FastQCFastQC Report
Thu 26 May 2016
SRR1547065_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547065_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1033746
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69600.6732795096667847No Hit
GAATCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTC15810.15293892310103255No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCG13960.1350428441802919No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGC12480.12072598104369932No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC10520.10176581094388758No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAT207.032958E-445.025
TAACGCC406.8139343E-945.012
GTTACGT207.032958E-445.025
CGTTTTT36900.042.51
GTATGCG700.041.7857131
CGGTCTA1000.040.531
GTCGAAT451.9279469E-840.043
TATAGCG800.039.3751
GGGCGAT12850.038.69657
TCACGAC1050.038.5714325
GACGTAG356.2476793E-638.5714261
GCGTAAG1400.038.5714261
AACGGGA3100.038.467744
CGATGAA5050.038.31683319
CCGATGA5000.038.2518
TAGGGCA4850.038.0412375
TCTAACG301.1398422E-437.5000041
GTTTTTT46600.036.9366952
AGGGCGA7800.036.9230776
TTAGGGA8250.036.818184