##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547063_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1242378
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.68490427229072	31.0	31.0	33.0	30.0	34.0
2	32.068270687343144	33.0	31.0	34.0	30.0	34.0
3	32.13238241501379	33.0	31.0	34.0	30.0	34.0
4	35.82979012828624	37.0	35.0	37.0	35.0	37.0
5	35.78087264906494	37.0	35.0	37.0	35.0	37.0
6	35.84549307859605	37.0	35.0	37.0	35.0	37.0
7	36.153363147125916	37.0	35.0	37.0	35.0	37.0
8	36.105173304743	37.0	35.0	37.0	35.0	37.0
9	37.89940018255314	39.0	38.0	39.0	35.0	39.0
10	37.411767594081674	39.0	37.0	39.0	35.0	39.0
11	37.156429846632825	39.0	37.0	39.0	34.0	39.0
12	36.47329154250961	38.0	35.0	39.0	33.0	39.0
13	36.175146372521084	39.0	35.0	39.0	32.0	39.0
14	37.21388820471708	39.0	35.0	41.0	32.0	41.0
15	37.431751045173044	39.0	35.0	41.0	33.0	41.0
16	37.55328732479165	39.0	35.0	41.0	33.0	41.0
17	37.5451915600566	39.0	35.0	41.0	33.0	41.0
18	37.42860224504941	39.0	35.0	41.0	33.0	41.0
19	37.35071693156189	39.0	35.0	41.0	33.0	41.0
20	37.16569675251815	39.0	35.0	41.0	33.0	41.0
21	37.09482782212821	39.0	35.0	41.0	33.0	41.0
22	37.0124768790175	38.0	35.0	41.0	32.0	41.0
23	36.99079990147926	38.0	35.0	41.0	32.0	41.0
24	36.90734060004282	38.0	35.0	41.0	32.0	41.0
25	36.81088686374034	38.0	35.0	41.0	32.0	41.0
26	36.694481067758765	38.0	35.0	41.0	32.0	41.0
27	36.612185663300544	38.0	35.0	41.0	32.0	41.0
28	36.634609595469335	38.0	35.0	41.0	32.0	41.0
29	36.66176638671966	38.0	35.0	41.0	32.0	41.0
30	36.60265152795687	38.0	35.0	41.0	32.0	41.0
31	36.44367817202172	38.0	35.0	41.0	31.0	41.0
32	36.29428322137063	38.0	35.0	41.0	31.0	41.0
33	36.1161578843154	38.0	35.0	41.0	30.0	41.0
34	35.92679844620558	38.0	35.0	41.0	30.0	41.0
35	35.823219664224574	38.0	35.0	41.0	30.0	41.0
36	35.69429915854917	38.0	35.0	41.0	29.0	41.0
37	35.60165102730409	38.0	35.0	41.0	29.0	41.0
38	35.52128015789076	38.0	35.0	41.0	29.0	41.0
39	35.47320219772082	38.0	35.0	40.0	29.0	41.0
40	35.35408708138747	38.0	35.0	40.0	27.0	41.0
41	35.34485961599449	38.0	35.0	40.0	28.0	41.0
42	35.25355970566124	37.0	35.0	40.0	27.0	41.0
43	35.14560705356985	37.0	34.0	40.0	27.0	41.0
44	35.006238841962755	37.0	34.0	40.0	27.0	41.0
45	34.91797343481613	37.0	34.0	40.0	26.0	41.0
46	34.90230751027465	37.0	34.0	40.0	26.0	41.0
47	34.87633876324275	36.0	34.0	40.0	26.0	41.0
48	34.81670795844743	36.0	34.0	40.0	26.0	41.0
49	34.75287392403922	36.0	34.0	40.0	26.0	41.0
50	34.64422422161371	36.0	34.0	40.0	26.0	41.0
51	34.27070102657967	35.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	3.0
13	5.0
14	12.0
15	19.0
16	56.0
17	175.0
18	377.0
19	759.0
20	1406.0
21	2313.0
22	3525.0
23	5613.0
24	8337.0
25	13089.0
26	18098.0
27	21063.0
28	21724.0
29	23082.0
30	26200.0
31	31718.0
32	39888.0
33	53424.0
34	100026.0
35	179816.0
36	80367.0
37	102995.0
38	159955.0
39	348081.0
40	250.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.37364151651108	23.298062264463795	29.393389129556386	14.934907089468744
2	32.474737962198304	24.590503051406255	30.062187192625757	12.87257179376969
3	28.533505905610046	24.283108683508562	33.6088533441513	13.574532066730093
4	25.25962307767845	27.18415812256817	33.11150068658653	14.444718113166847
5	23.436667423280195	31.203144292638797	31.43326749185836	13.926920792222658
6	21.293519363671926	40.821070559845715	27.54821801416316	10.337192062319197
7	86.38916658215132	3.6711049294176163	8.267451612955155	1.6722768754759019
8	87.83655216045358	2.4881316314358433	7.7230923277778585	1.952223880332717
9	83.51773775775166	3.9401051853783633	9.698658540315428	2.843498516554543
10	47.48554787673317	23.833648052364094	17.527998725025718	11.152805345877018
