##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547061_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	928165
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.654566806548406	31.0	31.0	33.0	30.0	34.0
2	32.05201984560935	33.0	31.0	34.0	30.0	34.0
3	32.1626316441581	33.0	31.0	34.0	30.0	34.0
4	35.829819051569494	37.0	35.0	37.0	35.0	37.0
5	35.77355534845636	37.0	35.0	37.0	35.0	37.0
6	35.82666120786713	37.0	35.0	37.0	35.0	37.0
7	36.11603648058266	37.0	35.0	37.0	35.0	37.0
8	36.06647417215689	37.0	35.0	37.0	35.0	37.0
9	37.86432692463086	39.0	38.0	39.0	35.0	39.0
10	37.38607898380137	39.0	37.0	39.0	35.0	39.0
11	37.1214353051451	39.0	37.0	39.0	33.0	39.0
12	36.42997527379291	38.0	35.0	39.0	33.0	39.0
13	36.1305565282035	39.0	35.0	39.0	32.0	39.0
14	37.17147489939828	39.0	35.0	41.0	32.0	41.0
15	37.379978775325505	39.0	35.0	41.0	33.0	41.0
16	37.48746397461658	39.0	35.0	41.0	33.0	41.0
17	37.457205346032225	39.0	35.0	41.0	33.0	41.0
18	37.35980456061153	39.0	35.0	41.0	33.0	41.0
19	37.32482155651204	39.0	35.0	41.0	33.0	41.0
20	37.1500304363987	39.0	35.0	41.0	32.0	41.0
21	37.08497627038296	39.0	35.0	41.0	32.0	41.0
22	37.01193106829066	39.0	35.0	41.0	32.0	41.0
23	36.978326051941195	38.0	35.0	41.0	32.0	41.0
24	36.894663125629606	38.0	35.0	41.0	32.0	41.0
25	36.79252072637947	38.0	35.0	41.0	32.0	41.0
26	36.64686235744722	38.0	35.0	41.0	31.0	41.0
27	36.55295340806861	38.0	35.0	41.0	31.0	41.0
28	36.59292474937107	38.0	35.0	41.0	31.0	41.0
29	36.61497794034466	38.0	35.0	41.0	31.0	41.0
30	36.561041409663154	38.0	35.0	41.0	31.0	41.0
31	36.40614761384021	38.0	35.0	41.0	31.0	41.0
32	36.32505642854449	38.0	35.0	41.0	31.0	41.0
33	36.204561688923846	38.0	35.0	41.0	30.0	41.0
34	36.059178055625885	38.0	35.0	41.0	30.0	41.0
35	35.96949464804211	38.0	35.0	41.0	30.0	41.0
36	35.874819671071414	38.0	35.0	41.0	30.0	41.0
37	35.8068651586733	38.0	35.0	41.0	30.0	41.0
38	35.73812845776344	38.0	35.0	41.0	29.0	41.0
39	35.69454676700802	38.0	35.0	40.0	29.0	41.0
40	35.55823264182554	38.0	35.0	40.0	29.0	41.0
41	35.51721191813955	37.0	35.0	40.0	29.0	41.0
42	35.43474705467239	37.0	35.0	40.0	28.0	41.0
43	35.31413811121945	37.0	34.0	40.0	28.0	41.0
44	35.18775756465715	37.0	34.0	40.0	28.0	41.0
45	35.09583964058115	37.0	34.0	40.0	27.0	41.0
46	35.04512451988601	36.0	34.0	40.0	27.0	41.0
47	35.01084936406781	36.0	34.0	40.0	27.0	41.0
48	34.93843443784241	36.0	34.0	40.0	27.0	41.0
49	34.875079323180685	36.0	34.0	40.0	27.0	41.0
50	34.76449445949804	36.0	34.0	40.0	27.0	41.0
51	34.39381683213653	35.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	2.0
13	4.0
14	13.0
15	29.0
16	59.0
17	152.0
18	360.0
19	654.0
20	1209.0
21	1814.0
22	2725.0
23	4186.0
24	5967.0
25	8831.0
26	11914.0
27	14395.0
28	15672.0
29	16929.0
30	19875.0
31	24098.0
32	30134.0
33	39846.0
34	75735.0
35	131521.0
36	60481.0
37	80060.0
38	124444.0
39	256874.0
40	180.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.9145141219503	23.621769836182143	29.264839764481533	14.198876277386024
2	30.638948893785052	25.195627932533547	30.708117629947264	13.45730554373414
3	29.60551195100009	25.10501904295034	31.388384608340115	13.90108439770946
4	26.450684953645094	27.438763581906233	31.18443380217957	14.9261176622691
5	24.144844935975822	31.990217256630014	29.59592313866608	14.269014668728081
6	22.422952815501553	40.546885521432074	26.153431771290663	10.876729891775708
7	87.52398549826809	4.021375509742342	6.686095683418358	1.7685433085712132
8	88.89324635167239	2.8301002515716496	6.300711619162541	1.9759417775934236
9	85.06170777825064	4.01318731044588	8.02669783928504	2.898407072018445
10	48.62098872506505	23.302753282013438	15.783616059644567	12.29264193327695
