FastQCFastQC Report
Thu 26 May 2016
SRR1547066_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547066_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences958191
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54590.5697193983245512No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC10230.10676368281480415No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACG207.032698E-445.01
CCGATTA207.032698E-445.011
CACTCGA207.032698E-445.038
CACTACG207.032698E-445.01
TCGAGTA207.032698E-445.035
TATCGCG207.032698E-445.01
TACGTAG253.8903177E-545.01
CGTTTTT30100.043.0564771
ACTACGG603.6379788E-1241.2500042
ATTAGCG556.002665E-1140.9090921
GCGTAAG451.927765E-840.01
GGGCGAT14000.039.6964267
TCGTTAG403.457426E-739.3751
ATGGTCG403.457426E-739.37544
ATACGAG403.457426E-739.3751
CTAGACG356.247201E-638.5714261
TAGGGCG3100.038.467745
ATAGGGA9650.038.2383424
TATAGCG659.094947E-1238.0769271
TCACGAC950.037.89473725