FastQCFastQC Report
Thu 26 May 2016
SRR1547060_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547060_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1215519
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54180.44573552531881444No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC17300.14232603521623272No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG207.0334476E-445.01
ATTCGTT207.0334476E-445.010
ATACGGA253.890939E-545.012
TCGACTG207.0334476E-445.01
CGATACG406.8157533E-945.010
AATCGGA207.0334476E-445.017
AAGTCGC207.0334476E-445.032
ACGCCGT302.165605E-644.99999642
CGTTTTT32800.042.5990871
TACGTAG700.041.7857131
TAGGGTC2450.041.326535
CTTAACG603.6379788E-1241.2499961
CGAACGG1150.041.0869562
CGGGTAT451.9284926E-840.06
CGACGGT1250.039.628
ACTAACG403.4584446E-739.37530
TAGCACG750.039.01
GCGATAC700.038.5714269
GTAGGGA9300.038.4677434
CTCACGA1300.038.0769224
Produced by FastQC (version 0.11.5)