##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1547066_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 958191 Sequences flagged as poor quality 0 Sequence length 51 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.663167364335504 31.0 31.0 33.0 30.0 34.0 2 32.049872102743606 33.0 31.0 34.0 30.0 34.0 3 32.13979989375813 33.0 31.0 34.0 30.0 34.0 4 35.81794861358539 37.0 35.0 37.0 35.0 37.0 5 35.75716949960916 37.0 35.0 37.0 35.0 37.0 6 35.80278670953912 37.0 35.0 37.0 35.0 37.0 7 36.103272729549744 37.0 35.0 37.0 35.0 37.0 8 36.044168646960784 37.0 35.0 37.0 35.0 37.0 9 37.80839310742848 39.0 38.0 39.0 35.0 39.0 10 37.42093590943768 39.0 37.0 39.0 35.0 39.0 11 37.09889364437779 39.0 37.0 39.0 33.0 39.0 12 36.338529583350294 38.0 35.0 39.0 33.0 39.0 13 36.015687895210874 38.0 35.0 39.0 32.0 39.0 14 37.03239333285326 39.0 35.0 41.0 32.0 41.0 15 37.24236399632224 39.0 35.0 41.0 32.0 41.0 16 37.390202997106 39.0 35.0 41.0 33.0 41.0 17 37.35761659209907 39.0 35.0 41.0 33.0 41.0 18 37.26771071738307 39.0 35.0 41.0 33.0 41.0 19 37.206745836686004 39.0 35.0 41.0 32.0 41.0 20 37.00983102533837 38.0 35.0 41.0 32.0 41.0 21 36.92522472033237 38.0 35.0 41.0 32.0 41.0 22 36.85014887428498 38.0 35.0 41.0 32.0 41.0 23 36.8051515825133 38.0 35.0 41.0 32.0 41.0 24 36.72725583938902 38.0 35.0 41.0 32.0 41.0 25 36.633730644516596 38.0 35.0 41.0 32.0 41.0 26 36.46973828808661 38.0 35.0 41.0 31.0 41.0 27 36.372146054387905 38.0 35.0 40.0 31.0 41.0 28 36.40466149233295 38.0 35.0 40.0 31.0 41.0 29 36.413361219214124 38.0 35.0 40.0 31.0 41.0 30 36.38393180482806 38.0 35.0 40.0 31.0 41.0 31 36.235098221544554 38.0 35.0 40.0 31.0 41.0 32 36.10576805668181 38.0 35.0 41.0 30.0 41.0 33 35.99639842160905 38.0 35.0 41.0 30.0 41.0 34 35.82467587359931 38.0 35.0 41.0 30.0 41.0 35 35.719911792116605 38.0 35.0 41.0 30.0 41.0 36 35.60534590702689 38.0 35.0 40.0 29.0 41.0 37 35.51794579577558 37.0 35.0 40.0 29.0 41.0 38 35.460901845247975 37.0 35.0 40.0 29.0 41.0 39 35.392009526284426 37.0 35.0 40.0 29.0 41.0 40 35.270536876259534 37.0 34.0 40.0 28.0 41.0 41 35.24224502213024 37.0 34.0 40.0 28.0 41.0 42 35.17247187669264 37.0 34.0 40.0 28.0 41.0 43 35.05972400074724 36.0 34.0 40.0 27.0 41.0 44 34.918515202083924 36.0 34.0 40.0 27.0 41.0 45 34.81916757723669 36.0 34.0 40.0 27.0 41.0 46 34.78437284424504 36.0 34.0 40.0 27.0 41.0 47 34.7186364722691 35.0 34.0 40.0 27.0 41.0 48 34.656571602112734 35.0 34.0 40.0 26.0 41.0 49 34.595383383897364 36.0 34.0 40.0 26.0 41.0 50 34.46598955740557 35.0 34.0 40.0 26.0 41.0 51 34.1014557640387 35.0 33.0 39.0 25.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 2.0 11 3.0 12 4.0 13 11.0 14 19.0 15 20.0 16 95.0 17 193.0 18 423.0 19 747.0 20 1385.0 21 2156.0 22 3181.0 23 4702.0 24 6787.0 25 9598.0 26 12811.0 27 15306.0 28 16539.0 29 18241.0 30 21162.0 31 25772.0 32 32777.0 33 43073.0 34 81968.0 35 143775.0 36 64436.0 37 84071.0 38 125254.0 39 243505.0 40 175.