##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547064_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	872557
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.685408517724344	31.0	31.0	33.0	30.0	34.0
2	32.0775147067756	33.0	31.0	34.0	30.0	34.0
3	32.1729640585085	33.0	31.0	34.0	30.0	34.0
4	35.82927075251244	37.0	35.0	37.0	35.0	37.0
5	35.76940761463148	37.0	35.0	37.0	35.0	37.0
6	35.82421664143431	37.0	35.0	37.0	35.0	37.0
7	36.13964130710085	37.0	35.0	37.0	35.0	37.0
8	36.08972365129155	37.0	35.0	37.0	35.0	37.0
9	37.89220532297603	39.0	38.0	39.0	35.0	39.0
10	37.436724477598595	39.0	37.0	39.0	35.0	39.0
11	37.13454479191617	39.0	37.0	39.0	33.0	39.0
12	36.267341847008275	38.0	35.0	39.0	33.0	39.0
13	35.87653070229223	38.0	35.0	39.0	31.0	39.0
14	36.83701236710037	39.0	35.0	41.0	31.0	41.0
15	37.11525206949231	39.0	35.0	41.0	32.0	41.0
16	37.2819563650283	39.0	35.0	41.0	33.0	41.0
17	37.27807008596573	39.0	35.0	41.0	33.0	41.0
18	37.207701044172474	39.0	35.0	41.0	33.0	41.0
19	37.1566052418352	38.0	35.0	41.0	32.0	41.0
20	36.994521847856355	38.0	35.0	41.0	32.0	41.0
21	36.89974064731588	38.0	35.0	41.0	32.0	41.0
22	36.82595520980291	38.0	35.0	41.0	32.0	41.0
23	36.804583540101106	38.0	35.0	41.0	32.0	41.0
24	36.71526444690719	38.0	35.0	41.0	32.0	41.0
25	36.62139206951523	38.0	35.0	41.0	32.0	41.0
26	36.48259999060233	38.0	35.0	41.0	31.0	41.0
27	36.39985353392386	38.0	35.0	40.0	31.0	41.0
28	36.48154561822322	38.0	35.0	41.0	31.0	41.0
29	36.51206511437075	38.0	35.0	41.0	31.0	41.0
30	36.49454304990963	38.0	35.0	40.0	31.0	41.0
31	36.33459819816929	38.0	35.0	40.0	31.0	41.0
32	36.23870073817527	38.0	35.0	41.0	31.0	41.0
33	36.12341314091802	38.0	35.0	41.0	31.0	41.0
34	36.00480885489429	38.0	35.0	41.0	30.0	41.0
35	35.92856970948603	38.0	35.0	41.0	30.0	41.0
36	35.81306206929748	38.0	35.0	41.0	30.0	41.0
37	35.73412510586701	37.0	35.0	40.0	30.0	41.0
38	35.67278240848449	37.0	35.0	40.0	30.0	41.0
39	35.63992954041971	37.0	35.0	40.0	30.0	41.0
40	35.49282854873665	37.0	35.0	40.0	29.0	41.0
41	35.48594303867827	37.0	35.0	40.0	29.0	41.0
42	35.42098453166957	37.0	35.0	40.0	29.0	41.0
43	35.29842749528111	36.0	34.0	40.0	29.0	41.0
44	35.17193604543886	36.0	34.0	40.0	28.0	41.0
45	35.07139247063515	36.0	34.0	40.0	28.0	41.0
46	35.03590252556567	36.0	34.0	40.0	28.0	41.0
47	34.977818068045984	35.0	34.0	40.0	28.0	41.0
48	34.92753482007479	35.0	34.0	40.0	28.0	41.0
49	34.87579264162685	36.0	34.0	40.0	28.0	41.0
50	34.74281221742534	35.0	34.0	40.0	28.0	41.0
51	34.366561726053426	35.0	34.0	39.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	1.0
13	7.0
14	13.0
15	26.0
16	62.0
17	144.0
18	297.0
19	587.0
20	1035.0
21	1620.0
22	2462.0
23	3736.0
24	5464.0
25	7730.0
26	10817.0
27	12907.0
28	14363.0
29	15707.0
30	18617.0
31	23165.0
32	29367.0
33	39346.0
34	77058.0
35	143060.0
36	53513.0
37	71818.0
38	109638.0
39	229765.0
40	229.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.104312956059026	23.179918331982897	30.229772954660845	13.485995757297232
2	31.189824848118803	24.56378207956615	30.646937678569998	13.59945539374505
3	29.850428109567627	24.55071703052064	31.852704178638184	13.746150681273544
4	26.262353061175375	27.42239188958429	31.213662832342187	15.101592216898151
5	24.517481379439968	30.697249577964534	29.86601448386753	14.919254558727967
6	21.85668099619853	40.148666505454656	26.487667854363668	11.506984643983143
7	88.30907321813933	4.183680836896615	5.811196288609226	1.6960496563548282
8	89.69740658776448	2.7527141493335106	5.5331628764653775	2.0167163864366455
9	86.33567778380095	3.8430727161663936	7.132943750379632	2.6883057496530314
10	53.015218490024154	20.908318883465494	12.729598180978435	13.346864445531924
