##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547065_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1033746
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.66012250591538	31.0	31.0	33.0	30.0	34.0
2	32.04774383649369	33.0	31.0	34.0	30.0	34.0
3	32.137548295229195	33.0	31.0	34.0	30.0	34.0
4	35.82840078704053	37.0	35.0	37.0	35.0	37.0
5	35.76839378338586	37.0	35.0	37.0	35.0	37.0
6	35.8276181963461	37.0	35.0	37.0	35.0	37.0
7	36.09322115877595	37.0	35.0	37.0	35.0	37.0
8	36.0218496613288	37.0	35.0	37.0	35.0	37.0
9	37.80456127520687	39.0	38.0	39.0	35.0	39.0
10	37.42806259951671	39.0	37.0	39.0	35.0	39.0
11	37.1322926521602	39.0	37.0	39.0	33.0	39.0
12	36.42651869995144	38.0	35.0	39.0	33.0	39.0
13	36.12829166932689	38.0	35.0	39.0	32.0	39.0
14	37.13569774393323	39.0	35.0	41.0	32.0	41.0
15	37.31793690132779	39.0	35.0	41.0	32.0	41.0
16	37.46257107645398	39.0	35.0	41.0	33.0	41.0
17	37.41276773985099	39.0	35.0	41.0	33.0	41.0
18	37.32606946000275	39.0	35.0	41.0	33.0	41.0
19	37.262644788952024	39.0	35.0	41.0	32.0	41.0
20	37.06428368283892	39.0	35.0	41.0	32.0	41.0
21	36.996256333760904	38.0	35.0	41.0	32.0	41.0
22	36.91901879185022	38.0	35.0	41.0	32.0	41.0
23	36.86897458369851	38.0	35.0	41.0	32.0	41.0
24	36.80861449524351	38.0	35.0	41.0	32.0	41.0
25	36.725605709719794	38.0	35.0	41.0	32.0	41.0
26	36.55446599067856	38.0	35.0	41.0	31.0	41.0
27	36.428328622311476	38.0	35.0	41.0	31.0	41.0
28	36.467082823053246	38.0	35.0	41.0	31.0	41.0
29	36.48898472158538	38.0	35.0	41.0	31.0	41.0
30	36.42802487264763	38.0	35.0	40.0	31.0	41.0
31	36.29836246041097	38.0	35.0	40.0	31.0	41.0
32	36.16353533653334	38.0	35.0	41.0	30.0	41.0
33	36.040871742188116	38.0	35.0	41.0	30.0	41.0
34	35.85291744780633	38.0	35.0	41.0	30.0	41.0
35	35.75632118528149	38.0	35.0	41.0	30.0	41.0
36	35.65323493391994	38.0	35.0	41.0	29.0	41.0
37	35.56167665945019	38.0	35.0	40.0	29.0	41.0
38	35.48436269644574	37.0	35.0	40.0	29.0	41.0
39	35.39156330471896	37.0	35.0	40.0	29.0	41.0
40	35.24986311918015	37.0	34.0	40.0	28.0	41.0
41	35.20757033159016	37.0	34.0	40.0	28.0	41.0
42	35.09927390287363	37.0	34.0	40.0	27.0	41.0
43	34.97522989206246	36.0	34.0	40.0	27.0	41.0
44	34.853994114608426	36.0	34.0	40.0	27.0	41.0
45	34.768805876878844	36.0	34.0	40.0	26.0	41.0
46	34.73643525585589	36.0	34.0	40.0	26.0	41.0
47	34.67665945019376	35.0	34.0	40.0	26.0	41.0
48	34.61167249982104	35.0	34.0	40.0	26.0	41.0
49	34.5502222015853	35.0	34.0	40.0	26.0	41.0
50	34.43703288815628	35.0	34.0	40.0	26.0	41.0
51	34.086726333161145	35.0	33.0	39.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	5.0
13	6.0
14	14.0
15	27.0
16	78.0
17	186.0
18	421.0
19	722.0
20	1407.0
21	2470.0
22	3748.0
23	5403.0
24	7330.0
25	10418.0
26	13806.0
27	16350.0
28	17767.0
29	19186.0
30	22552.0
31	27536.0
32	34645.0
33	46513.0
34	87786.0
35	146636.0
36	72236.0
37	95227.0
38	140034.0
39	261004.0
40	230.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.64680879055397	22.44119928880015	28.566495057780152	14.345496862865733