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.169283577073884 22.570134764363264 29.02250177678563 14.238079881777225 2 31.978697357833667 25.01004497015731 29.522089019830076 13.489168652178948 3 29.60902367064604 25.18579281166281 31.608625002739537 13.596558514951612 4 26.71815953186786 27.40163495586997 30.770483129146488 15.109722383115685 5 25.0595131868281 30.587638581451927 29.620399273213792 14.732448958506186 6 22.95721834164587 40.227470306024586 26.033327384623732 10.781983967705813 7 86.40291966841684 4.104505260433463 7.539937235895558 1.9526378352541403 8 87.33999797535147 3.432718528978043 6.876708297197531 2.3505751984729555 9 82.77264136273456 4.795807933908793 8.716738103363527 3.7148125999931123 10 51.590236184643764 23.55501147474773 13.32218733008346 11.532565010525042 11 43.19055386660906 20.366920582639576 21.264967005534388 15.177558545216977 12 37.588330510305354 20.388419427859372 25.49199481105542 16.531255250779857 13 24.51452789683894 31.54861609011147 26.583217750949444 17.353638262100148 14 18.486606532518046 34.571395473345085 29.129056732947816 17.812941261189053 15 17.448191435736714 21.603939089388234 43.48277118027617 17.465098294598885 16 21.031088791274392 19.383922412128687 40.08021365260162 19.5047751439953 17 21.754222279274174 19.668625566301497 27.29236655322373 31.284785601200593 18 24.99971300085265 22.81935438759078 31.035774704625695 21.145157906930873 19 29.059655120951877 24.272718069779405 25.245592997638255 21.42203381163046 20 31.115090832621057 23.45513577146936 24.850786534208734 20.57898686170085 21 26.264596515725984 25.64039946106778 27.527810217378374 20.56719380582786 22 25.039997244808188 22.434984256792227 27.62737283067781 24.89764566772178 23 23.189322379358604 26.788917867105827 26.417801878748598 23.603957874786968 24 23.756537057851723 22.687230416482727 33.189103216373354 20.3671293092922 25 21.232614374378386 24.22836365609779 31.166228862512796 23.372793107011024 26 20.21632430277471 29.439224538740188 27.314595941727692 23.02985521675741 27 21.433200687545593 27.300089439370645 28.681024973100357 22.58568489998341 28 18.620400316847057 26.2054225097084 34.90316648768356 20.27101068576098 29 20.547886590460564 23.95127902474559 33.616471037611504 21.884363347182347 30 22.943442382572997 25.62203151563728 29.709316827229642 21.725209274560083 31 29.233106969278566 23.663236244130868 25.419566662596498 21.684090123994068 32 30.146703527793516 24.768026416445153 25.255611876963986 19.829658178797338 33 28.29174976596524 24.70102516095434 25.805710969942318 21.2015141031381 34 22.429348637171504 25.76427872939738 28.701062731751815 23.10530990167931 35 23.603540421481732 25.148639467496565 29.426909666235645 21.82091044478606 36 29.30177803799034 24.787855448443995 25.95902069629124 19.951345817274426 37 24.391587898446137 28.443389679093205 26.497118006743957 