11	41.08306811614501	21.352358138988293	22.865424210666962	14.699149534199737
12	35.82645539441297	21.329579242388387	26.302300910028993	16.541664453169645
13	22.928448507620065	33.00959933289224	27.45090463610914	16.611047523378552
14	17.347135895838463	35.20039794651869	30.34961984194826	17.102846315694578
15	17.18945441725465	23.54452509622675	43.27233740455803	15.993683081960564
16	19.632350218693507	20.711329402162626	40.88320945799105	18.77311092115282
17	20.39282730376745	20.7008655980708	29.512917968605368	29.393389129556386
18	23.458882884275155	23.190848517922888	33.279484987660766	20.070783610141195
19	27.026718116386476	25.39452565966236	27.217883768064148	20.360872455887016
20	28.77183916650166	24.72170305655767	26.996292593719463	19.510165183221208
21	24.032862784112403	26.582086933284394	29.32859403498774	20.056456247615458
22	22.947846790590305	23.247996986424422	29.137106420107244	24.66704980287803
23	21.308893106606845	27.63780427534937	28.069476439537727	22.98382617850606
24	21.350184887369224	23.92178547913759	35.414503476397684	19.313526157095506
25	19.14530038361916	25.768566410544942	33.13081847875606	21.95531472707984
26	18.97989178816753	31.0494873540903	28.55990688824174	21.410713969500424
27	19.062475349692285	29.04228825687512	31.023569316262844	20.87166707716975
28	17.05873735690748	27.086683762912738	36.619048308968765	19.235530571211015
29	18.5573955752597	24.80565496169443	35.08119107067253	21.555758392373335
30	20.654502896863917	27.136588059350697	32.325347036087244	19.883562007698142
31	26.861148539333442	25.377864063916135	27.12869996088147	20.632287435868953
32	27.481491140377567	25.722686654142297	27.495094085696948	19.30072811978319
33	25.750616961987415	26.94485897206808	27.12161677041931	20.182907295525194
34	20.298170122136742	27.493323288081406	29.57706913676836	22.631437453013493
35	20.973890394066864	27.432230770345257	30.043915780865404	21.549963054722475
36	27.267868555302815	26.066784827162103	27.06535370072554	19.59999291680954
37	22.35438811698211	29.663757729129138	28.239633992231028	19.742220161657727
38	22.351812411359507	30.520099357844394	25.19128638787873	21.936801842917372
39	22.50852800033484	29.123986419592107	26.963291365429843	21.40419421464321
40	23.92532707436867	26.141802253420455	26.71546019005488	23.217410482155994
41	20.09404545154534	25.310573754525596	27.711292376394304	26.884088417534755
42	22.50321560748822	26.988887440054476	25.55800247589703	24.949894476560274
43	22.594250703087145	26.344558580399845	26.85841185210942	24.202778864403587
44	21.250537276094718	28.96839770182666	27.617198630368534	22.16386639171009
45	20.285372084824427	31.909048614833814	24.726210541397222	23.07936875894454
46	22.907681881037817	29.32883550738986	27.068734314355215	20.69474829721711
47	21.975437427256438	26.32419440782113	28.53374737801217	23.166620786910265
48	23.579055649729792	23.89570645970872	30.023390626685277	22.50184726387621
49	22.16121019528678	23.63523822862285	30.371191376537578	23.832360199552795
50	20.392505340564625	28.85780334165608	28.175321842466623	22.57436947531267
51	19.99117820824258	30.302210760332205	26.10719120911671	23.59941982230851
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	775.0
1	1058.5
2	1342.0
3	8973.5
4	16605.0
5	11502.0
6	6399.0
7	6408.5
8	6418.0
9	6655.5
10	6893.0
11	6993.5
12	7094.0
13	6824.5
14	6555.0
15	6257.0
16	5959.0
17	5635.0
18	5311.0
19	5072.5
20	4834.0
21	4948.0
22	5062.0
23	5396.0
24	5730.0
25	6659.5
26	8874.5
27	10160.0
28	11634.0
29	13108.0
30	14836.5
31	16565.0
32	18820.0
33	21075.0
34	24650.0
35	28225.0
36	30998.5
37	33772.0
38	40465.0
39	47158.0
40	59545.0
41	71932.0
42	85059.0
43	98186.0
44	104424.5
45	110663.0
