FastQCFastQC Report
Thu 26 May 2016
SRR1547064_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547064_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences872557
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32520.37269771487707964No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC12240.14027736869912225No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCG453.8380676E-1045.0000041
AACGTAG253.8900307E-545.01
TTATACG253.8900307E-545.01
ACATACG207.032352E-445.017
ATAGCGG1300.041.5384642
CGGTCTA650.041.53846431
CGTTTTT24750.041.4545441
GCGGGAT3300.040.909095
TTGCGAG451.9274012E-840.0000041
CGTATGG850.039.7058832
GGACCGA2450.039.4897968
ATGGGCG2000.039.3755
CTAAGCG800.039.3751
TCTAGCG403.456953E-739.3751
TATAGCG403.456953E-739.3751
AGGGCGA5050.038.7623756
CACGACG700.038.5714326
TATGGGC3800.038.4868434
GGGCGAT9950.037.9899527
TAGCGGG6650.037.8947373
Produced by FastQC (version 0.11.5)