20.667904415716702 38 24.126400686293234 29.555485284249173 23.709677924338678 22.60843610511892 39 23.003764385179988 28.486909186164343 25.78473394135407 22.724592487301592 40 24.98677194839025 24.321873196471266 25.852152650150128 24.839202204988357 41 20.55279166679712 24.305592517566957 26.63999140046191 28.50162441517401 42 23.345658642170505 26.01798597565621 24.559612853804722 26.076742528368563 43 23.08965540273286 25.406521246807788 26.052425873338404 25.451397477120953 44 22.464832168116793 27.4160370949007 27.226722020974943 22.89240871600756 45 20.745759457143723 29.74939234453256 24.793386704738406 24.711461493585308 46 24.328656812681395 27.68675556334802 26.632164150988686 21.3524234729819 47 23.33501358288692 25.199986224040927 28.078222400335633 23.38677779273652 48 24.29171219516777 22.591737972909367 29.614033110308906 23.502516721613958 49 22.599147769077355 22.8734145906192 29.9007191676816 24.626718472621846 50 21.2403372605253 27.98606958320418 27.700844612399823 23.072748543870688 51 21.397090976642446 28.913963917423562 25.52340817227463 24.165536933659364 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 481.0 1 898.5 2 1316.0 3 5470.0 4 9624.0 5 6590.5 6 3557.0 7 3621.5 8 3686.0 9 3912.0 10 4138.0 11 4018.0 12 3898.0 13 3839.0 14 3780.0 15 3551.5 16 3323.0 17 3134.0 18 2945.0 19 3004.0 20 3063.0 21 2986.5 22 2910.0 23 3137.0 24 3364.0 25 4030.0 26 5633.0 27 6570.0 28 7331.0 29 8092.0 30 9271.0 31 10450.0 32 11636.5 33 12823.0 34 15335.5 35 17848.0 36 19409.0 37 20970.0 38 25104.5 39 29239.0 40 40901.5 41 52564.0 42 60622.5 43 68681.0 44 72302.5 45 75924.0 46 74434.0 47 72944.0 48 73738.0 49 74532.0 50 72378.5 51 70225.0 52 66385.5 53 62546.0 54 58423.5 55 54301.0 56 51685.0 57 49069.0 58 49010.0 59 48951.0 60 47367.0 61 45783.0 62 41623.0 63 37463.0 64 33165.5 65 28868.0 66 25548.0 67 22228.0 68 18469.0 69 14710.0 70 11818.5 71 8927.0 72 7728.0 73 6529.0 74 5025.5 75 2582.5 76 1643.0 77 1406.0 78 1169.0 79 801.0 80 433.0 81 321.0 82 209.0 83 154.0 84 99.0 85 78.0 86 57.0 87 36.5 88 16.0 89 9.5 90 3.0 91 4.5 92 6.0 93 9.0 94 12.0 95 8.0 96 4.0 97 2.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 958191.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.378262587347873 #Duplication Level Percentage of deduplicated Percentage of total 1 78.28572213737704 18.301841689677527 2 5.804338573483649 2.713907026335458 3 2.0316474552781 1.4248916308322555 4 1.1164993015750373 1.0440725540324691 5 0.8438937207656313 0.9864384499936476 6 0.6280155155117518 0.8809146978337425 7 0.516985123926744 0.8460349786638403 8 0.4685025780612975 0.8762221034213166 9 0.41260117588507184 0.8681308770320741 >10 8.715763725946786 53.41448254020016 >50 1.124879414473231 16.028291117877465 >100 0.049356496041456574 1.7923783396624975 >500 8.973908371173921E-4 0.14245038832221407 >1k 4.4869541855869604E-4 0.10730982861091679 >5k 