46	108636.5
47	106610.0
48	104909.0
49	103208.0
50	98835.0
51	94462.0
52	87885.0
53	81308.0
54	74195.0
55	67082.0
56	62457.5
57	57833.0
58	53591.5
59	49350.0
60	46463.0
61	43576.0
62	39557.5
63	35539.0
64	29858.0
65	24177.0
66	20042.0
67	15907.0
68	12547.0
69	9187.0
70	7975.5
71	6764.0
72	5777.5
73	4791.0
74	3690.5
75	1866.5
76	1143.0
77	985.0
78	827.0
79	607.0
80	387.0
81	269.5
82	152.0
83	96.0
84	40.0
85	26.5
86	13.0
87	11.5
88	10.0
89	7.0
90	4.0
91	5.0
92	6.0
93	4.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1242378.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.400423898341973
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.40319344839595	19.130711551245692
2	6.8472192657570154	3.3415010521873003
3	2.365906034975154	1.7318733047111778
4	1.330218942224921	1.2983162427156858
5	0.906611466933693	1.1060852052139878
6	0.6893275647743282	1.0091930871183235
7	0.5445871279804415	0.9301709740612366
8	0.45689721373283304	0.8918788554441984
9	0.40637397122056645	0.8924127443130998
>10	6.302763484592429	39.34938153270274
>50	1.6527531359354257	26.16847212252654
>100	0.09281762484161486	3.1455408213504725
>500	6.653593178606485E-4	0.08937394694981571
>1k	3.3267965893032426E-4	0.11185949036952411
>5k	3.3267965893032426E-4	0.8032290690903057
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9895	0.796456472989702	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1378	0.11091632337340165	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04555779319981519	0.0
2	0.0	0.0	0.0	0.160659638209949	0.0
3	0.0	0.0	0.0	0.25121178900463464	0.0
4	0.0	0.0	0.0	0.3761335117009477	0.0
5	0.0	0.0	0.0	0.6937502112883519	0.0
6	0.0	0.0	0.0	1.100953172061965	0.0
7	0.0	0.0	0.0	1.3810611585201926	0.0
8	0.0	0.0	0.0	2.02233136774798	0.0
9	0.0	0.0	0.0	2.3702930992016924	0.0
10	0.0	0.0	0.0	2.8619309099163055	0.0
11	0.0	0.0	0.0	3.268489944284268	0.0
12	0.0	0.0	0.0	3.6174175653464564	0.0
13	0.0	0.0	0.0	3.7900703328616574	0.0
14	0.0	0.0	0.0	3.8566362250458397	0.0
15	0.0	0.0	0.0	3.9491201550574786	0.0
16	0.0	0.0	0.0	4.130546419849676	0.0
17	0.0	0.0	0.0	4.335234526046018	0.0
18	0.0	0.0	0.0	4.6273356418094975	0.0
19	0.0	0.0	0.0	4.787270862813089	0.0
20	0.0	0.0	0.0	4.951391605453413	0.0
21	0.0	0.0	0.0	5.159379834478718	0.0
22	0.0	0.0	0.0	5.372036529944992	0.0
23	0.0	0.0	0.0	5.60513788879069	0.0
24	0.0	0.0	0.0	5.7889788776040785	0.0
25	0.0	0.0	0.0	5.949960479016853	0.0
26	0.0	0.0	0.0	6.094924411089057	0.0
27	0.0	0.0	0.0	6.258723190526554	0.0
28	0.0	0.0	0.0	6.426627000800079	0.0
29	0.0	0.0	0.0	6.608214247193688	0.0
30	0.0	0.0	0.0	6.849847630914263	0.0
31	0.0	0.0	0.0	7.048659908659039	0.0
32	0.0	0.0	0.0	7.232661879073841	0.0
33	0.0	0.0	0.0	7.424149493954336	0.0
34	0.0	0.0	0.0	7.615959072037657	0.0
35	0.0	0.0	0.0	7.839643007200707	0.0
36	0.0	0.0	0.0	8.044653076599875	0.0
37	0.0	0.0	0.0	8.255136520447078	0.0
38	0.0	0.0	0.0	8.497655302975422	8.049080070638727E-5
39	0.0	0.0	0.0	8.782109792671795	8.049080070638727E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCAAG	20	7.0335064E-4	45.000004	1
CGCAACC	20	7.0335064E-4	45.000004	35
TCGACTA	20	7.0335064E-4	45.000004	45
ACGCGTA	25	3.890988E-5	45.000004	32
TAATGCG	35	1.212029E-7	45.0	1
CGTTTTT	6155	0.0	43.281887	1
TATAGCG	55	6.184564E-11	40.909092	1
CGAATAT	50	1.0822987E-9	40.500004	14
TATCGTG	50	1.0822987E-9	40.500004	1
CACGACC	160	0.0	39.375004	27
GCGATCG	40	3.4585173E-7	39.375004	9
CGTAAGG	160	0.0	39.375004	2
GCGTAAG	75	0.0	39.0	1
TAAGGGA	1035	0.0	38.695652	4
CTATGCG	35	6.248698E-6	38.571426	1
CCGGATA	35	6.248698E-6	38.571426	20
ATCGTAG	35	6.248698E-6	38.571426	1
GCGTTAG	30	1.139975E-4	37.500004	1
GCACCGT	30	1.139975E-4	37.500004	39
TTCTCGT	30	1.139975E-4	37.500004	31
>>END_MODULE