846212799346	37.0	34.0	40.0	27.0	41.0
48	34.95418681907991	37.0	34.0	40.0	27.0	41.0
49	34.88384505510213	36.0	34.0	40.0	26.0	41.0
50	34.78481024207226	36.0	34.0	40.0	26.0	41.0
51	34.40344126004495	36.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	4.0
13	4.0
14	9.0
15	30.0
16	67.0
17	173.0
18	345.0
19	729.0
20	1205.0
21	1927.0
22	3064.0
23	4534.0
24	6570.0
25	9751.0
26	13124.0
27	15611.0
28	16928.0
29	18331.0
30	21433.0
31	25282.0
32	31605.0
33	41469.0
34	74680.0
35	124752.0
36	65743.0
37	88129.0
38	135279.0
39	285417.0
40	242.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.79958862212742	22.421107800669272	29.111117644136854	14.66818593306645
2	31.541065304463217	25.135411038267875	29.791543538843175	13.531980118425736
3	29.653268669895105	24.520371720153562	32.138059954928885	13.688299655022448
4	26.427024018669137	26.757302261361804	31.910575493111093	14.905098226857968
5	24.5042531687146	31.243936535484636	29.674861446351073	14.576948849449689
6	22.181052896219843	40.519199830501776	26.332137451707705	10.967609821570678
7	86.8903462041825	3.7121328087538945	7.612112635220961	1.7854083518426342
8	87.60179270731852	2.923844406305091	7.272203811479855	2.202159074896522
9	83.33868928805677	4.525562096465982	8.81857100424658	3.317177611230677
10	45.361962854342025	28.34188765471022	14.484191147772648	11.81195834317511
11	37.686592507813444	22.569013250665776	24.316203401926725	15.428190839594055
12	33.393178101883436	21.537527333109633	27.492470406238184	17.576824158768744
13	24.14650242639955	29.844765170952954	28.51179137931209	17.496941023335406
14	18.426545530852835	32.36605128943343	30.77386280578362	18.433540373930118
15	18.011214051321872	24.233887277596935	40.61864825164404	17.13625041943715
16	21.30608926433512	22.08018523155465	37.64513032203649	18.968595182073738
17	21.575948282766024	21.46950501854647	29.28984095348835	27.664705745199154
18	24.17590104223162	23.56887031256811	31.688261132698255	20.566967512502014
19	26.990565071808653	25.18295569628594	27.134212791236372	20.692266440669034
20	28.258355035932205	25.267603434974824	26.17024231555663	20.30379921353634
21	24.841019381797796	26.192037841087302	28.997578162302663	19.96936461481224
22	23.78662281880011	23.084300024025765	29.4194989867615	23.709578170412623
23	21.791571923713633	26.974547894907047	28.620262134278683	22.613618047100637
24	22.12843951133418	24.285081418959674	33.15393419373282	20.43254487597332
25	21.187683804708037	25.546890285379458	31.424079088359058	21.841346821553444
26	20.0580673349952	29.704564185795196	27.887222854686698	22.350145624522906
27	20.219151474847457	27.34861993773576	30.571316480154415	21.86091210726237
28	18.429181268823985	26.54654459820709	34.75778074917811	20.26649338379082
29	20.087364576289914	25.091414489057122	33.404836173678916	21.416384760974047
30	22.591923896107318	25.225735751048468	31.41465125638533	20.767689096458884
31	27.13755815096899	24.759919751916232	26.910377812763258	21.19214428435152
32	27.88519536393966	25.5928129507999	26.763384733602923	19.758606951657523