2	31.97303786423357	25.200290980569694	29.213172287970156	13.613498867226573
3	29.167706573955304	25.706314703998856	31.866338539641266	13.259640182404574
4	26.609631379468457	26.403487897413875	31.953400545201628	15.033480177916045
5	24.405898547612274	31.021063201211902	29.396873119702523	15.1761651314733
6	22.279554165143082	40.44649265873822	26.187961065871114	11.085992110247584
7	85.90688621769758	3.8657465180034554	8.110019289071008	2.1173479752279576
8	87.08773721978126	3.6241010847925894	7.084332128008235	2.203829567417915
9	82.74721256478864	4.466280885246472	8.589440733023393	4.1970658169414925
10	51.62496396600326	22.315830000793234	13.646195487092575	12.413010546110941
11	42.75566725288417	20.87592116438661	20.614348205458594	15.754063377270624
12	36.716562869408925	19.754949475016108	25.602710917382026	17.92577673819294
13	25.490497665770896	29.163353473677283	27.10820646464412	18.237942395907698
14	19.618842539656743	32.529557550887745	29.53298005506188	18.318619854393635
15	18.786723237623168	22.07795725448998	41.07498360332229	18.060335904564564
16	22.348042942850565	19.361139003198076	38.192650805903966	20.098167248047393
17	22.99442996635537	20.029581734778176	27.32324961837821	29.652738680488245
18	25.265877691425164	22.74872163955169	30.535063739061624	21.45033692996152
19	28.871599019488347	23.963333352680447	25.27738922327148	21.887678404559725
20	31.35634865818102	23.613053883642596	24.023889814325763	21.006707643850618
21	26.71120371928888	25.60387174412283	27.039234009127966	20.645690527460324
22	25.315599770156304	21.368401909173045	28.19773909645116	25.118259224219493
23	24.42253706423048	25.835650150036855	26.26631687087544	23.475495914857227
24	24.047976969197464	23.463790911887447	31.054243498886574	21.43398862002852
25	22.469252601702934	23.519607331007812	30.106234993895985	23.90490507339327
26	21.051496208933333	27.963929243740726	27.21829153389711	23.76628301342883
27	22.01053256796157	26.408227939938822	28.96359453869713	22.61764495340248
28	19.584985093049937	25.94737972383932	33.53280206162829	20.934833121482452
29	21.71258703782167	23.665581293664015	32.87045367043741	21.751377998076897
30	23.528700473810783	24.594436157431325	30.54802630433395	21.32883706442395
31	29.107633790118655	22.80860095226487	25.42965099744038	22.654114260176097
32	30.17839972294935	24.367494529603984	25.575141282287913	19.878964465158752
33	28.20489752801946	24.094893716638325	25.84590411958063	21.85430463576159
34	22.822434137592793	25.538381768829094	28.746810144851832	22.892373948726284
35	23.964397443859518	25.092527564798317	28.275901430332016	22.667173561010152
36	28.41123448119751	25.079274792840796	25.22292710201539	21.286563623946307
37	24.722804247851986	26.893550253156967	26.31168584932856	22.07195964966249
38	24.16715518125342	29.089931182321383	22.68448922656049	24.058424409864706
39	24.436950662928805	26.329968870496234	24.826408034468816	24.40667243210615