11	45.76617917224892	20.425599702942044	18.91773259511986	14.890488529689177
12	40.606172433434146	20.953473526657856	22.40885122691125	16.031502812996745
13	23.605907694282436	35.134208997234566	24.16804862031936	17.091834688163637
14	17.33078755886435	37.49256495564187	27.0347954345676	18.141852050926186
15	16.442822646543434	22.49010666351883	44.625967128795025	16.44110356114271
16	18.983974685894445	19.12024085532521	42.121374305632756	19.77441015314759
17	20.528859432678896	18.958761433350485	27.03834821106243	33.474030922908184
18	24.616615304215085	23.36030769336559	30.608430165593763	21.41464683682556
19	29.02824686524777	24.576159494451367	24.744171440948843	21.65142219935202
20	31.03957678409548	24.11005813946825	23.572328226121616	21.27803685031465
21	24.609165934145278	26.356100518361554	27.25048334951184	21.784250197981336
22	23.898496029485752	23.45692029288631	26.598720771250473	26.045862906377465
23	21.643858223588833	27.507085496993323	26.011022775589442	24.838033503828402
24	22.68780148460215	23.02256471496991	34.248421593087905	20.04121220734004
25	20.693891631148453	23.38632318576322	32.59191090094974	23.327874282138588
26	19.299598765467472	31.471525642450864	26.280804577809818	22.948071014271846
27	19.664274081807836	29.686656573725266	29.160731046796943	21.48833829766995
28	17.79688891384746	26.563766034769078	35.731533871139646	19.90781118024381
29	18.755450933291463	24.48539178529311	34.35110829435784	22.40804898705758
30	21.89175033837331	26.731777981266553	30.352515652272572	21.02395602808756
31	28.901722179754447	24.80147428763966	24.500405131126104	21.796398401479788
32	30.34655615621673	25.469281662974453	25.145979001944855	19.03818317886396
33	28.151398705184878	25.952803083351572	25.36934549834567	20.526452713117884
34	21.931518513976737	26.556660481779414	28.18348829933173	23.328332704912114
35	22.096092289672768	25.658724874134297	29.327826147747366	22.91735668844557
36	29.646544581041695	24.68022146404189	25.148156510119108	20.5250774447973
37	22.71163946882553	29.60689101113165	27.07295913046368	20.60851038957913
38	22.977639283164308	29.87403688240424	23.49474017170225	23.653583662729197
39	22.808252068346253	28.11804844841082	25.733103969138977	23.34059551410395
40	24.42591143042804	24.402531868978187	25.473178256549428	25.698378444044344
41	19.45580632554664	23.839015674620683	26.62828904014294	30.07688895968974
42	24.115100789977046	25.66216304493575	24.07762472824125	26.14511143684596
43	23.782285856396772	25.84129174369124	25.035957536298486	25.340464863613494
44	21.94790712813031	28.811985921836623	26.876066549234036	22.36404040079903
45	20.46605551270576	32.36911743301584	22.65399280505457	24.510834249223834
46	23.203870921899657	29.373668425100025	25.972400656919838	21.450059996080487
47	23.390678202111726	26.40377648680831	26.26567662628344	23.939868684796522
48	24.576044888757984	23.50665916381394	28.53200421290529	23.385291734522788
49	22.840112451106346	22.79266569404635	30.009042389207814	24.35817946563949
50	20.762655047177432	28.847628292478312	27.209110694201065	23.180605966143187
51	20.59865429994831	30.951674217271762	24.339957160391815	24.109714322388108
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	397.0
1	764.5
2	1132.0
3	3456.5
4	5781.0
5	4198.5
6	2616.0
7	2690.0
8	2764.0
9	2976.0
10	3188.0
11	3169.0
12	3150.0
13	2998.5
14	2847.0
15	2654.5
16	2462.0
17	2449.0
18	2436.0
19	2318.0
20	2200.0
21	2279.0
22	2358.0
23	2365.0
24	2372.0
25	2721.0
26	3616.5
27	4163.0
28	4811.0
29	5459.0
30	7297.0
31	9135.0
32	9828.5
33	10522.0
34	12194.0
35	13866.0
36	16313.0
37	18760.0
38	23824.5
39	28889.0
40	39114.5
41	49340.0
42	60331.0
43	71322.0
44	72410.0
45	73498.0
46	74771.0
