FastQCFastQC Report
Thu 26 May 2016
SRR1547061_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547061_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences928165
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43010.4633874365010532No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC10220.11010973264451902No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTACG551.8189894E-1245.0000041
CGTACGT207.0325856E-445.031
TACGTTA207.0325856E-445.033
CGTAAAC302.1650485E-644.99999610
AATGCGG1150.043.0434762
ACGGGTA950.042.631585
CGTTTTT29600.042.415541
CGGTCTA750.042.00000431
GACCGAT1450.041.8965539
CTATGCG556.002665E-1140.9090921
TTACGGG3300.039.5454523
GTTTACG403.4572622E-739.3751
CGTAAGG1600.039.3752
TCACGAG356.246988E-638.571431
TGTTACG356.246988E-638.571431
TTACCGG356.246988E-638.571432
AGGGCGA4800.038.4374966
GGGCGAT10750.038.3023267
TAGACGG1000.038.252
TCGTAAG659.094947E-1238.076921
Produced by FastQC (version 0.11.5)