40	24.81770183391278	23.49368220046317	25.57697925796085	26.111636707663198
41	21.55248968315234	23.468240747727197	26.361311192497965	28.6179583766225
42	24.127396865380856	24.877387675502494	24.969479930273007	26.02573552884364
43	23.893393541546956	24.537942589378822	25.751296740205042	25.81736712886918
44	23.070077175631152	26.417224347180063	26.968617049062342	23.544081428126447
45	21.934498416438856	27.84697594960464	25.214027430335882	25.004498203620617
46	24.289815873531793	26.488905398424755	26.97606568731584	22.245213040727606
47	23.64168760991578	24.21165353965094	28.005138593039298	24.141520257393985
48	24.29358856043941	23.12463603244898	28.457764286391434	24.124011120720176
49	23.233463539399427	22.105817096269295	29.73012712987523	24.930592234456046
50	21.87345827698487	26.6527754400017	27.093309188137127	24.380457094876302
51	21.305717265169587	27.682041816848624	26.02854086013392	24.983700057847866
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	439.0
1	986.5
2	1534.0
3	6719.0
4	11904.0
5	8087.5
6	4271.0
7	4245.0
8	4219.0
9	4284.5
10	4350.0
11	4326.5
12	4303.0
13	4124.0
14	3945.0
15	3766.5
16	3588.0
17	3428.5
18	3269.0
19	3089.0
20	2909.0
21	3023.5
22	3138.0
23	3216.0
24	3294.0
25	3670.5
26	4877.0
27	5707.0
28	6622.5
29	7538.0
30	8597.0
31	9656.0
32	11460.5
33	13265.0
34	14224.0
35	15183.0
36	18210.0
37	21237.0
38	24051.0
39	26865.0
40	36762.5
41	46660.0
42	57046.5
43	67433.0
44	70249.5
45	73066.0
46	75635.5
47	78205.0
48	77320.0
49	76435.0
50	77122.0
51	77809.0
52	74596.5
53	71384.0
54	65947.5
55	60511.0
56	60357.5
57	60204.0
58	58956.5
59	57709.0
60	56395.5
61	55082.0
62	50040.0
63	44998.0
64	40975.0
65	36952.0
66	31712.0
67	26472.0
68	21650.0
69	16828.0
70	14205.0
71	11582.0
72	9802.5
73	8023.0
74	6354.0
75	3647.5
76	2610.0
77	1992.0
78	1374.0
79	948.0
80	522.0
81	412.5
82	303.0
83	224.5
84	146.0
85	95.5
86	45.0
87	30.5
88	16.0
89	12.5
90	9.0
91	9.0
92	9.0
93	8.0
94	7.0
95	4.5
96	2.0
97	3.0
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1033746.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.776137453215433
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.24449164306117	18.365756508091728
2	5.930682928804018	2.8201746501336524
3	2.1748922894543434	1.5513161406001463
4	1.255358762846311	1.1939032999412946
5	0.9458947635259993	1.1244861956935435
6	0.7075128258672795	1.009315331863999
7	0.5776372062004478	0.9613787128899234
8	0.5311456661011776	1.0102873891921016
9	0.46530047861668006	0.99567433229734
>10	9.31799900025107	56.41797171833658
>50	0.8077156328059758	11.602178430861326
>100	0.03768247353300339	1.5498851644337113
>500	0.0016383684144784083	0.20596965898694938
>1k	0.0016383684144784083	0.5139016030610553
>5k	4.0959210361960207E-4	0.6778008636166278