11	42.14961779424994	22.294204155511142	20.656456556754456	14.899721493484455
12	36.82050066529119	20.975904068780874	24.85236999886874	17.3512252670592
13	24.188156200675525	31.11170966369126	26.121648629284667	18.578485506348546
14	18.405886884336297	34.059676889346186	28.05891193914875	19.47552428716877
15	18.524723513599415	22.999035731793377	40.975580850387594	17.500659904219614
16	21.429271735090204	20.508853490489297	38.830595853108015	19.231278921312484
17	22.36100262345596	20.327743450787306	27.72405768370925	29.58719624204748
18	24.692915591516595	23.706022097364155	30.68010536919621	20.92095694192304
19	28.731098457709564	24.958708850258304	25.711699967139463	20.598492724892665
20	30.572689123162366	24.456535206563487	24.779645860380427	20.191129809893717
21	26.02586824540895	26.307391465957025	27.370241282530582	20.296499006103442
22	24.36290961197632	23.404890294290347	27.85679270388347	24.375407389849865
23	22.872657340020364	26.490871773876414	26.941869171968346	23.69460171413488
24	23.41458684608879	23.83875711753837	32.62728070978759	20.11937532658525
25	21.647228671626273	24.513529383245437	31.073677632748485	22.7655643123798
26	20.41490467750885	29.919464750340723	27.058335533014066	22.60729503913636
27	21.00822590810901	27.96517860509715	29.415028577892937	21.611566908900894
28	18.938766275392847	26.86052587632587	33.755851599661696	20.44485624861959
29	20.133920154282915	25.39203697618419	32.67113067180943	21.802912197723465
30	22.77730791400236	26.08695652173913	30.434782608695656	20.700952955562858
31	27.685379215979918	24.79903896397731	25.8619965200153	21.653585300027473
32	28.758141063280775	25.92265383848777	25.226764637752986	20.09244046047847
33	26.857616910786337	26.21344265297657	25.740574143605933	21.18836629263116
34	21.907311738753346	26.530950854643304	28.559146272483883	23.00259113411947
35	22.042524766609386	27.09270442216632	28.657835621899125	22.206935189325176
36	27.461388869435932	26.57275376684102	25.363809236504288	20.602048127218758
37	22.981581938556182	29.56629478594862	26.227879741209808	21.224243534285392
38	22.80693626671982	29.94489126394553	24.005537808471555	23.2426346608631
39	22.8922659225461	28.71816972197831	25.267597894770866	23.121966460704723
40	24.958277892400595	25.347001880053654	25.22633367989528	24.46838654765047
41	20.31826237791772	25.18539268341297	26.208163419219645	28.28818151944967
42	23.23552385621091	26.496474226026624	24.9153975855586	25.352604332203864
43	22.933314658492833	26.27528510555774	25.943016597264496	24.848383638684933
44	21.79149181449419	28.139716537469095	26.847166182736903	23.221625465299812
45	20.89671556242694	30.365721611997863	24.373037121632468	24.364525703942725
46	23.490327689581054	28.904020298115096	26.366109474069805	21.239542538234044
47	22.725054273755205	26.14610548770962	27.345999903034485	23.782840335500694
48	23.509505314249083	24.25215344254524	28.858338765198	23.380002478007682
49	22.8731960373425	23.49980876245064	29.134475012524714	24.492520187682146
50	21.005855639891614	28.53328880102137	26.788986871946257	23.67186868714076
51	20.612822073661473	29.776817699439217	25.167615671782496	24.442744555116818
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	409.0
1	803.0
2	1197.0
3	4619.5
4	8042.0
5	5723.0
6	3404.0
7	3386.0
8	3368.0
9	3534.5
10	3701.0
11	3665.0
12	3629.0
13	3534.0
14	3439.0
15	3373.0
16	3307.0
17	3182.5
18	3058.0
19	2835.5
20	2613.0
21	2793.0
22	2973.0
23	3121.5
24	3270.0
25	3752.0
26	4987.0
27	5740.0
28	6900.0
29	8060.0
30	9337.0
31	10614.0
32	12587.5
33	14561.0
34	16950.5
35	19340.0
36	22618.0
37	25896.0
38	29317.5
