FastQCFastQC Report
Thu 26 May 2016
SRR1547063_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547063_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1242378
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98950.796456472989702No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC13780.11091632337340165No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCAAG207.0335064E-445.0000041
CGCAACC207.0335064E-445.00000435
TCGACTA207.0335064E-445.00000445
ACGCGTA253.890988E-545.00000432
TAATGCG351.212029E-745.01
CGTTTTT61550.043.2818871
TATAGCG556.184564E-1140.9090921
CGAATAT501.0822987E-940.50000414
TATCGTG501.0822987E-940.5000041
CACGACC1600.039.37500427
GCGATCG403.4585173E-739.3750049
CGTAAGG1600.039.3750042
GCGTAAG750.039.01
TAAGGGA10350.038.6956524
CTATGCG356.248698E-638.5714261
CCGGATA356.248698E-638.57142620
ATCGTAG356.248698E-638.5714261
GCGTTAG301.139975E-437.5000041
GCACCGT301.139975E-437.50000439
TTCTCGT301.139975E-437.50000431