4.4869541855869604E-4 0.5726337775043937 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5459 0.5697193983245512 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC 1023 0.10676368281480415 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.04351950707113717 0.0 2 0.0 0.0 0.0 0.15226609308582525 0.0 3 0.0 0.0 0.0 0.22427678823950548 0.0 4 0.0 0.0 0.0 0.3583836625474462 0.0 5 0.0 0.0 0.0 0.6783616210129296 0.0 6 0.0 0.0 0.0 1.0179598848246332 0.0 7 0.0 0.0 0.0 1.2374359600538931 0.0 8 0.0 0.0 0.0 1.791918312737231 0.0 9 0.0 0.0 0.0 2.0619062379003767 0.0 10 1.0436332630968147E-4 0.0 0.0 2.5118165376214137 0.0 11 1.0436332630968147E-4 0.0 0.0 2.8307508628238 0.0 12 1.0436332630968147E-4 0.0 0.0 3.1616869705518003 0.0 13 1.0436332630968147E-4 0.0 0.0 3.2818091591342435 0.0 14 1.0436332630968147E-4 0.0 0.0 3.3336777323101554 0.0 15 1.0436332630968147E-4 0.0 0.0 3.408088783968958 0.0 16 1.0436332630968147E-4 0.0 0.0 3.547935641223931 0.0 17 1.0436332630968147E-4 0.0 0.0 3.697070834520466 0.0 18 1.0436332630968147E-4 0.0 0.0 3.916964363054965 0.0 19 1.0436332630968147E-4 0.0 0.0 4.043557077868608 0.0 20 1.0436332630968147E-4 0.0 0.0 4.1779770421554785 0.0 21 1.0436332630968147E-4 0.0 0.0 4.339322744630246 0.0 22 1.0436332630968147E-4 0.0 0.0 4.50839133325193 0.0 23 1.0436332630968147E-4 0.0 0.0 4.684765354715291 0.0 24 1.0436332630968147E-4 0.0 0.0 4.817411142454897 0.0 25 1.0436332630968147E-4 0.0 0.0 4.932523891374475 0.0 26 1.0436332630968147E-4 0.0 0.0 5.0568206130093065 0.0 27 1.0436332630968147E-4 0.0 0.0 5.176107894981272 0.0 28 1.0436332630968147E-4 0.0 0.0 5.292890457121805 0.0 29 1.0436332630968147E-4 0.0 0.0 5.415830455514611 0.0 30 1.0436332630968147E-4 0.0 0.0 5.607024069313947 0.0 31 1.0436332630968147E-4 0.0 0.0 5.760751248968107 0.0 32 1.0436332630968147E-4 0.0 0.0 5.910408258896191 0.0 33 1.0436332630968147E-4 0.0 0.0 6.063404895266184 0.0 34 1.0436332630968147E-4 0.0 0.0 6.226420410961906 0.0 35 1.0436332630968147E-4 0.0 0.0 6.407803872088133 0.0 36 1.0436332630968147E-4 0.0 0.0 6.573011017636358 0.0 37 1.0436332630968147E-4 0.0 0.0 6.731330183648145 0.0 38 2.0872665261936294E-4 0.0 0.0 6.927846327089275 0.0 39 2.0872665261936294E-4 0.0 0.0 7.177483403622033 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTAACG 20 7.032698E-4 45.0 1 CCGATTA 20 7.032698E-4 45.0 11 CACTCGA 20 7.032698E-4 45.0 38 CACTACG 20 7.032698E-4 45.0 1 TCGAGTA 20 7.032698E-4 45.0 35 TATCGCG 20 7.032698E-4 45.0 1 TACGTAG 25 3.8903177E-5 45.0 1 CGTTTTT 3010 0.0 43.056477 1 ACTACGG 60 3.6379788E-12 41.250004 2 ATTAGCG 55 6.002665E-11 40.909092 1 GCGTAAG 45 1.927765E-8 40.0 1 GGGCGAT 1400 0.0 39.696426 7 TCGTTAG 40 3.457426E-7 39.375 1 ATGGTCG 40 3.457426E-7 39.375 44 ATACGAG 40 3.457426E-7 39.375 1 CTAGACG 35 6.247201E-6 38.571426 1 TAGGGCG 310 0.0 38.46774 5 ATAGGGA 965 0.0 38.238342 4 TATAGCG 65 9.094947E-12 38.076927 1 TCACGAC 95 0.0 37.894737 25 >>END_MODULE