FastQCFastQC Report
Thu 26 May 2016
SRR1547062_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547062_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences959161
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75070.782663181676486No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATAGCG453.8380676E-1045.01
ATCGCTA253.8903218E-545.011
ATATGCG302.165125E-644.9999961
CGTTTTT39050.042.4071731
GACGTAG700.041.7857131
CGTTAGG1100.040.9090922
AATACGG556.002665E-1140.9090922
ATAGGGC4250.039.1764684
GCGTAAG1150.039.1304321
GCGGGAT3050.039.0983625
AATGCGG1500.039.02
CGTAAGG1750.038.571432
TGTTACG356.24721E-638.5714261
GTATGCG659.094947E-1238.076921
GGGCGAT10400.038.076927
GTTGACG301.139781E-437.4999961
ACGCATA301.139781E-437.49999632
ACGTTAG301.139781E-437.4999961
CGGTCTA601.546141E-1037.49999631
TATCGCG301.139781E-437.4999961
Produced by FastQC (version 0.11.5)