33	25.78329570648422	25.590481336440803	27.322262558024253	21.303960399050727
34	21.983473922920886	26.1996409313887	29.262064330253914	22.554820815436504
35	21.647417331599154	26.410905467230172	29.698278964479375	22.2433982366913
36	26.859082296863168	25.294163563761035	27.14749285562948	20.699261283746317
37	22.497544201832646	28.4678962046387	27.668456603081175	21.366102990447477
38	22.0490632485876	29.707199923766346	25.59473906700958	22.64899776063647
39	22.13877971414408	28.28298904850873	26.300508596053895	23.2777226412933
40	23.58559711123118	24.955978107154912	27.43782953060548	24.020595251008423
41	19.939661875368117	25.12841619519059	27.82862837209727	27.103293557344028
42	22.89787224983552	26.216469104589123	26.509441517536274	24.376217128039084
43	23.338547363704468	25.68739539414927	26.9176767794526	24.056380462693664
44	22.432056250703287	28.02438260372389	27.445331246369527	22.098229899203297
45	21.25530062112179	29.112334138585076	25.789783676874745	23.842581563418392
46	23.333782760448926	27.986164403142205	27.228997983660452	21.451054852748417
47	22.353795107867576	26.275469085327963	28.34472614175607	23.02600966504839
48	23.020636814568736	24.469887200552286	29.381280786179815	23.12819519869916
49	22.084240213048727	24.19060035014765	29.764071039220795	23.961088397582824
50	20.789281872914852	28.00005271475942	28.20776914179358	23.002896270532144
51	20.5764967190131	28.79878269455548	26.893042766876075	23.73167781955535
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	453.0
1	859.5
2	1266.0
3	6267.0
4	11268.0
5	7785.5
6	4303.0
7	4223.5
8	4144.0
9	4311.5
10	4479.0
11	4457.5
12	4436.0
13	4279.0
14	4122.0
15	3922.0
16	3722.0
17	3520.0
18	3318.0
19	3428.0
20	3538.0
21	3628.5
22	3719.0
23	4112.5
24	4506.0
25	5217.5
26	7205.0
27	8481.0
28	9881.5
29	11282.0
30	12493.0
31	13704.0
32	16350.0
33	18996.0
34	21455.0
35	23914.0
36	26782.0
37	29650.0
38	32764.0
39	35878.0
40	45402.5
41	54927.0
42	62766.5
43	70606.0
44	72665.0
45	74724.0
46	77414.5
47	80105.0
48	80350.0
49	80595.0
50	77045.0
51	73495.0
52	69135.0
53	64775.0
54	59568.5
55	54362.0
56	51312.5
57	48263.0
58	45722.0
59	43181.0
60	41627.5
61	40074.0
62	35934.5
63	31795.0
64	27762.5
65	23730.0
66	20065.0
67	16400.0
68	13728.0
69	11056.0
70	8950.0
71	6844.0
72	5585.5
73	4327.0
74	3588.5
75	2233.5
76	1617.0
77	1250.0
78	883.0
79	627.0
80	371.0
81	288.5
82	206.0
83	138.5
84	71.0
85	56.0
86	41.0
87	31.0
88	21.0
89	14.0
90	7.0
91	5.5
92	4.0
93	3.0
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	986441.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.630181558043
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.95667591463237	19.724135757955022
2	5.817429030394204	2.98203524500067
3	2.3522356376837728	1.808646793834024
4	1.3325114553934239	1.3661004211962227
5	0.9714401195258395	1.244909331810713
6	0.770424669915759	1.184767450004175
7	0.6506270338768222	1.167298230338376
8	0.5587582329206877	1.1456859965446802
9	0.5148817155614551	1.1876860665681073
>10	9.644327358445102	59.78558817569495
>50	0.3988154680743661	6.165091814904349