47	76044.0
48	75402.0
49	74760.0
50	73401.0
51	72042.0
52	67480.0
53	62918.0
54	57939.5
55	52961.0
56	49083.5
57	45206.0
58	43284.0
59	41362.0
60	39052.5
61	36743.0
62	33082.5
63	29422.0
64	25198.0
65	20974.0
66	17652.0
67	14330.0
68	11733.0
69	9136.0
70	7483.0
71	5830.0
72	4817.5
73	3805.0
74	2962.0
75	1985.5
76	1852.0
77	1261.5
78	671.0
79	540.0
80	409.0
81	257.5
82	106.0
83	84.0
84	62.0
85	61.5
86	61.0
87	34.5
88	8.0
89	6.5
90	5.0
91	3.5
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	872557.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.038125359751078
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.77911518530344	17.361440161839866
2	6.108663978781033	2.692470050899444
3	1.9992397135797628	1.3217848629619098
4	1.1361212930949247	1.0015193392443378
5	0.7315325979236398	0.8060803548892777
6	0.5125605734125146	0.6777524502798536
7	0.4245864298012332	0.6549962276206109
8	0.3843605871686046	0.6776469442695392
9	0.3303022732289696	0.6551318613627707
>10	7.741466573005599	47.17473822888826
>50	1.7772151734394197	24.137214929674684
>100	0.07378896921523599	2.3230019356931666
>500	0.0	0.0
>1k	0.0010466520456061843	0.5162226523762592
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3252	0.37269771487707964	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1224	0.14027736869912225	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04836360260705031	0.0
2	0.0	0.0	0.0	0.17293999131288845	0.0
3	0.0	0.0	0.0	0.2568313588682459	0.0
4	0.0	0.0	0.0	0.3874818493233107	0.0
5	0.0	0.0	0.0	0.6675781639480286	0.0
6	0.0	0.0	0.0	0.994089784392309	0.0
7	0.0	0.0	0.0	1.190180125768288	0.0
8	0.0	0.0	0.0	1.693986753873959	0.0
9	0.0	0.0	0.0	1.918040884435057	0.0
10	0.0	0.0	0.0	2.2923430790194796	0.0
11	0.0	0.0	0.0	2.661831834481873	0.0
12	0.0	0.0	0.0	2.9650784991696817	0.0
13	0.0	0.0	0.0	3.097791892105616	0.0
14	0.0	0.0	0.0	3.152229596461893	0.0
15	0.0	0.0	0.0	3.228900805334207	0.0
16	0.0	0.0	0.0	3.394047609497145	0.0
17	0.0	0.0	0.0	3.580854889709211	0.0
18	0.0	0.0	0.0	3.841468236459051	0.0
19	0.0	0.0	0.0	3.9686805561126666	0.0
20	0.0	0.0	0.0	4.114458998094108	0.0
21	0.0	0.0	0.0	4.313758298884772	0.0
22	0.0	0.0	0.0	4.506639680846065	0.0
23	0.0	0.0	0.0	4.741352140891655	0.0
24	0.0	0.0	0.0	4.896184432650245	0.0
25	0.0	0.0	0.0	5.025803471864875	0.0
26	0.0	0.0	0.0	5.154276454145689	0.0
27	1.1460569338163582E-4	0.0	0.0	5.290198806496309	0.0
28	1.1460569338163582E-4	0.0	0.0	5.427496427167509	0.0
29	1.1460569338163582E-4	0.0	0.0	5.574993954549674	0.0
30	1.1460569338163582E-4	0.0	0.0	5.7697090276050735	0.0
31	1.1460569338163582E-4	0.0	0.0	5.934855831768011	0.0
32	1.1460569338163582E-4	0.0	0.0	6.08338481039061	0.0
33	1.1460569338163582E-4	0.0	0.0	6.2269857441978	0.0
34	3.438170801449074E-4	0.0	0.0	6.384453966904168	0.0
35	3.438170801449074E-4	0.0	0.0	6.591317243458021	0.0
36	3.438170801449074E-4	0.0	0.0	6.756922470394485	0.0
37	3.438170801449074E-4	0.0	0.0	6.928372587693412	0.0
38	3.438170801449074E-4	0.0	0.0	7.119878701334125	0.0
39	3.438170801449074E-4	0.0	0.0	7.358831572034836	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGCG	45	3.8380676E-10	45.000004	1
AACGTAG	25	3.8900307E-5	45.0	1
TTATACG	25	3.8900307E-5	45.0	1
ACATACG	20	7.032352E-4	45.0	17
ATAGCGG	130	0.0	41.538464	2
CGGTCTA	65	0.0	41.538464	31
CGTTTTT	2475	0.0	41.454544	1
GCGGGAT	330	0.0	40.90909	5
TTGCGAG	45	1.9274012E-8	40.000004	1
CGTATGG	85	0.0	39.705883	2
GGACCGA	245	0.0	39.489796	8
ATGGGCG	200	0.0	39.375	5
CTAAGCG	80	0.0	39.375	1
TCTAGCG	40	3.456953E-7	39.375	1
TATAGCG	40	3.456953E-7	39.375	1
AGGGCGA	505	0.0	38.762375	6
CACGACG	70	0.0	38.57143	26
TATGGGC	380	0.0	38.486843	4
GGGCGAT	995	0.0	37.989952	7
TAGCGGG	665	0.0	37.894737	3
>>END_MODULE