39	32739.0
40	42787.0
41	52835.0
42	61444.5
43	70054.0
44	71486.5
45	72919.0
46	72245.5
47	71572.0
48	71220.0
49	70868.0
50	69944.5
51	69021.0
52	66261.0
53	63501.0
54	58585.5
55	53670.0
56	50190.5
57	46711.0
58	45883.5
59	45056.0
60	43329.0
61	41602.0
62	38481.5
63	35361.0
64	29902.0
65	24443.0
66	20722.0
67	17001.0
68	14049.0
69	11097.0
70	9250.0
71	7403.0
72	6285.0
73	5167.0
74	4146.0
75	2446.0
76	1767.0
77	1282.0
78	797.0
79	599.5
80	402.0
81	244.0
82	86.0
83	74.5
84	63.0
85	47.5
86	32.0
87	20.0
88	8.0
89	6.0
90	4.0
91	2.5
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	1.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	928165.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.584580328757703
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.27691699144255	18.22543656343418
2	5.69374950464622	2.6856938512830633
3	1.9877351825420386	1.4063970025488173
4	1.1357727043860548	1.0714689032721314
5	0.8626477199070615	1.0172592222783883
6	0.6840594476536789	0.9679952995700288
7	0.6218040649431128	1.0265491542879241
8	0.5266246234484252	0.993617658785693
9	0.5006082993665651	1.0625972984688188
>10	9.920362271689905	59.068657252783666
>50	0.759380734483566	10.81410517098851
>100	0.02941910835480698	1.0831451103165581
>500	0.0	0.0
>1k	9.193471360877181E-4	0.5770775119822878
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4301	0.4633874365010532	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1022	0.11010973264451902	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.039217165051472526	0.0
2	0.0	0.0	0.0	0.13036475195681801	0.0
3	0.0	0.0	0.0	0.19048337310715227	0.0
4	0.0	0.0	0.0	0.27128797142749406	0.0
5	0.0	0.0	0.0	0.4726530304417857	0.0
6	0.0	0.0	0.0	0.7339212316775573	0.0
7	0.0	0.0	0.0	0.9084591640494956	0.0
8	0.0	0.0	0.0	1.366675106258047	0.0
9	0.0	0.0	0.0	1.5918505869107324	0.0
10	0.0	0.0	0.0	1.9087123517908993	0.0
11	0.0	0.0	0.0	2.1944374114516276	0.0
12	0.0	0.0	0.0	2.4452548846379685	0.0
13	0.0	0.0	0.0	2.556872969784467	0.0
14	0.0	0.0	0.0	2.608695652173913	0.0
15	0.0	0.0	0.0	2.6750631622610204	0.0
16	0.0	0.0	0.0	2.8157709028028424	0.0
17	0.0	0.0	0.0	2.967791287109512	0.0
18	0.0	0.0	0.0	3.171418874876773	0.0
19	0.0	0.0	0.0	3.274202323940248	0.0
20	0.0	0.0	0.0	3.3950860030274788	0.0
21	0.0	0.0	0.0	3.5424735903637825	0.0
22	0.0	0.0	0.0	3.6938475378838893	0.0
23	0.0	0.0	0.0	3.8664461598961393	0.0
24	0.0	0.0	0.0	4.004244934898429	0.0
25	0.0	0.0	0.0	4.132454897566705	0.0
26	0.0	0.0	0.0	4.247305166646017	0.0
27	0.0	0.0	0.0	4.36549535912257	0.0
28	0.0	0.0	0.0	4.487025474996364	0.0
29	0.0	0.0	0.0	4.616743790166619	0.0
30	0.0	0.0	0.0	4.792790075040537	0.0
31	0.0	0.0	0.0	4.935437125942047	0.0
32	0.0	0.0	0.0	5.082286015956215	0.0
33	0.0	0.0	0.0	5.222347319711473	0.0
34	0.0	0.0	0.0	5.373829006696008	0.0
35	0.0	0.0	0.0	5.551922341394041	0.0
36	0.0	0.0	0.0	5.709760656779775	0.0
37	0.0	0.0	0.0	5.874817516282127	0.0
38	0.0	0.0	0.0	6.065408628853706	0.0
39	0.0	0.0	0.0	6.317411236148745	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATTACG	55	1.8189894E-12	45.000004	1
CGTACGT	20	7.0325856E-4	45.0	31
TACGTTA	20	7.0325856E-4	45.0	33
CGTAAAC	30	2.1650485E-6	44.999996	10
AATGCGG	115	0.0	43.043476	2
ACGGGTA	95	0.0	42.63158	5
CGTTTTT	2960	0.0	42.41554	1
CGGTCTA	75	0.0	42.000004	31
GACCGAT	145	0.0	41.896553	9
CTATGCG	55	6.002665E-11	40.909092	1
TTACGGG	330	0.0	39.545452	3
GTTTACG	40	3.4572622E-7	39.375	1
CGTAAGG	160	0.0	39.375	2
TCACGAG	35	6.246988E-6	38.57143	1
TGTTACG	35	6.246988E-6	38.57143	1
TTACCGG	35	6.246988E-6	38.57143	2
AGGGCGA	480	0.0	38.437496	6
GGGCGAT	1075	0.0	38.302326	7
TAGACGG	100	0.0	38.25	2
TCGTAAG	65	9.094947E-12	38.07692	1
>>END_MODULE