>100	0.029881278352660922	1.3131990532313913
>500	0.0015936681788085818	0.2469140988113144
>1k	0.0	0.0
>5k	3.9841704470214545E-4	0.6779415641060035
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6639	0.6730255534796303	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0137453735195516E-4	0.0	0.0	0.05717523906650271	0.0
2	1.0137453735195516E-4	0.0	0.0	0.18723877048906118	0.0
3	1.0137453735195516E-4	0.0	0.0	0.28334183189871465	0.0
4	1.0137453735195516E-4	0.0	0.0	0.43854624858455804	0.0
5	1.0137453735195516E-4	0.0	0.0	0.8129224150253285	0.0
6	1.0137453735195516E-4	0.0	0.0	1.2493397983254955	0.0
7	1.0137453735195516E-4	0.0	0.0	1.5589376353983664	0.0
8	1.0137453735195516E-4	0.0	0.0	2.265416786204142	0.0
9	1.0137453735195516E-4	0.0	0.0	2.6347242257773145	0.0
10	1.0137453735195516E-4	0.0	0.0	3.187823701569582	0.0
11	1.0137453735195516E-4	0.0	0.0	3.5893682440206764	0.0
12	1.0137453735195516E-4	0.0	0.0	3.9822959507968547	0.0
13	1.0137453735195516E-4	0.0	0.0	4.150273559189044	0.0
14	1.0137453735195516E-4	0.0	0.0	4.236340541400854	0.0
15	1.0137453735195516E-4	0.0	0.0	4.328287246779078	0.0
16	1.0137453735195516E-4	0.0	0.0	4.503361072785904	0.0
17	1.0137453735195516E-4	0.0	0.0	4.6949589483811	0.0
18	1.0137453735195516E-4	0.0	0.0	4.952855771404473	0.0
19	1.0137453735195516E-4	0.0	0.0	5.105931322805926	0.0
20	1.0137453735195516E-4	0.0	0.0	5.264379724687031	0.0
21	1.0137453735195516E-4	0.0	0.0	5.437121936334763	0.0
22	1.0137453735195516E-4	0.0	0.0	5.631963797125221	0.0
23	1.0137453735195516E-4	0.0	0.0	5.835321119053243	0.0
24	1.0137453735195516E-4	0.0	0.0	5.9932626482475895	0.0
25	1.0137453735195516E-4	0.0	0.0	6.125556419491891	0.0
26	1.0137453735195516E-4	0.0	0.0	6.263425790290549	0.0
27	1.0137453735195516E-4	0.0	0.0	6.410925742137644	0.0
28	1.0137453735195516E-4	0.0	0.0	6.568867271331991	0.0
29	1.0137453735195516E-4	0.0	0.0	6.742927351965298	0.0
30	1.0137453735195516E-4	0.0	0.0	6.969398068409565	0.0
31	1.0137453735195516E-4	0.0	0.0	7.141025160146425	0.0
32	1.0137453735195516E-4	0.0	0.0	7.314274244480917	0.0
33	1.0137453735195516E-4	0.0	0.0	7.4877260778901125	0.0
34	1.0137453735195516E-4	0.0	0.0	7.666652136316313	0.0
35	1.0137453735195516E-4	0.0	0.0	7.868691589258759	0.0
36	1.0137453735195516E-4	0.0	0.0	8.056234483359876	0.0
37	1.0137453735195516E-4	0.0	0.0	8.24306775569953	0.0
38	1.0137453735195516E-4	0.0	0.0	8.471565962890837	0.0
39	1.0137453735195516E-4	0.0	0.0	8.755211918401608	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCCA	30	2.1651867E-6	45.000004	37
CGAACGT	25	3.890402E-5	45.0	38
CGTTTTT	3700	0.0	42.81081	1
GACGTAG	50	1.0804797E-9	40.5	1
CTACGGG	145	0.0	40.34483	3
CGGGTAT	90	0.0	40.0	6
TACGGGA	230	0.0	39.130436	4
TAAGGGA	1090	0.0	39.013763	4
GGGCGAT	1375	0.0	38.945457	7
TAGGGAC	955	0.0	38.638744	5
ACGTTCG	35	6.24739E-6	38.571426	41
CGGTCTA	70	0.0	38.571426	31
ACGGGAT	335	0.0	38.283585	5
GCGTAAG	65	9.094947E-12	38.07692	1
ATAGGGC	375	0.0	37.8	4
ATAGGGA	1000	0.0	37.8	4
GTAGGGA	1230	0.0	37.68293	4
ACGTTAG	30	1.1398045E-4	37.500004	1
CCGAACG	30	1.1398045E-4	37.500004	37
TCGGTAC	30	1.1398045E-4	37.500004	36
>>END_MODULE