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6960	0.6732795096667847	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTC	1581	0.15293892310103255	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCG	1396	0.1350428441802919	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGC	1248	0.12072598104369932	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1052	0.10176581094388758	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.673556173373344E-5	0.0	0.0	0.03569542227974764	0.0
2	9.673556173373344E-5	0.0	0.0	0.1275942059267944	0.0
3	9.673556173373344E-5	0.0	0.0	0.19405153683786927	0.0
4	9.673556173373344E-5	0.0	0.0	0.3312225633763033	0.0
5	9.673556173373344E-5	0.0	0.0	0.712360676607213	0.0
6	9.673556173373344E-5	0.0	0.0	1.0572229541879727	0.0
7	9.673556173373344E-5	0.0	0.0	1.3138623994675676	0.0
8	9.673556173373344E-5	0.0	0.0	1.8742515085910851	0.0
9	9.673556173373344E-5	0.0	0.0	2.176937081255937	0.0
10	9.673556173373344E-5	0.0	0.0	2.70637081062466	0.0
11	9.673556173373344E-5	0.0	0.0	3.027242668895454	0.0
12	9.673556173373344E-5	0.0	0.0	3.4306299613251223	0.0
13	9.673556173373344E-5	0.0	0.0	3.56944549241303	0.0
14	9.673556173373344E-5	0.0	0.0	3.6323236075399565	0.0
15	9.673556173373344E-5	0.0	0.0	3.718514993044713	0.0
16	1.9347112346746687E-4	0.0	0.0	3.852880688292869	0.0
17	1.9347112346746687E-4	0.0	0.0	4.000208948813345	0.0
18	0.004546571401485471	0.0	0.0	4.202579743960316	0.0
19	0.004546571401485471	0.0	0.0	4.340814861677821	0.0
20	0.004546571401485471	0.0	0.0	4.474019730185171	0.0
21	0.004546571401485471	0.0	0.0	4.620767577335245	0.0
22	0.004546571401485471	0.0	0.0	4.7866690657085975	0.0
23	0.004546571401485471	0.0	0.0	4.958084481100773	0.0
24	0.004546571401485471	0.0	0.0	5.091869762978527	0.0
25	0.004546571401485471	0.0	0.0	5.197214789706562	0.0
26	0.004546571401485471	0.0	0.0	5.306139032218746	0.0
27	0.004546571401485471	0.0	0.0	5.432378940281268	0.0
28	0.004546571401485471	0.0	0.0	5.55213756570763	0.0
29	0.004546571401485471	0.0	0.0	5.689695534493	0.0
30	0.004546571401485471	0.0	0.0	5.880071119984986	0.0
31	0.004643306963219205	0.0	0.0	6.027109173820262	0.0
32	0.004643306963219205	0.0	0.0	6.176468881137146	0.0
33	0.004643306963219205	0.0	0.0	6.324184083904557	0.0
34	0.004643306963219205	0.0	0.0	6.4768328003203886	0.0
35	0.004643306963219205	0.0	0.0	6.647087388971759	0.0
36	0.004643306963219205	0.0	0.0	6.807861892573224	0.0
37	0.004643306963219205	0.0	0.0	6.973279703137909	0.0
38	0.004643306963219205	0.0	0.0	7.195674759563762	0.0
39	0.004643306963219205	0.0	0.0	7.516353146711087	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAT	20	7.032958E-4	45.0	25
TAACGCC	40	6.8139343E-9	45.0	12
GTTACGT	20	7.032958E-4	45.0	25
CGTTTTT	3690	0.0	42.5	1
GTATGCG	70	0.0	41.785713	1
CGGTCTA	100	0.0	40.5	31
GTCGAAT	45	1.9279469E-8	40.0	43
TATAGCG	80	0.0	39.375	1
GGGCGAT	1285	0.0	38.6965	7
TCACGAC	105	0.0	38.57143	25
GACGTAG	35	6.2476793E-6	38.571426	1
GCGTAAG	140	0.0	38.571426	1
AACGGGA	310	0.0	38.46774	4
CGATGAA	505	0.0	38.316833	19
CCGATGA	500	0.0	38.25	18
TAGGGCA	485	0.0	38.041237	5
TCTAACG	30	1.1398422E-4	37.500004	1
GTTTTTT	4660	0.0	36.936695	2
AGGGCGA	780	0.0	36.923077	6
TTAGGGA	825	0.0	36.81818	4
>>END_MODULE